Protein-blotting

Supported Nitrocellulose transfer membranes combine the binding characteristics of nitrocellulose membranes with the strength of nylon membranes. These membranes are used for DNA/RNA transfers requiring low background or for multiple hybridizations. For use with chemiluminescence and radiographic detection systems. BSA binding capacity up to 100 µg/cm².

The safety data sheets of these products are available under www.llg-labware.com.

Nitrocellulose membranes with a 0.2 µm pore size, offering excellent sensitivity, resolution and low background for Western blot protocols.

Features & Benefits:

• Low background and excellent signal-to-noise ratios, without the need for stringent washing conditions
• Compatible with a variety of Western blot labeling and detection systems
• Risk of ”blow-through“ reduced by high binding capacity of small proteins (< Mr 20 000)
• Optimized for chemiluminescent and fluorescent detection
• Long shelf life of bound proteins to the transfer membrane

Double-coated, high sensitivity film optimized for use in all blue and green light chemiluminescence systems for Western blot analysis and validated for use with Amersham ECL™ and CDP-Star.

Features & Benefits:

• High sensitivity Western blot membrane to detect low concentrations of nucleic acid and protein targets
• Clear background for excellent contrast and improved band resolution
• Produces publication-quality images
• Easy to read and simple-to-use
• To be processed in automatic processors or manually in tanks and trays using most common X-ray film developers and fixers

PVDF blotting membrane with 0.45 µm pore size, hydrophobic and of high physical strength, for use with standard colorimetric and chemiluminescent detection methods for proteins of < Mr 20 000.

Features & Benefits:

• Protein binding capacity over 200 μg/cm² for easy signal detection.
• High protein retention even after harsh washing steps.
• High protein capture during transfer minimizes sample loss.
• Suitable for stripping and reprobing

Low fluorescent, hydrophobic blotting membrane for protein transfer. Optimized for fluorescence detection in Western blotting applications.

• Protein binding capacity over 200 µg/cm²
• Higher sensitivity due to low background fluorescence
• Compatible with many blocking agents, transfer buffers and total protein blot stains
• Available in the standard precut sizes for laboratory applications

PVDF blotting membrane with 0.2 µm pore size, hydrophobic with high physical strength, for use with standard colorimetric and chemiluminescent detection methods. The small pore size of the membrane minimizes "blow-through" and increases protein binding over a wide range of molecular weights.

Features & Benefits:

• High protein binding capacity for easy signal detection
• High protein retention even after harsh washing steps
• Maximum capture of proteins during transfers minimizes sample loss
• 0.2 μm pore size for higher surface area, resulting in better binding of low molecular weight proteins
• Highly suitable for stripping and reprobing

Hydrophobic Western blotting membrane specifically designed for protein sequencing applications and solid-phase amino acid analysis.

• High protein binding capacity
• Chemical resistance needed for N-terminal sequencing
• High protein retention even after harsh washing steps
• Minimizes "blow-through" and increases protein binding
• Available in popular sizes for laboratory application