Protein analizi

Media for bacteria
Luria Bertani (Miller):

• Standard high salt content media for E. coli cultivation
• Ideal for plasmid preparation

Luria Bertani (Lennox):

• Standard medium with low salt content for the cultivation of E. coli
• Ideal for cultivating recombinant E.coli strains

Terrific Broth:

• Rich content media for the high density cultivation of E. coli
• Ideal for protein expression and plasmid preparation

2xYT Broth:

• Nutritive medium for the cultivation of recombinant E. coli strains and for the growth of filamentous bacteriophages

Agar
Bact. Agar:

• High quality agar used for the preparation of culture media and other bacteriological applications

Luria Bertani Agar (Miller):

• Standard Agar based on Luria Bertani (Miller) media with high salt content
• Ideal for growth and maintaining especially of E. coli strains used in molecular microbiology procedures

Luria Bertani Agar (Lennox):

• Standard Agar based on Luria Bertani (Lennox) media with low salt content
• Ideal for growth and maintaining especially of recombinant E. coli strains

Alicyclobacillus Detection Agar:

• Agar for the cultivation of Aliclyciclobacillus spp. in fruit juices

Orange Serum Agar:

• Agar for isolation, cultivation and enumeration of acid-tolerant microorganisms

PCA Agar:

• Non-selective agar for the cultivation and standardized enumeration of aerobic microorganisms

Media for yeasts
YPD-Broth:

• Standard medium for the cultivation of Saccharomyces cerevisiae and other yeasts

Media components
Yeast Extract:

• Concentrate of the water-soluble portion of autolyzed Saccharomyces cerevisiae cells
• Non-animal product, used widely for non-animal formulations of bacterial, fungal, mammalian and insect cell culture

Tryptone:

• Pancreatic digest, tryptone from casein
• Excellent nutrient for use in culture media for producing antibiotics, toxins, enzymes and other biological products

Casein Peptone:

• Pancreatic digest, peptone from casein
• Ideal for use in fermentation applications and microbiological culture media

For numerous micro- and molecular biological applications.
The safety data sheets of these products are available under www.llg-labware.com.

In contrast to nitrocellulose, Immobilon membranes have a number of advantages. An optimized coloring capacity, a broad solvent compatibility and a lower background make the membrane their solution. Immobilon membranes do not rupture or curl and can be cut without breaking. Reprobing is also possible many times. The Immobilon P membrane is particularly suitable for proteins with a molecular weight greater than 20 kDa.

Features & Advantages

• 0.45 µm Pore size
• Suitable for Western Blotting
• Millipore Rapid Immunodetection Protocol reduces detection time by up to 2 hours

For sterilisation of aqueous solutions. The filter units are suitable for use with syringes, peristaltic pumps and pressure vessels.

• Sterile
• Hydrophilic
• Low protein binding
• Luer-Lock inside

These cards are specialty product for specific use cases. For standard sample collection and storage, it is advised to use the GenSaver 2.0 cards.

• AutoCollect: Cards suitable for automated processing, available as color variant for clear samples or with a barcode
• BioSample: These cards are especially suitable if you're not only interested in the nucleic acids but also in other biomolecules like proteins. Optionally with perforated sample spots, or IVD certification
• Sample cards for IVD use, optionally with perforated spots

The marker is covalently bound to a blue colour chromaphore to produce a ladder of evenly interspersed bands of uniform intensity. The coloured reference bands are used as visual indicators for the process of gel electrophoresis and the efficiency of the transfer to membranes in the Western Blot. The marker is suitable for the precise identification of the molecular weight of most cellular proteins.

• Easy identification in gels and blots
• Detectable from 2.5 µl
• 2 years stable at - 20 °C
• Vials of 500 µl

Modules in standard 96-well frames. The white and black immuno breakable modules are specially designed for fluorometric and luminometric immunoassays. Each well snaps into the frame and maintains the same height for accurate reading and efficient washing. Black plates for fluorescence measurements, white plates for luminescence detection.

• Bottom C-shaped
• Standard format 128 x 86 mm
• High optical quality for reliable results
• Free-standing modules with easy handling
• Alphanumeric system for better orientation

Choice of different surfaces:
PolySorp™, Immulon™ 1B and Universal Binding (UB) serve to absorb hydrophobic molecules.
MaxiSorp™, Immulon 4 HBX and Enhanced Binding (EB) are hydrophilic and ideal for antibody sandwich assays.

With fixed nozzle from stainless steel or aluminium.

The Immobilon Western HRP substrate offers a high sensitivity for Western or dot/slot/spot blotting on PVDF membranes as well as on nitrocellulose membranes. The HRP substrate is compatible with all commonly used buffers and blocking reagents. Blots on PVDF membranes can be re-examined, allowing detection of multiple target proteins on the same blot.

Durapore® PVDF membranes are characterized by high throughput and high flow rate. To maximize recovery and minimize interaction with your sample, the membrane has very low protein binding.

Features & Benefits:

• Chemical compatibility
• Automation compatible
• Suitable for: Receptor/Ligand Binding Assay, Protein Kinase/Phosphatase Precipitation Assays, Bead-Based Assay

If you want to have free chlorine, large particles and organic compounds removed from your tap water, use the Progard® pre-treatment packs. The reverse osmosis membrane (RO) is protected from chlorine oxidation, mineral scaling and organic pollution.

Features & Benefits:

• Ensures optimization of system life and performance

Silver-impregnated activated carbon for free chlorine reduction and bacteriostatic protection

• Depth filtration for particle removal

Amicon® ultra centrifugal filters promote high sample recovery, even in diluted samples, through ultrafiltration and also provide fast sample processing. Particularly suitable for sensitive downstream applications as the centrifugal filters provide the fastest and most efficient concentration. The Ultracel-3 centrifugal filters have a NMWL (Nominal Molecular Weight Limit) of 3 kDa.

Features & Benefits:

• Prevents turning to dryness
• Provides a predictable concentration factor
• No calibration is necessary to run multiple samples in parallel
• Vertical membrane aligned with filtrate instead of vertical for loss constipation, less waste and faster filtration
• 25 to 80 times the concentration in one step
• Wide chemical compatibility, heat-sealed membrane and compatible with pH 1-9
• Maximum sample recovery as Ultracel® low-bund membrane > 90 % recovery
• Centrifuge your valuable sample with security in a robust, slim unit that prevents leakage

Solutions of any macromolecules, simple and efficient, desalt and concentrate. Suitable for any centrifuge holding 1.5 ml tubes.

Features & Benefits:

• Even with diluted solutions the recovery is more than 95%.
• Reverse spin to maximize recovery even in the smallest sample
• Storage of filtrate or concentrated sample, very easy in standard microcentrifuge tubes
• concentration factor up to 100 x