HPLC accessories

Support materials for TLC ready-to-use layers

Glass plates: glass, ~ 1.3 mm thick, high requirements for weight, packaging and storage, ideal torsional strength, high temperature stability, susceptible to breakage, can not be cut with scissors, high resistance against solvents, mineral acids and conc. ammonia, suitability for aqueous detection reagents depends on the phase

POLYGRAM^®: polyester, ~ 0.2 mm thick, low requirements for weight, packaging and storage, low torsional strength, max. 185 °C temperature stability, not susceptible to breakage, can be cut with scissors, high resistance against solvents, mineral acids and conc. ammonia, very suitable for aqueous detection reagents

ALUGRAM^®: aluminium, ~ 0.15 mm thick, low requirements for weight, packaging and storage, relatively high torsional strength, high temperature stability, not susceptible to breakage, can be cut with scissors, high resistance against solvents, low resistance against mineral acids and conc. ammonia, limited suitability for aqueous detection reagents
Glass plates, POLYGRAM^®, ALUGRAM^®

• Silica 60
• Specific surface (BET) ~500 m²/g
• Mean pore size 60 Å
• Specific pore volume 0.75 ml/g
• Particle size 5 to 17 µm
• Standard grade
• Thickness of layer for analytical plates 0.25 mm, for preparative plates 0.5 and 1 mm
• For 2 mm preparative layers a slightly coarser material is used, indicators: manganese activated zinc silicate with green fluorescence for short-wave UV (254 nm)
• Binders: highly polymeric products, which are stable in almost all organic solvents and resistant towards aggressive visualisation reagents
• Binder system for Polygram^® sheets is also completely stable in purely aqueous eluents
• Available as glass plates, Polygram polyester sheets and Alugram aluminium sheets
• Available with or without fluorescent indicator (UV 254)

Whatman chromatography papers are the most widely used papers for chromatography worldwide. This acceptance and usage reflects the purity, high quality and consistency of Whatman papers. These qualities are relied upon by chromatographers and essential to successful reproducible chromatography. Whatman chromatography paper media are made from specially selected cotton cellulose. They are rigorously quality controlled for characteristics important to the chromatographer and to ensure uniformity within the grade.
1 Chr world standard chromatography paper. A smooth surface, 0.18 mm thick with a linear flow rate (water) of 130 mm/30 min. Good resolution for general analytical separations.
3MM Chr widely used as a blotting paper, 3MM Chr is used in both electrophoresis and for general chemistry. A medium thickness paper (0.34 mm) used extensively for general chromatography and electrophoresis. Flow rate is 130 mm/30 min.
3 Chr medium thickness paper (0.34 mm) with a flow rate of 130 mm/30 min. For general applications with medium/heavy solute loadings. Frequently used for separation of inorganic compounds and for electrophoresis.
17 Chr thick (0.92 mm) and highly absorbent paper with a very high flow rate of 190 mm/30 min. Suitable for the heaviest loadings and ideal for preparative paper chromatography and electrophoresis.

Ion exchange paper
SG81: A unique paper (0.27 mm thick) combining cellulose and large pore silica gel. Suitable for separations in which both partition and adsorption are important, including the separation of phospholipids, steroids, phenols and dyes. Flow rate is 110 mm/30 min.

Support materials for TLC ready-to-use layers

Glass plates: glass, ~ 1.3 mm thick, high requirements for weight, packaging and storage, ideal torsional strength, high temperature stability, susceptible to breakage, can not be cut with scissors, high resistance against solvents, mineral acids and conc. ammonia, suitability for aqueous detection reagents depends on the phase

POLYGRAM^®: polyester, ~ 0.2 mm thick, low requirements for weight, packaging and storage, low torsional strength, max. 185 °C temperature stability, not susceptible to breakage, can be cut with scissors, high resistance against solvents, mineral acids and conc. ammonia, very suitable for aqueous detection reagents

ALUGRAM^®: aluminium, ~ 0.15 mm thick, low requirements for weight, packaging and storage, relatively high torsional strength, high temperature stability, not susceptible to breakage, can be cut with scissors, high resistance against solvents, low resistance against mineral acids and conc. ammonia, limited suitability for aqueous detection reagents
Silica 60, specific surface (BET) ~ 500m²/g, mean pore size 60Å, specific pore volume 0.75ml/g, particle size 5 to 17µm. Outstanding hardness and abrasion resistance due to an optimized binder system. Increased separation efficiency due to an optimized particle size distirbution. High suitability for trace analyses resulting from a UV indicator with brilliance and a low-noise background of the layer.

Available as glass plates with or without fluorescent indicator (UV254).

