Protein blotting

Le membrane di trasferimento supportate da nitrocellulosa combinano le capacità leganti delle membrane in nitrocellulosa con la forza delle membrane in nylon. Queste membrane sono utilizzate per trasferimenti di DNA/RNA che richiedono basso background o per ibridizzazioni multiple. Per uso con sistemi di rilevamento in chemiluminescenza e radiografici.
Affinità legante BSA fino a 100 µg/cm².

Le schede di sicurezza di questi prodotti sono disponibili su www.llg-labware.com.

Nitrocellulose membranes with a 0.2 µm pore size, offering excellent sensitivity, resolution and low background for Western blot protocols.

Features & Benefits:

• Low background and excellent signal-to-noise ratios, without the need for stringent washing conditions
• Compatible with a variety of Western blot labeling and detection systems
• Risk of ”blow-through“ reduced by high binding capacity of small proteins (< Mr 20 000)
• Optimized for chemiluminescent and fluorescent detection
• Long shelf life of bound proteins to the transfer membrane

Double-coated, high sensitivity film optimized for use in all blue and green light chemiluminescence systems for Western blot analysis and validated for use with Amersham ECL™ and CDP-Star.

Features & Benefits:

• High sensitivity Western blot membrane to detect low concentrations of nucleic acid and protein targets
• Clear background for excellent contrast and improved band resolution
• Produces publication-quality images
• Easy to read and simple-to-use
• To be processed in automatic processors or manually in tanks and trays using most common X-ray film developers and fixers

Nitrocellulose membranes with a 0.45 µm pore size for use in Western blot protocols. Excellent sensitivity, resolution, and low background. for a wide range of laboratory applications.

Features & Benefits:

• Low background and excellent signal-to-noise ratios
• Compatible with a variety of labelling and detection systems
• High binding capacity
• Suitable for proteins with a wide range of molecular weights
• Ideal tool for your antibody purification and analysis workflow
• Long shelf life of bound proteins

PVDF blotting membrane with 0.45 µm pore size, hydrophobic and of high physical strength, for use with standard colorimetric and chemiluminescent detection methods for proteins of < Mr 20 000.

Features & Benefits:

• Protein binding capacity over 200 μg/cm² for easy signal detection.
• High protein retention even after harsh washing steps.
• High protein capture during transfer minimizes sample loss.
• Suitable for stripping and reprobing

Low fluorescent, hydrophobic blotting membrane for protein transfer. Optimized for fluorescence detection in Western blotting applications.

• Protein binding capacity over 200 µg/cm²
• Higher sensitivity due to low background fluorescence
• Compatible with many blocking agents, transfer buffers and total protein blot stains
• Available in the standard precut sizes for laboratory applications

PVDF blotting membrane with 0.2 µm pore size, hydrophobic with high physical strength, for use with standard colorimetric and chemiluminescent detection methods. The small pore size of the membrane minimizes "blow-through" and increases protein binding over a wide range of molecular weights.

Features & Benefits:

• High protein binding capacity for easy signal detection
• High protein retention even after harsh washing steps
• Maximum capture of proteins during transfers minimizes sample loss
• 0.2 μm pore size for higher surface area, resulting in better binding of low molecular weight proteins
• Highly suitable for stripping and reprobing