Kültür (biyoloji)

The cell culture flasks from LABSOLUTE® are made exclusively of PS, corresponding to the highest international standards (USP Class VI). A short, wide and angled neck allows an easy handling while the risk of contamination is minimized. The treatment of surfaces allows adherent cells a uniform and even cell adhesion.

• Short and wide sloping neck for easy access
• Easily stackable
• Both flasks sides have engraved graduation with a labelling area
• Screw cap with a hydrophobic 0.22 μm filter for ventilation
• Packed upright in resealable bags
• Surface is treated with vacuum gas plasma
• Sterilized by radiation (SAL 10^-6)

Accessories Cell scrapers and cell lifters, sterile Alternative Cell culture dishes, treated, sterile, PS Alternative Cell culture multiwell plates, treated, sterile, PS

The LABSOLUTE® filtration units are ideal for filtration of buffer solutions, cell culture media, microbiological media and other biological fluids. The PES membrane guarantees good flow through times and low protein binding.

• High traceability thanks to expiry date and Lot. no. on each filter
• 0.2 μm: Ideal for sterilization applications
• 0.45 μm: Ideal for clarifying buffer solutions and nutrient media preparation
• Filtrate bottle with lid for convenient storage of the filtered solution
• Individually packaged
• Different volumes available
• Pyrogen free and not cytotoxic
• Quick connection for hoses with an inside diameter of 8 to 12 mm
• Gamma sterilized (SAL 10^-6)

The complete filtration unit consists of a filter funnel with vacuum connection and lid, the filtrate bottle and the associated screw cap.

Accessories Filter funnel, sterile, PES Accessories Filtrate bottle, sterile, PS

The LABSOLUTE® multiwell tissue culture plates are suitable for handling multiple samples in a single experiment. From 4-well up to 96-well plates, we offer you the appropriate multiwell plate for every experiment. Numbered wells and a rounded corner in the cover reduce misplacement and facilitate identification. The treatment of surfaces allows adherent cells a uniform and even cell adhesion.

• Evaporation is reduced by condensation rings
• Easily stackable
• Individually wrapped in paper/plastic blister pack
• Surface is treated with vacuum gas plasma
• Sterilized by radiation (SAL 10^-6)

Accessories Cell scrapers and cell lifters, sterile Alternative Cell culture dishes, treated, sterile, PS Alternative Cell culture flasks, treated, sterile, PS

The LABSOLUTE® filtration units are ideal for filtration of buffer solutions, cell culture media, microbiological media and other biological fluids. The PES membrane guarantees good flow through times and low protein binding.

• High traceability thanks to expiry date and Lot. no. on each filter
• 0.2 μm: Ideal for sterilization applications
• 0.45 μm: Ideal for clarifying buffer solutions and nutrient media preparation
• Filtrate bottle with lid for convenient storage of the filtered solution
• Individually packaged
• Different volumes available
• Pyrogen free and not cytotoxic
• Quick connection for hoses with an inside diameter of 8 to 12 mm
• Gamma sterilized (SAL 10^-6)

LABSOLUTE® filtrate bottle with ergonomic design for storing sterile solutions made of PS with a PE cap.

Accessories Filtration units, sterile, PES Accessories Filter funnel, sterile, PES

The LABSOLUTE® cell strainers are made of a strong nylon mesh and are characterized by an even distribution of the mesh pores. With the sterile cell sieves, cell suspensions can be quickly and easily filtered to collect primary cells or prepare samples for flow cytometry.

• Available in three mesh sizes: 40, 70 and 100 μm
• Color coded for easy distinction
• Extended edge allows easy handling even with tweezers
• Designed to fit standard 50 ml centrifuge tubes
• DNase, RNase and pyrogen-free
• Individually packed, gamma-irradiated (SAL 10^-6)

Accessories Centrifuge tubes, PP

The LABSOLUTE® cell scrapers and lifters enable easy and efficient cell harvest. Optimize your cell harvest by using the cell scraper for cell culture flasks and cell lifter for dishes. The handle is made of ABS and allows the controlled use of the thin, flexible blade made of TPE.

• Scraper in two different handle lengths (25 cm with 2 cm blade and 39 cm with 3 cm blade)
• Two blade positions: scraper and lifter
• Individually wrapped in paper/plastic blister packs

Accessories Cell culture dishes, treated, sterile, PS Accessories Cell culture flasks, treated, sterile, PS Accessories Cell culture multiwell plates, treated, sterile, PS

The LABSOLUTE® filtration units are ideal for filtration of buffer solutions, cell culture media, microbiological media and other biological fluids. The PES membrane guarantees good flow through times and low protein binding.

