Cell culture cultivation

The cell culture flasks from LABSOLUTE® are made exclusively of PS, corresponding to the highest international standards (USP Class VI). A short, wide and angled neck allows an easy handling while the risk of contamination is minimized. The treatment of surfaces allows adherent cells a uniform and even cell adhesion.

• Short and wide sloping neck for easy access
• Easily stackable
• Both flasks sides have engraved graduation with a labelling area
• Screw cap with a hydrophobic 0.22 μm filter for ventilation
• Packed upright in resealable bags
• Surface is treated with vacuum gas plasma
• Sterilized by radiation (SAL 10^-6)

Accessori Cell scrapers and cell lifters, sterile Alternativa Cell culture dishes, treated, sterile, PS Alternativa Cell culture multiwell plates, treated, sterile, PS

The LABSOLUTE® filtration units are ideal for filtration of buffer solutions, cell culture media, microbiological media and other biological fluids. The PES membrane guarantees good flow through times and low protein binding.

• High traceability thanks to expiry date and Lot. no. on each filter
• 0.2 μm: Ideal for sterilization applications
• 0.45 μm: Ideal for clarifying buffer solutions and nutrient media preparation
• Filtrate bottle with lid for convenient storage of the filtered solution
• Individually packaged
• Different volumes available
• Pyrogen free and not cytotoxic
• Quick connection for hoses with an inside diameter of 8 to 12 mm
• Gamma sterilized (SAL 10^-6)

The complete filtration unit consists of a filter funnel with vacuum connection and lid, the filtrate bottle and the associated screw cap.

Accessori Filter funnel, sterile, PES Accessori Filtrate bottle, sterile, PS

The LABSOLUTE® multiwell tissue culture plates are suitable for handling multiple samples in a single experiment. From 4-well up to 96-well plates, we offer you the appropriate multiwell plate for every experiment. Numbered wells and a rounded corner in the cover reduce misplacement and facilitate identification. The treatment of surfaces allows adherent cells a uniform and even cell adhesion.

• Evaporation is reduced by condensation rings
• Easily stackable
• Individually wrapped in paper/plastic blister pack
• Surface is treated with vacuum gas plasma
• Sterilized by radiation (SAL 10^-6)

Accessori Cell scrapers and cell lifters, sterile Alternativa Cell culture dishes, treated, sterile, PS Alternativa Cell culture flasks, treated, sterile, PS

The LABSOLUTE® filtration units are ideal for filtration of buffer solutions, cell culture media, microbiological media and other biological fluids. The PES membrane guarantees good flow through times and low protein binding.

• High traceability thanks to expiry date and Lot. no. on each filter
• 0.2 μm: Ideal for sterilization applications
• 0.45 μm: Ideal for clarifying buffer solutions and nutrient media preparation
• Filtrate bottle with lid for convenient storage of the filtered solution
• Individually packaged
• Different volumes available
• Pyrogen free and not cytotoxic
• Quick connection for hoses with an inside diameter of 8 to 12 mm
• Gamma sterilized (SAL 10^-6)

LABSOLUTE® filtrate bottle with ergonomic design for storing sterile solutions made of PS with a PE cap.

Accessori Filtration units, sterile, PES Accessori Filter funnel, sterile, PES

The LABSOLUTE® cell strainers are made of a strong nylon mesh and are characterized by an even distribution of the mesh pores. With the sterile cell sieves, cell suspensions can be quickly and easily filtered to collect primary cells or prepare samples for flow cytometry.

• Available in three mesh sizes: 40, 70 and 100 μm
• Color coded for easy distinction
• Extended edge allows easy handling even with tweezers
• Designed to fit standard 50 ml centrifuge tubes
• DNase, RNase and pyrogen-free
• Individually packed, gamma-irradiated (SAL 10^-6)

Accessori Centrifuge tubes, PP

The LABSOLUTE® filtration units are ideal for filtration of buffer solutions, cell culture media, microbiological media and other biological fluids. The PES membrane guarantees good flow through times and low protein binding.

