Blot Imaging

Highly sensitive chemiluminescence detection reagent with long-lasting signal for accurate detection of minute changes in protein levels. Stable signal emission, enabling repeated exposures and making it easier to process several blots in the same experimental run.

Features & Benefits:

• Intense and long-lasting signal allows for multiple exposures, making the reagent highly suitable for large experimental series and allowing for convenient handling time between the end of the experiment and detection
• Accurate detection of both high and low abundant proteins on the same blot after a single exposure
• Optimized for use with Amersham Hybond P (PVDF) membranes and compatible with Amersham Protran (nitrocellulose) Western blotting membranes
• Compatible with Amersham ECL Rainbow Molecular Weight Markers and Amersham ECL DualVue Western Blotting Markers

Ready-to-load marker providing sharper, more intense bands on gels and blots and improved band spacing for more accurate molecular weight determination. The bright, distinctive colors of the markers allow easier confirmation of transfer to blotting membranes and orientation.
Comprising a mixture of individually colored proteins that are combined to produce bands of equal color intensity. The approximate protein concentration is 1 to 2 mg/ml, depending on the product. Each 250 µl pack size is sufficient for use with 50 minigels (10 x 8 cm) or 25 large gels (20 x 20 cm).

Features & Benefits:

• Enhanced to enable faster and simpler identification of proteins
• Monitor progress of protein electrophoresis and assess transfer efficiency and molecular weight of blotted proteins without staining
• High intensity, pre-stained multicolored bands allow distinction of closely-related proteins
• For use on SDS-PAGE

Available formats:

• Full-range rainbow markers (Mr 12 000 to 225 000) use ten separate proteins with six different colors
• High-range rainbow markers (Mr 12 000 to 225 000) use eight separate proteins with six different colors
• Low-range rainbow markers (Mr 3 500 to 38 000) use seven separate proteins with five different colors

Complete solution for chemiluminescence and fluorescent Western blot imaging. With highly sensitive cooled CCD Camera with high quantum efficiency, for detection of picogram level proteins. Peltier cooling allows exposure times to be increased to detect faint chemiluminescence without adding annoying background noise. Suitable for chemiluminescence Western blots, auto-rads, DNA or RNA stained with ethidium bromide, SYPRO, SYBR and SAFE dyes on agarose gels, Coomassie blue or silver stained proteins on acrylamide gels, stain-free gels and fluorescent gels. Furthermore GFP-plantimaging, colonies or plaques detection on Agar plates, bioluminescence and 2d gels. The free of charge software allows automated imaging of multicolour blots with a simple and user friendly interface.

• Cooled, high resolution, high quantum efficiency (QE) camera
• Motor driven lens and 7-position motor-driven filter wheel
• Long-life white LED EPI lighting
• CFR21 Part 11 compliant
• With acquisition and analysis software genePIX and geneQUANT

Scope of supply:
Darkroom, camera, UV transilluminator, UV emission filter, license free acquisition and analysis software

Specifications
Resolution:	4 megapixels
Effective resolution:	16 megapixels
Lens:	Zoom, f1.2
Camera cooling:	-57 °C
Sensor bit-depth:	16 bit
Greyscales:	65536
Dynamic range:	4.8
Transilluminator wavelength:	302 nm
Transilluminator size:	250 x 300 mm
Dimensions (W x D x H):	570 x 450 x 840 mm
Weight:	37 kg
Power supply:	230 V/50 Hz

Accessories Filters for Gel documentation systems chemiPRO Accessories HI-LED lighting modules for Gel documentation systems chemiPRO Accessories Converters for Gel documentation systems chemiPRO and gelPRO Accessories Transilluminator for Gel documentation systems chemiPRO and gelPRO Accessories UV blocking shield for Gel documentation systems chemiPRO and gelPRO Accessories Thermal printer P95DW for Gel documentation systems Alternative Mini Western Blotting Workflow Package WESTERNFLOWPROMINI

Imaging system for the detection of proteins in Western Blot analysis. Peltier cooling of the highly sensitive CCD camera allows exposure times to be increased to detect even faint chemiluminescence without adding background noise.