Support materials for TLC ready-to-use layers

Glass plates: glass, ~ 1.3 mm thick, high requirements for weight, packaging and storage, ideal torsional strength, high temperature stability, susceptible to breakage, can not be cut with scissors, high resistance against solvents, mineral acids and conc. ammonia, suitability for aqueous detection reagents depends on the phase

POLYGRAM^®: polyester, ~ 0.2 mm thick, low requirements for weight, packaging and storage, low torsional strength, max. 185 °C temperature stability, not susceptible to breakage, can be cut with scissors, high resistance against solvents, mineral acids and conc. ammonia, very suitable for aqueous detection reagents

ALUGRAM^®: aluminium, ~ 0.15 mm thick, low requirements for weight, packaging and storage, relatively high torsional strength, high temperature stability, not susceptible to breakage, can be cut with scissors, high resistance against solvents, low resistance against mineral acids and conc. ammonia, limited suitability for aqueous detection reagents
ALUGRAM^®
Nano silica 60, specific surface (BET) ~ 500m²/g, mean pore size 60Å, specific pore volume 0.75ml/g, particle size 2 to 10µm. Indicator: manganese activated zinc silicate with green fluorescence for short-wave UV (254nm).

Binder: highly polymeric product, which is stable in almost all organic solvents and resistant towards aggressive visualisation reagents. Narrow fractionation of the silica particles allows sharper separations, shorter developing times, shorter migration distances, smaller samples and an increased detection sensitivity compared to SIL G plates.

Available as glass plates with or without fluorescent indicator (UV254).

The primary field of application is the partition chromatography of polar substances such as amino acids, carboxylic acids or carbohydrates. The TLC-plates are available as glass plates, POLYGRAM^® polyester sheets and ALUGRAM^® aluminium sheets.

• Fiber length (95 %) 2-20 µm
• Average degree of polymerization 400-500
• Specific surface according to Blaine 15000 cm²/g
• ≤ 20 ppm Fe, 6 ppm Cu, 7 ppm P
• Dichloromethane extract ≤ 0.25 %
• Residue on ignition at 850 °C ≤ 1500 ppm
• Available with or without fluorescent indicator (UV 254)

Standard, rigid TLC plates in a choice of media, backing materials, and with dimensions as outlined below.

Recommended for chromatographic analysis and preparations.

• Made from pure linters with an α-cellulose content of > 98%
• High-performance resolution and wet strength
• The fibres are oriented in predominantly one direction
• Thicker papers allow higher sample volumes
• Lower capillary rises offer higher resolutions

Chromatography Paper 1 CHR
The standard chromatography paper. Good resolution for general analytical separations. Pure cellulose. Thickness 0.18mm. Capillary rise (water) 130mm / 30 min.

Chromatography Paper 3MM CHR
Used in electophoresis, in general chemistry and as blotting paper. Pure cellulose. Thickness 0.34mm.
Capillary rise (water) 130mm / 30 min.

Divided into 12 bands, each of 15mm wide, for parallel separation of 12 samples.

Support materials for TLC ready-to-use layers

Glass plates: glass, ~ 1.3 mm thick, high requirements for weight, packaging and storage, ideal torsional strength, high temperature stability, susceptible to breakage, can not be cut with scissors, high resistance against solvents, mineral acids and conc. ammonia, suitability for aqueous detection reagents depends on the phase

POLYGRAM^®: polyester, ~ 0.2 mm thick, low requirements for weight, packaging and storage, low torsional strength, max. 185 °C temperature stability, not susceptible to breakage, can be cut with scissors, high resistance against solvents, mineral acids and conc. ammonia, very suitable for aqueous detection reagents

ALUGRAM^®: aluminium, ~ 0.15 mm thick, low requirements for weight, packaging and storage, relatively high torsional strength, high temperature stability, not susceptible to breakage, can be cut with scissors, high resistance against solvents, low resistance against mineral acids and conc. ammonia, limited suitability for aqueous detection reagents
Glass plates

nano silica 60, specific surface (BET) ~ 500m²/g, mean pore size 60Å, specific pore volume 0.75ml/g,
particle size 2 to 10 µm
outstanding hardness and abrasion resistance due to an optimized binder system increased separation efficiency due to an optimized particle size distribution high suitability for trace analyses resulting from a UV indicator with increased brilliance and a low-noise background of the layer narrow fractionation of the silica particles allows theoretical plate heights, which are one order of magnitude smaller than on standard silica layers with the advantage of sharper separations, shorter developing times, shorter migration distances, lower amount of samples, and increased detection sensitivity with equal selectivity.

Available as glass plates with or without fluorescent indicator (UV 254).