• High traceability thanks to expiry date and Lot. no. on each filter
• 0.2 μm: Ideal for sterilization applications
• 0.45 μm: Ideal for clarifying buffer solutions and nutrient media preparation
• Filtrate bottle with lid for convenient storage of the filtered solution
• Individually packaged
• Different volumes available
• Pyrogen free and not cytotoxic
• Quick connection for hoses with an inside diameter of 8 to 12 mm
• Gamma sterilized (SAL 10^-6)

The filter funnels from LABSOLUTE® can be easily screwed onto all standard bottles with a 45 mm thread. The membrane type and pore size are imprinted for easy identification.

Accessori Filtration units, sterile, PES Accessori Filtrate bottle, sterile, PS

The LABSOLUTE® cell scrapers and lifters enable easy and efficient cell harvest. Optimize your cell harvest by using the cell scraper for cell culture flasks and cell lifter for dishes. The handle is made of ABS and allows the controlled use of the thin, flexible blade made of TPE.

• Scraper in two different handle lengths (25 cm with 2 cm blade and 39 cm with 3 cm blade)
• Two blade positions: scraper and lifter
• Individually wrapped in paper/plastic blister packs

Accessori Cell culture dishes, treated, sterile, PS Accessori Cell culture flasks, treated, sterile, PS Accessori Cell culture multiwell plates, treated, sterile, PS

• I filtri cellulari sono particolarmente adatti per la generazione di sospensioni omogenee unicellulari da tessuti o cellule primarie di lisati.
• Disponibili in 3 misure mesh 40 µm: 70 µm, and 100 µm
• Codifica colore delle misure mesh
• Studiato per adattarsi nelle provette da 50ml
• Confezionati individualmente
• Superficie manico e mantello per migliorata manipolazione asettica
• Nylon mesh
• Apirogeni

For research and development only.

Fiasche per colture cellulari con Nunclon™Δ. Le modifiche superficiali sono certificate apirogene e testate per la formazione di monostrati e l"efficienza della clonazione, con linee cellulari primarie e immortalate. Sterili. Collo fiasca ad angolo. Sterile.
Materiale fiasche: Polistirene
Materiale tappi: HDPE

Accessori Raschietto per cellule, sterile Accessori Caps for Cell Culture Flasks EasYFlask™, HDPE

Cell culture multiwell plates are available in the hydrophilic surface (TC surface treatment) for improved cell adhesion and hydrophobic surface for suspension cultures and hybridoma cells. All panels can be stacked together, both with and without cover. The special design of the panels is also in stacked plates a faster heat exchange over the entire bottom surface of each plate allows . All plates have an imprinted lot number and are both pyrogens and DNase / RNase - free .

Properties:

• High clarity and low autofluorescence
• Lid enables optimal gas exchange with the lowest possible evaporation
• Single position lids to prevent cross-contamination
• Alphanumeric well coding
• Compatible with common instruments and automated systems
• Easy-to-open packaging

Bottiglia in PC. Tappo a vite in PP. Graduata con bordo rinforzato. Autoclavabile. Ideale per stoccare soluzioni acquose e per conservazione per tempi brevi di campioni di colture cellulari.

These standard surface treated cell culture plates for the cultivation of adherent cells exhibit a reduced evaporation due to raised well rims and uniform rings on the lids.