• Cooled, high-resolution camera with high quantum efficiency (QE)
• Motorised lens, stage and filter wheel (7-stage)
• Long-life white LED EPI lighting
• CFR21 Part 11 compliant
• With acquisition and analysis software genePIX and geneQUANT

Scope of supply: Gel documentation system, UV filter, manual, acquisition and analysis software

Lense:	f0.8 or f0.95, auto focus
Camera cooling:	-57 °C
Sensor bit-depth:	16 Bit
Greyscales:	65536
Dynamic range:	4.8
Max. image area:	345 x 276 mm
Dimensions (W x D x H):	570 x 550 x 990 mm
Weight:	45 kg
Power supply:	230 V, 50 Hz

Accessories Filters for Gel documentation systems chemiPRO Accessories HI-LED lighting modules for Gel documentation systems chemiPRO Accessories Converters for Gel documentation systems chemiPRO and gelPRO Accessories Transilluminator for Gel documentation systems chemiPRO and gelPRO Accessories UV blocking shield for Gel documentation systems chemiPRO and gelPRO Accessories Thermal printer P95DW for Gel documentation systems

Very sensitive chemiluminescent detection reagent with highly intense signal output, for detection of medium to very low protein levels. High signal intensity makes ECL Select suitable for demanding Western blotting applications, allowing detection of minute protein quantities. Its long lasting signals allows for multiple exposures and a convenient time window between the experiment and analysis. Signals can be detected with a CCD camera or X-ray film.

Features & Benefits:

• Exceptional signal intensity resulting in brighter, clearer bands at low protein levels.
• Delivering high sensitivity even when using highly diluted primary and secondary antibodies.
• Optimized for use with Amersham Hybond P (PVDF) membranes and compatible with Amersham Protran (nitrocellulose) membranes.
• Compatible with Amersham ECL Molecular Weight Rainbow Markers and Amersham ECL DualVue Western Blotting Markers.

The Immobilon Western HRP substrate offers a high sensitivity for Western or dot/slot/spot blotting on PVDF membranes as well as on nitrocellulose membranes. The HRP substrate is compatible with all commonly used buffers and blocking reagents. Blots on PVDF membranes can be re-examined, allowing detection of multiple target proteins on the same blot.

ECL substrate with stable light output. The formulation provides a low background for a high signal to noise ratio.
Test kit includes luminol / enhancer and peroxide solutions.

LumiGO: For low picogram detection. 125 ml luminol/enhancer solution, 125 ml peroxide solution.

LumiPRO: For low femtogram detection and an very high signal intensity. 50 ml luminol/enhancer solution, 50 ml peroxide solution.

• Long signal duration
• Working solution stable for at least three days

Complete package for Western blotting of mini gels with chemiluminescence. It includes everything necessary from gel loading to gel documentation and quantification.

• Gel documentation system chemiPro with UV transilluminator and large imaging area. Acquisition and analysis software is already included licence-free
• Vertical electrophoresis tank omniPAGE Mini for protein analysis in up to 4 gels
• Power supply PowerPro can be operated in constant mode or programmed. With the power supply can be used to operate both the electrophoresis tank and the semi-dry blotter

Scope of supply: Gel documentation system chemiPro-302E, electrophoresis tank omniPAGE Mini SYS, power supply PowerPro 3AMP, semi-dry blotter SD20, roller for western blotting, blotting paper (10 x 10 cm), PVDF transfer membrane (roll á 28 cm x 3 m, 0.22 µm pore size), protein ladder BLUE Wide Range (10 to 245 kDa), ECL substrate kit LumiPRO, Blocking solution (500 ml), Tris-Glycine SDS buffer (1 l)

Complete package for Western blotting of mini gels with chemiluminescence. It includes everything necessary from gel loading to gel documentation and quantification.

• Gel documentation system chemiLITE with Peltier-cooled CCD camera. Acquisition and analysis software is already included licence-free
• Vertical electrophoresis tank omniPAGE Mini for protein analysis in up to 4 gels
• Power supply PowerPro can be operated in constant mode or programmed. With the power supply can be used to operate both the electrophoresis tank and the semi-dry blotter

Scope of supply: Gel documentation system chemiLITE, electrophoresis tank omniPAGE Mini SYS, power supply PowerPro 3AMP, semi-dry blotter SD10, roller for western blotting, blotting paper (10 x 10 cm), PVDF transfer membrane (roll á 28 cm x 3 m, 0.22 µm pore size), protein ladder BLUE Wide Range (10 to 245 kDa), ECL substrate kit LumiPRO, Blocking solution (500 ml), Tris-Glycine SDS buffer (1 l)