DNA- og RNA-isolation

For rapid and efficient extraction of plasmid DNA from bacterial cells.
The method is based on a combination of alkaline lysis and RNase treatment to obtain a clear lysate with minimal genomic DNA and RNA contamination. The bound plasmid DNA is washed and finally eluted by adding a buffer. The test kit contains the I-Blue Lysis Buffer, an optional colour indicator. The use of this indicator prevents common handling errors that impair efficient cell lysis and neutralization.

• Fast extraction time
• High yield
• Prevention of handling errors during cell lysis and neutralisation by I-Blue colour indicator

The ready-to-use DNA markers run precisely and produce a sharp, well defined ladder. They are available in six molecular weight ranges. The markers contain high intensity reference bands and may be used to perform size comparisons with DNA molecules ranging from the smallest of PCR fragments to large, linearised cosmid vectors.

• Up to 19 bands
• Isolated from restrictive plasmids
• 6 months stable at room temperature
• 12 months shelf life at 4 °C
• 24 months storable at -20 °C
• Vials of 500 µl

Riplate^® magnetic plates have been specially developed for the reproducible magnetic separation of DNA and proteins and can also be used for cleaning, mixing or centrifugation of samples as well as for the storage and preparation of substances.
For the filtration of proteins as well as for DNA extraction, an automated method called magnetic separation is commonly used. The technology is based on magnetic particles which allow the fast and efficient sample preparation with high throughput. The sustainability of these products as well as reliable analysis results are ensured by the production under cleanroom conditions (class 8). The plates are in line with the SBS standard (Society for Biomolecular Screening) and have been especially developed for the magnetic separation technology.

• High resistance towards commonly used chemicals, solvents and alcohols
• High stability also during centrifugation
• Newly developed cylindrical wells enable a good bioturbation of samples
• Slightly raised rims reduce the risk of cross-contamination
• The alphanumeric code allows the registration and identification of individual samples even in confined spaces

Rack suitable for all magnetic separation experiments involving affinity ligands separation of e.g. antibodies and nucleic acids.

• Model for 1.5 / 2.0 ml, 5 ml / 15 ml and 50 ml tubes
• Independent and rotatable sleeves with powerful magnets on 50 ml and 1.5/5 ml
• Easy removal of liquids
• 96-well PCR plate model has 24 magnets
• Flat bottom microplate model has magnets covering entire footprint to accept all standard micro-plates with any number of wells; 6, 12, 24, 48, 96 etc.
• Racks fit microplates following ANSI standards
• Clear acrylic allows unobstructed view of tube contents

Each spin column contains 2 filters and is either recommended for purification of DNA (GF-F2) or RNA (GF-N2). The system consisting of 0.8 ml filter tube and 1.5 ml or 2.0 ml receiver tube.

• Recommended for purification
• Prefiltering of dirty solutions
• Rapid flow rate
• High particle loading capacity

Guanidine thiocyanate is a strong protein denaturant used in connection with the isolation of DNA and RNA.

Features & Advantages:

• free from DNases, RNases and proteases
• Content (titr.): min. 99 %.
• pH (1 M): 4.7 - 7.0

Magnetic beads kit for isolation of viral RNA and DNA from cell-free biological fluids. Suitable for automated use.

Flexible magnetic bead based kit for the isolation of DNA and RNA from water and air samples. Suitable for automated use.

Kit with magnetic beads for isolation of viral nucleic acids and bacterial DNA from clinical samples. Suitable for automated use.

The NucleoSpin™ Dx Virus Kit for isolation of viral nucleic acids from human serum or plasma for subsequent in vitro diagnostic investigations. The isolated and purified nucleic acids can be used in qualitative applications (e.g. RT-PCR) as well as in quantitative applications (e.g. detection of viral load by qPCR).

Features & Advantages:

• Suitable for a wide range of viruses, including: Corona viruses, HBV, HCV, HIV, CMV, H1N1
• Viral RNA from 150 µl human serum or plasma
• Useable samples: fresh or frozen plasma stabilized with EDTA or citrate, serum from commercially available blood collection systems

Mini kit for RNA purification with DNA removal column. Ultrafast and convenient RNA isolation without rDNase digestion.

The Nucleospin™ RNA Virus Kit is a reliable and safe method for the isolation of viral RNA, free from contaminants. Isolate viral RNA in only 30 minutes, thanks to the fast and convenient protocol. The kit contains the carrier RNA for highest sensitivity in downstream processes.

Features & Benefits:

• Suitable for a wide range of viruses, including: Corona viruses, influenza viruses, HCV, HIV, avian influenza virus, bluetongue virus
• Viral RNA from 150 µl serum, plasma and cell-free biological fluids
• downstream processes: RT-PCR, enzymatic reactions

Mini kit for viral RNA/DNA purification, allows time saving parallel isolation of viral RNA / ?DNA from different samples.