• Disponibile con 4, 6, 12, 24, 48, 96, 384-pozzetti (coperchio incluso)
• Fondo pozzetto piatto
• Piastra a 96 pozzetti disponibile anche con fondo del pozzetto tondo (6.267 171)
• Posizione singola del coperchio che riduce i rischi di contaminazioni crociate
• I pozzetti sono etichettati con codice alfanumerico per una facile identificazione
• Sterilizzate a raggi gamma
• Apirogene
• Materiale: Polistirene

La camera del vetrino elimina la necessità del trasferimento di cellule prima della visualizzazione / colorazione. La struttura superiore può essere rimossa quando la coltura è completa. Utile per test virali e su micoplasma, studi su cromosomi, test di tossicità e immunocitologia. Sterile. La superficie di crescita rivestita chimicamente del Sistema Lab-Tek™II-CC² Chamber Slide imita la polilisina e fornisce siti di legame ottimali per cellule fastidiose (tipo neuroni).
Materiale della camera: Polistirene

Lab-Tek™: Sigillante, silicone non tossico, grado medicale
Lab-Tek II™: Sigillante, adesivo acrilico biocompatibile
Lab-Tek II™ - CC²: vetrino in vetro modificato, rivestito chimicamente da polilisina similare

Piastre multipozzetto da 4 a 48 pozzetti con superficie trattata Nunclon™Δ. Le piastre a 6 pozzetti sono disponibili anche con rivestimento Poly-D-Lysine e Collagen I.
I bordi sollevati dei pozzetti abbassano il rischio di contaminazioni incrociate.
Eccellente qualità ottica. Tutte le modificazioni superficiali disponibili sono certificate apirogene e testate sia per la formazione del monostrato che per l"efficienza di clonazione, impiegando cellule primarie così come ripetute linee di cellule. Sterili. Con coperchio
Materiale piastre e coperchio: Polistirene

Le fiasche per colture cellulari Nuclon™Δ con superficie da 25 a 175 cm²sono disponibili con tappo filtrante o tappo Vent/Close per ottimo scambio di gas. Corte, collo largo (angolato o diritto) consentono facile accesso all"intera superficie di crescita. Il trattamento Nuclon™Δ è limitato all"area di crescita. Le aree del collo non sono trattate per prevenire attaccamento e crescita delle cellule in aree non volute. Certificate apirogene e testate per formazione di monostrato ed efficienza di clonazione impiegando cellule primarie così come linee di cellule. Sterili.
Materiale piastre: Polistirene
Materiale tappi: HDPE

Accessori Tappi per bottiglie Nunclon™ Δ, HDPE Accessori Raschietto per cellule, sterile Alternativa Fiasche per Coltura Cellulare EasYFlask™, trattate, PS/HDPE, sterili

Piastre chiare, bianche o nere con eccellente chiarezza ottica e pozzetti a fondo piatto. Modificazioni della superficie Nunclon™Δ, Collagene I, Poly-D-Lisina adatte per test di culture cellulari.
Materiale piastre: Polistirene
Volume totale µl/pozzetto: 400

With blue vented closure, the chance for cross contamination is reduced. Ideal for shaker and suspension cell culture, media preparation or storage. Made of light, crystal clear PETG. Molded-in graduations. Leak-proof HDPE closure has a hydrophobic 0.2 µm PTFE membrane that allows sterile air exchange. Flasks offer a 5-year shelf life, a 10^-6 SAL, are non-pyrogenic and non-cytotoxic. Individually packaged for easy storage and handling.

Accessori Vented closure, HDPE, for disposable Erlenmeyer flasks Nalgene™ Alternativa Disposable Erlenmeyer Flasks Nalgene™ with screw closure, PETG, sterile, with flat bottom Alternativa Beute Erlenmeyer Nalgene™, Tipo 4112, 4113, PETG, sterili

In the case of concentration determinations in microplates, the measurement is made vertically and the layer thickness of the sample to be measured is dependent on the sample volume. Even with smaller sample volumes, the resulting layer thicknesses are sufficient for precise measurement. At a constant sample volume, concentrations can be determined with the aid of a calibration curve.

