SPE

96-well polypropylene plates for simultaneous filtration of 96 samples
Advantages of this high-throughput system are:

• economical by saving time and solvent
• use of multi-channel pipettors facilitates liquid transfer steps
• readily adaptable to all common automated/robotic handling systems
• minimised dead volume (≤ 40µl)
• membrane materials correspond to the respective CHROMAFIL^® syringe filters

Empty CHROMABOND^® column (PP) for SPE, one PE-filter element is already inserted in the column.

Kieselguhr phase for liquid-liquid extraction

Base material coarse-grained kieselguhr, large pore size, high pore volume, constantly high batch-to-batch quality, pH working range 1 to 13.

Application: liquid-liquid extraction of highly viscous aqueous solutions such as physiological fluids (blood, plasma, and serum) in clinical chemistry, dyes in textiles, environmental and food analysis.

Glass columns availlable on request.

Polar modified polystyrene-divinylbenzene copolymer with a weak anion exchanger

Specific surface 650 to 700 m²/g, particle size 80µm, pore size 50 Å, pH stability 1 to 14. Due to bifunctional modification much more hydrophilic than conventional polystyrene-divinylbenzene polymers and thus easily wettable with water.

Recommended applications: polar herbicides / pesticides from water (acidic, neutral, basic), polar phenols from water, polyaromatic compounds, polychlorinated biphenyls, drug analysis from urine, blood, serum, plasma pharmaceuticals / active ingredients from tablets, creams.

Due to the bifunctional modification CHROMABOND^® Easy is considerably more hydrophilic than conventional polystyrene-divinylbenzene polymers and thus easily wettable with water. The Easy effect: Aqueous samples can be loaded directly without preconditioning! This means that little or even no conditioning is needed, in contrast to standard SPE materials, where recovery rates normally decrease, in the worst case down to zero! Depending on the separation task conditioning may be required and is recommended for method development. A positive side effect of the excellent wettability: there is no decrease of recovery rates, if the cartridge runs dry, therefore automation is easier or, in some cases compared to silica materials, only feasible with CHROMABOND^® Easy, because a permanent vacuum can be used without supervision.

Further advantages of using a polymeric material:

• high surface, this means very high binding capacity (2 - 5 times higher than silica-based adsorbents)
• less adsorbent is needed in the cartridge (without losing sensitivity or recovery)
• faster analysis, because the height of the adsorbent bed can be reduced
• acidic or basic solvents (e. g. TFA) do not destroy the phase, or lead to unintended side products

Because of the polar modification the material is suitable for a broad range of compounds (acidic, neutral, basic, polar and nonpolar substances). Highly reproducible recovery rates can be obtained, even if the cartridge runs dry (especially advantageous when using 96-well plates, where stopcocks are not available!)

Magnesium silicate for SPE

Matrix magnesium silicate (MgO to SiOH 15:85), high purity, particle size 150 to 250µm.

Recommended application: organic tin compounds, aliphatic carboxylic acids, PCB, PAH.

Spherical, hydrophobic polystyrene-divinylbenzene resin for SPE
Hydrophobic polystyrene-divinylbenzene copolymer pH stability 1 to14. High-purity material with highest reproducibility and lowest blank values due to a novel manufacturing process, spherical particles 85mm; pore size 55 to 60Å. Very high surface 1000m²/g; capacity 390mg/g (caffeine in water). Excellent recovery rates especially for the enrichment of pharmaceuticals/active ingredients due to the spherical structure of the particles, very homogeneous surface, and optimised pore structure.

Recommended applications: pharmaceuticals/active ingredients from tablets, creams and water/waste water, drugs and pharmaceuticals from urine, blood, serum and plasma trace analysis of pesticides.

QuEChERS method and pre-mixes
Within a few years after its devleopment by Anastassiades et al. the QuEChERS method has gained a leading position for determination of pesticide residues in food by GC-MS or LC-MS allowing rapid and cheap clean-up of strong matrix-contaminated samples.
Standard clean-up of food samples
10 g sample are homogenised with 10 ml acetonitrile. After adding the internal standard the sample is shaken with 4 g MgSO₄ and 1 g NaCI and afterwards centrifuged. 1 ml of the supernantant is spiked with 25 mg CHROMABOND^® Diamino and 150 mg MgSO₄ and shaken again. After centrifugation the supernatant is injected into the GC/MS.
Empty PP centrifuge tube with screw cap, 50 ml, without QuEchERS mixtures.

Special silica phase for SPE of THC and derivatives acidic analytes from biological fluids (urine, blood ...)

base material silica, pore size 60Å, particle size 45µm, specific surface 500m²/g, pH stability 2 to 8, special bifunctional modification - C₈/ SB (strong anion exchanger - quaternary amine).

Recommended applications: extraction of THC and derivatives from urine, blood, serum, plasma, acidic analytes from biological fluids.

aluminium oxide, acidic, neutral, basic

aluminium oxide, high purity, pore volume 0.90ml/g, particle size 60 to 150µm, specific surface 150m²/g, acidic, pH 4 ± 0.5, recommended application: together with phase SA for PCB and pesticides.

Alox A: acidic, pH 4 ± 0.5
Alox N: neutral, pH 7 ± 0.5
Alox B: basic, pH 9.5 ± 0.5

SPE-columns.