GC reagents

For improved volatility, better thermal stability or low limit of detection in gas chromatography prerequisite: quantitative, rapid and reproducible formation of only on derivative halogen atoms introduced by derivatisation (e.g. trifluoroacetates) allow specific detection (ECD) with the advantage of high sensitivity elution order and fragmentation patterns in MS can be influenced by a specific derivatisation.
Reagents for silylation - alkylation (methylation) - acylation are available.
TMSH (0.2 M in methanol) M.G. 94.06

Please Note: These products will only operate with the correct type of air compressor and gas regulator as specified below.

For improved volatility, better thermal stability or low limit of detection in gas chromatography prerequisite: quantitative, rapid and reproducible formation of only on derivative halogen atoms introduced by derivatisation (e.g. trifluoroacetates) allow specific detection (ECD) with the advantage of high sensitivity elution order and fragmentation patterns in MS can be influenced by a specific derivatisation.
Reagents for silylation - alkylation (methylation) - acylation are available.
Which is the proper reagent for acylation?
Test kit for determination of the optimum acylation.

For improved volatility, better thermal stability or low limit of detection in gas chromatography prerequisite: quantitative, rapid and reproducible formation of only on derivative halogen atoms introduced by derivatisation (e.g. trifluoroacetates) allow specific detection (ECD) with the advantage of high sensitivity elution order and fragmentation patterns in MS can be influenced by a specific derivatisation.
Reagents for silylation - alkylation (methylation) - acylation are available.
Acylation with fluorinated acid anhydrides can be used for alcohols, phenols, carboxylic acids, amines, amino acids and steroids forming volatile, stable derivatives suited for FID as well as for ECD detection.

by-products of acylation with anhydrides: corresponding acids
excess reagent and the acid formed have to be removed

Heptafluorobutyric acid anhydride (HFBA)
m.w. 410.06, Bp 106 - 107°C (760mm Hg),
C₃F₇ - CO - O - CO - C₃F₇
density d20°/4° = 1.665

For improved volatility, better thermal stability or low limit of detection in gas chromatography prerequisite: quantitative, rapid and reproducible formation of only on derivative halogen atoms introduced by derivatisation (e.g. trifluoroacetates) allow specific detection (ECD) with the advantage of high sensitivity elution order and fragmentation patterns in MS can be influenced by a specific derivatisation.
Reagents for silylation - alkylation (methylation) - acylation are available.
Acylation with fluorinated acid amides is recommended for alcohols, primary and secondary amines as well as for thiols under mild, neutral conditions. MBTFA also forms very volatile derivatives with carbohydrates.

by-products: corresponding neutral acylamides, which can be easily removed due to their high volatility; because of neutral conditions and favourable chromatographic properties often removal of the bisacylamide is not necessary. Thus sample preparation is much more convenient.

MBTFA / MBHFBA
N-methyl-bis(trifluoroacetamide) MBTFA
m.w. 223.08, Bp 123 - 124°C (760mm Hg), density d20°/4° = 1.55,
CF₃ - CO - N(CH₃) - CO - CF₃

For improved volatility, better thermal stability or low limit of detection in gas chromatography prerequisite: quantitative, rapid and reproducible formation of only on derivative halogen atoms introduced by derivatisation (e.g. trifluoroacetates) allow specific detection (ECD) with the advantage of high sensitivity elution order and fragmentation patterns in MS can be influenced by a specific derivatisation.
Reagents for silylation - alkylation (methylation) - acylation are available.
Which is the proper reagent for methylation?
Test kit for determination of the optimum alkylation.

For improved volatility, better thermal stability or low limit of detection in gas chromatography prerequisite: quantitative, rapid and reproducible formation of only on derivative halogen atoms introduced by derivatisation (e.g. trifluoroacetates) allow specific detection (ECD) with the advantage of high sensitivity elution order and fragmentation patterns in MS can be influenced by a specific derivatisation.
Reagents for silylation - alkylation (methylation) - acylation are available.
Which type of derivatisation is best suited for your sample (alkylation, silylation or acylation)? Test kit for determination of the optimum derivatisation.

For improved volatility, better thermal stability or low limit of detection in gas chromatography prerequisite: quantitative, rapid and reproducible formation of only on derivative halogen atoms introduced by derivatisation (e.g. trifluoroacetates) allow specific detection (ECD) with the advantage of high sensitivity elution order and fragmentation patterns in MS can be influenced by a specific derivatisation.
Reagents for silylation - alkylation (methylation) - acylation are available.
Which is the proper reagent for silylation?
Test kit for determination of the optimum silylation.

For improved volatility, better thermal stability or low limit of detection in gas chromatography prerequisite: quantitative, rapid and reproducible formation of only on derivative halogen atoms introduced by derivatisation (e.g. trifluoroacetates) allow specific detection (ECD) with the advantage of high sensitivity elution order and fragmentation patterns in MS can be influenced by a specific derivatisation.
Reagents for silylation - alkylation (methylation) - acylation are available.
N,O-bis-trimethylsilyl-trifluoroacetamide
m.w. 257.4, Bp 40°C (12mm Hg), density d20°/4° = 0.961
BSTFA: R = CF₃ powerful trimethylsilyl donor with approximately the same donor strength as the non-fluorinated analogue BSA
advantage of BSTFA over BSA: greater volatility of its reaction products (particularly useful for GC of some lower boiling TMS amino acids.

BSTFA is nonpolar (less polar than MSTFA), and can be mixed with acetonitrile for improved solubility. For silylating fatty acid amides, hindered hydroxyls and other compounds, which are difficult to silylate (like secondary alcohols and amines), we recommend BSTFA + 1% trimethylchlorosilane (TMCS), available under the designation SILYL-991.

For improved volatility, better thermal stability or low limit of detection in gas chromatography prerequisite: quantitative, rapid and reproducible formation of only on derivative halogen atoms introduced by derivatisation (e.g. trifluoroacetates) allow specific detection (ECD) with the advantage of high sensitivity elution order and fragmentation patterns in MS can be influenced by a specific derivatisation.
Reagents for silylation - alkylation (methylation) - acylation are available.
N-methyl-N-trimethylsilyl-trifluoroacetamide
m.w. 199.1, Bp 70 °C (75 mm Hg), density d20°/4° = 1.11
MSTFA: R' = CF₃, R" = CH₃
The most volatile trimethylsilyl amide available.
Very strong TMS donor which does not cause any noticeable fouling of the FID burning chamber even after long-time measuring series.
The already good solution characteristics can be improved by addition of submolar quantities of protic solvents (e.g. TFA for extremely polar compounds such as hydrochlorides) or pyridine (e.g. for carbohydrates).

Powder reagents in individually sealed packages.