Properties:

• Optical window down to 230 nm ideal for nucleic acid determinations at 260 nm/280 nm
• For measurements of protein concentration at 280 nm
• Transmission measurements with UV light up to 230 nm
• DMSO-resistant
• Stored at -20 °C without any problem

Densitometro da banco efficiente e compatto per misurare la torbidità di sospensioni cellulari in diverse applicazioni di life science. I densitometri sono progettati e calibrati dal produttore per misurare la torbidità nel range da 0.3 a >7.5 unità McFarland (DEN-1) e da 0.00 a >15.00 unità McFarland (DEN-1B) con una piccola deviazione standard.

Caratteristiche
Sorgente luminosa: Lunghezza d'onda:	diodo 565nm ±15nm
Range di misura DEN-1: Range di misura DEN-1B:	da 0.3 a >7.5 McF da 0.0 a >15.00 McF
Precisione:	±3%
Tempo di misura:	1 sec
Diametro tubi, esterno: Diametro tubo con adatttaore D16 (incluso):	18mm 16mm
Volume campione: Dimensioni (L x P x H):	≥2ml 165 x 115 x 75mm
Alimentazione:	12V (DEN-1B anche 3 batterie AAA)

Accessori Accessories for Densitometer DEN-1 / DEN-1B

For cell culture, Greiner Bio-One offers standard and filter cap cell culture flasks with growth areas of four different sizes. Suspension culture flasks complete the range in another sizes.They are made of high-grade polystyrene. The special design of our cell culture flasks makes it possible to efficiently reach the cell lawn with a cell scraper, whilst still providing optimal access with a pipette. The special neck
design reduces the risk of wetting the inside of the cap with medium, thus providing additional protection against contamination. The stacking rim on the top of the flasks ensures firm standing and easy stackability in the incubator.

Properties:

• Improved cell adhesion through physical surface treatment
• Cell culture flasks with filter screw cap
• Canted neck
• Graduation on both sides
• Sterile and user-friendly packaging
• 25 cm², 75 cm² and 175 cm² growth areas
• High and flat design of the 175 cm² cell culture flask for variability of media volume

Ottimizzazione formazione sferoide

• Permette alle cellule di crescere in sospensione praticamente senza adesione cellulare
• Supporta diversi tipi di cellule e la loro capacità di generare sferoidi
• Consente, la crescita costante e ripetibile delle cellule

Crescita culture cancerose 3D

• Minimizza la variabilità e sostiene la formazione costante di sferoidi tumorali che simulano le strutture 3D di crescita tumorale, fornendo un migliore sistema modello per studiare la progressione delle cellule tumorali e l"efficacia di farmaci antitumorali in vitro

Formazione Promozione di corpi embrionali (EB)

• Dimostra qualità superiore per la formazione del corpo embrioide di cellule staminali pluripotenti con una minima differenziazione spontanea
• Le cellule risultanti sono in grado di differenziarsi in tutti e tre gli strati embrionali

Certificazioni / Compliance

• Gratuita di prodotti di origine animale
• Apirogeno: Endotossina testati (0,5 EU / mL)
• Biologicamente inerte: USP reattività biologica Classe VI
• Alto livello di sterilità: SAL 10^-6, sterilizzazione ossido di etilene
• Validità 4 anni

Alternativa Multidish Nunclon™ Sphera™ Alternativa Cell culture dishes Nunclon™ Sphera™

96 Well Polystyrene Microplates are manufactured under DIN ISO 9001 guidelines and can be traced all the way back to production through a defined LOT number system. Furthermore they are footprint compatible with automated systems. Polystyrene is a highly clear polymer with excellent optical properties which makes it ideal for precise optical measurements. An it is suitable for work with cell cultures.

Properties:

• F-Bottom / Chimney Well: chimney-like arrangement wells, each well stands on its own
• FLUOTRAC™ are black microplates for fluorescence measurements
• LUMITRAC™ are white microplates for luminescence measurements
• medium binding microplate is more hydrophobic than the surface of a high binding microplate
• Are analysed for detectable DNase, RNase and human DNA
• Are free of biocides and antistatics
• Are manufactured without the use of siliconbased mould release