Silica gel 60 F₂₅₄

ALUGRAM^®

Base material: Nano-K silica 60, specific surface (BET) ~500 m²/g, mean pore size 60 Å, specific pore volume 0.75 ml/g, particle size 2 to 10 µm; for preparative plates (1 mm thickness of layer) standard silica 60, particle size 5 to 17 µm, pH stability 2 to 10 indicator: acid-resistant product with a pale blue fluorescence for short-wave UV (254 nm); UV-absorbing substances apppear as dark-blue to black spots on a light-blue background; partial octadecyl modification, wettable with water, carbon content 14 %. 18-100 normal phase or reversed phase separation modes with eluents from anhydrous solvents to mixtures with high concentrations of water (see figure); the relative polarity of the eluent determines the polarity of the layer.
Recommended application: aminophenols, barbiturates, preservatives, nucleobases, polycyclic aromatic hydrocarbons, steroids, tetracyclines, plasticizers (phthalates).

Available as glass plates with or without fluorescent indicator (UV 254).

Glass plates available on request.

Support materials for TLC ready-to-use layers

Glass plates: glass, ~ 1.3 mm thick, high requirements for weight, packaging and storage, ideal torsional strength, high temperature stability, susceptible to breakage, can not be cut with scissors, high resistance against solvents, mineral acids and conc. ammonia, suitability for aqueous detection reagents depends on the phase

POLYGRAM^®: polyester, ~ 0.2 mm thick, low requirements for weight, packaging and storage, low torsional strength, max. 185 °C temperature stability, not susceptible to breakage, can be cut with scissors, high resistance against solvents, mineral acids and conc. ammonia, very suitable for aqueous detection reagents

ALUGRAM^®: aluminium, ~ 0.15 mm thick, low requirements for weight, packaging and storage, relatively high torsional strength, high temperature stability, not susceptible to breakage, can be cut with scissors, high resistance against solvents, low resistance against mineral acids and conc. ammonia, limited suitability for aqueous detection reagents
Glass plates

silica 60, specific surface (BET) ~ 500m²/g, mean pore size 60Ä, specific pore volume 0.75ml/g, particle size 5.17µm
kieselguhr zone for rapid sample application: because kieselguhr is completely inert towards a large number of compounds, the samples always from a narrow band at the interface of the two adsorbents, irrespective of shape, size or position of the spots in the concentrating zone. Separation then takes place in
the silica layer.

available as glass plates with or without fluorescent indicator (UV254)

Features separations with simple developing sovents; samples are coloured thus eliminating the need for visualisation. All equipment needed is contained in the kit.

Inorganic fluorescent indicators, green fluorescing.

• V-shape provides optimal recovery of reagents
• Shared wall technology increases well volume and wider cells improve mixing
• Ideal as collection plate for Nunc Filter Plates
• Low binding for homogeneous assays and storage

Cell culture multiwell plates are available in the hydrophilic surface (TC surface treatment) for improved cell adhesion and hydrophobic surface for suspension cultures and hybridoma cells. All panels can be stacked together, both with and without cover. The special design of the panels is also in stacked plates a faster heat exchange over the entire bottom surface of each plate allows . All plates have an imprinted lot number and are both pyrogens and DNase / RNase - free .

Properties:

• High clarity and low autofluorescence
• Lid enables optimal gas exchange with the lowest possible evaporation
• Single position lids to prevent cross-contamination
• Alphanumeric well coding
• Compatible with common instruments and automated systems
• Easy-to-open packaging

With 8 x 12 wells. Coded: A to H and 1 to 12. Volume per well 0.3 ml. Can be sealed by accessory cover plates (individually heat-sealed) or adhesive film from a roll.

Three PCR instruments in one - the Biometra TRIO offers three separate sample blocks with which you can perform three different PCR reactions simultaneously. The simultaneous use of all three blocks also enables a very high throughput.
The Biometra TRIO is ideal for optimizing primer annealing temperatures, which are crucial for specific and efficient PCR reactions. The professional user management is ideal for laboratories with many users.

• Highest flexibility due to three powerful block modules
• Short protocol run times thanks to fast heating and cooling rates
• Best reproducibility due to highest temperature uniformity
• Easy determination of the optimum annealing temperature thanks to Temperature Optimization Step technology
• Protocol Wizard and annealing temperature calculator
• Simple opening mechanism and direct access to the sample block
• Whisper-quiet operation thanks to optimized air flow
• Advanced user management
• PCR Control App

Durapore® PVDF membranes are characterized by high throughput and high flow rate. To maximize recovery and minimize interaction with your sample, the membrane has very low protein binding.

Features & Benefits:

• Chemical compatibility
• Automation compatible
• Suitable for: Receptor/Ligand Binding Assay, Protein Kinase/Phosphatase Precipitation Assays, Bead-Based Assay