SPE

Combination phase for SPE of hydrocarbons from water acc. to DIN H53 / ISO DIS 9377-4

Special combination phase of sodium sulphate and Florisil^®.

Recommended application: hydrocarbons from drinking, surface and waste waters.

Octadecyl modified silica phase for SPE, endcapped

Base material silica, pore size 60 Å, particle size 45 µm for C₁₈ ec, 100 µm for C₁₈ ec f (for fast flow), specific surface 500 m²/g, pH stability 2 to 8. Octadecyl phases, endcapped, carbon content 14 % very non-polar, hydrophobic interactions with a wide variety of organic compounds. Advantageous for clean-up of samples with large structural variations (polarity differences).

Recommended applications: non-polar compounds, aflatoxins, amphetamines, antibiotics, antiepileptics, barbiturates, caffeine, drugs, preservatives, fatty acids, nicotine, PAHs, pesticides, PCBs, heavy metals, vitamins. Very well suited for desalting of samples.

For simultaneous preparation of up to 12 or 24 samples,
replacement parts and accessories for special applications.

Vacuum manifold complete consists of: glass cabinet with lid and lid gasket, removable needles on lower side of lid, vacuum gauge, control valve, valves and caps, variable rack.

Aminopropyl modified silica phase for SPE

Base material silica, pore size 60Å, particle size 45µm, specific surface 500m²/g, pH stability 2 to 8, aminopropyl phase, carbon content 3.5%, polar, weak anion exchanger.

Recommended applications: trace elements, lipids.

Combination phase for SPE analysis of PAH from water containing humic acids

Special combination phase: aminopropyl phase for removal of interfering humic acids, octadecyl phase for enrichment of PAH.

Recommended application: PAH from water containing humic acids.

QuEChERS method and pre-mixes
Within a few years after its devleopment by Anastassiades et al. the QuEChERS method has gained a leading position for determination of pesticide residues in food by GC-MS or LC-MS allowing rapid and cheap clean-up of strong matrix-contaminated samples.
Standard clean-up of food samples
10 g sample are homogenised with 10 ml acetonitrile. After adding the internal standard the sample is shaken with 4 g MgSO₄ and 1 g NaCI and afterwards centrifuged. 1 ml of the supernantant is spiked with 25 mg CHROMABOND^® Diamino and 150 mg MgSO₄ and shaken again. After centrifugation the supernatant is injected into the GC/MS.
Extraction mixtures for sample preparation for determination of pesticides in food samples.

MACHEREY-NAGEL offers a number of individually weighed and pre-mixed buffers and extraction mixtures, specially composed for different sample matrices.
Mix I and Mix II are extraction mixtures, Mix III to Mix VI are clean-up mixtures.

The food samples will be extracted with either Mix I or Mix II.
Afterwards they will be purified with one of the following mixtures:

• Mix III (samples with low fat content; e.g. apples, strawberries),
• Mix IV (moderate content of chlorophyll and carotinoids; e. g. carrots, lettuce),
• Mix V (high content of chlorophyll and carotinoids; e. g. bell peppers, spinach),
• Mix VI (high fat content; e. g. avocados).

Unmodified silica phase for SPE

Unmodified, weakly acidic silica, pore size 60Å, particle size 45µm, specific surface 500m²/g, pH stability 2 to 8, very polar, adsorbs humidity from air, for this reason it should be kept well closed and if necessary dried before use due to its high affinity for polar compounds it should not be conditioned with polar (e.g. methanol) or water-containing solvents.

Recommended applications: aflatoxins, chloramphenicol, pesticides, steroids, vitamins.

Extraction and clean-up mixtures for sample preparation in the pesticide analysis of vegetable foodstuffs according to EN 15662 using GC/MS or LC/MS after acetonitrile extraction and purification using the dispersive SPE-QuEChERS method.

The samples are first extracted with Mix A. Purification is done with one of the following mixtures:

• Mix B (Clean-up mixture for fruits and vegetables with low fat content, e.g. apples, strawberries)
• Mix C (Clean-up mixture for fruits and vegetables with high fat content, containing oil/wax, e.g. avocados)
• Mix D (Clean-up mixture for moderately chlorophyll/carotenoid-containing fruits and vegetables, e.g. carrots, salad)
• Mix E (Clean-up mixture for fruits and vegetables with high chlorophyll/carotenoid content, e.g. sweet peppers, spinach)

• Mix A contains a bag with the required amount of mixture and a 50 ml empty tube
• Mix B, C, D and E are pre-weighed into a 15 ml tube

Octadecyl modified silica phase for SPE, not endcapped

Base material silica, pore size 60 Å, particle size 45 µm for C₁₈, specific surface 500 m²/g, pH stability 2 to 8 octadecyl phases, not endcapped, carbon content 14 % possesses more free silanols (SiOH), which allow secondary interactions with polar groups of the analytes.

Recommended applications: non-polar compounds, pesticides.