• High traceability thanks to expiry date and Lot. no. on each filter
• 0.2 μm: Ideal for sterilization applications
• 0.45 μm: Ideal for clarifying buffer solutions and nutrient media preparation
• Filtrate bottle with lid for convenient storage of the filtered solution
• Individually packaged
• Different volumes available
• Pyrogen free and not cytotoxic
• Quick connection for hoses with an inside diameter of 8 to 12 mm
• Gamma sterilized (SAL 10^-6)

The filter funnels from LABSOLUTE® can be easily screwed onto all standard bottles with a 45 mm thread. The membrane type and pore size are imprinted for easy identification.

Accessories Filtration units, sterile, PES Accessories Filtrate bottle, sterile, PS

Cell strainers are especially suited for the generation of homogenous single cell suspensions from tissue or primary cell lysates.

• Colour coded mesh size
• Designed to fit into 50 ml tubes
• Individually packaged
• Handle and mantle surface for improved aseptic handling
• Nylon mesh
• Pyrogen-free

For research and development only.

For research and development only.

Cell culture multiwell plates are available in the hydrophilic surface (TC surface treatment) for improved cell adhesion and hydrophobic surface for suspension cultures and hybridoma cells. All panels can be stacked together, both with and without cover. The special design of the panels is also in stacked plates a faster heat exchange over the entire bottom surface of each plate allows . All plates have an imprinted lot number and are both pyrogens and DNase / RNase - free .

Properties:

• High clarity and low autofluorescence
• Lid enables optimal gas exchange with the lowest possible evaporation
• Single position lids to prevent cross-contamination
• Alphanumeric well coding
• Compatible with common instruments and automated systems
• Easy-to-open packaging

In the case of concentration determinations in microplates, the measurement is made vertically and the layer thickness of the sample to be measured is dependent on the sample volume. Even with smaller sample volumes, the resulting layer thicknesses are sufficient for precise measurement. At a constant sample volume, concentrations can be determined with the aid of a calibration curve.

Properties:

• Optical window down to 230 nm ideal for nucleic acid determinations at 260 nm/280 nm
• For measurements of protein concentration at 280 nm
• Transmission measurements with UV light up to 230 nm
• DMSO-resistant
• Stored at -20 °C without any problem

For cell culture, Greiner Bio-One offers standard and filter cap cell culture flasks with growth areas of four different sizes. Suspension culture flasks complete the range in another sizes.They are made of high-grade polystyrene. The special design of our cell culture flasks makes it possible to efficiently reach the cell lawn with a cell scraper, whilst still providing optimal access with a pipette. The special neck
design reduces the risk of wetting the inside of the cap with medium, thus providing additional protection against contamination. The stacking rim on the top of the flasks ensures firm standing and easy stackability in the incubator.

Properties:

• Improved cell adhesion through physical surface treatment
• Cell culture flasks with filter screw cap
• Canted neck
• Graduation on both sides
• Sterile and user-friendly packaging
• 25 cm², 75 cm² and 175 cm² growth areas
• High and flat design of the 175 cm² cell culture flask for variability of media volume

Cell culture multiwell plates are available in the hydrophilic surface (TC surface treatment) for improved cell adhesion and hydrophobic surface for suspension cultures and hybridoma cells. Cell culture microplates from the CELLSTAR® range are specifically treated. This treatment leads to polar groups such as carboxyl and hydroxyl groups , added to the plastic surface. These allow the growth of adherent cell lines. They are also suitable for automated systems and contain an alphanumeric well coding. They are also suitable for footprint systems and contain an alphanumeric well coding.

Properties:

• High clarity enables optimal microscopic examinations
• Lid enables optimal gas exchange with the lowest possible evaporation
• Stackable
• Alphanumeric well coding
• Individually wrapped peel-off bags
• Consecutive lot numbering
• 384 and 1536 well foramt available

96 Well Polystyrene Microplates are manufactured under DIN ISO 9001 guidelines and can be traced all the way back to production through a defined LOT number system. Furthermore they are footprint compatible with automated systems. Polystyrene is a highly clear polymer with excellent optical properties which makes it ideal for precise optical measurements. An it is suitable for work with cell cultures.

Properties:

• F-Bottom / Chimney Well: chimney-like arrangement wells, each well stands on its own
• FLUOTRAC™ are black microplates for fluorescence measurements
• LUMITRAC™ are white microplates for luminescence measurements
• medium binding microplate is more hydrophobic than the surface of a high binding microplate
• Are analysed for detectable DNase, RNase and human DNA
• Are free of biocides and antistatics
• Are manufactured without the use of siliconbased mould release

All plates consist of an optically clear borosilicate glass bottom. The glass bottom allows transmission measurements in the wavelength range above 350 nm. The 175 μm thick glass bottom of the SensoPlate™ is equivalent to the light path of standard coverslips. A adhesive is used for the fixing glass bottom plates to enable a minimal autofluorescence.

Properties:

• For the fluorescence correlation spectroscopy
• A light path of 175 +/- 15 μm
• available sterile with lid
• The footprint of all glass bottom microplates is conform to the ANSI 1-2004 standard

Combining a white or black upper structure with an optically clear, flat well bottom polymer base allows for reading from bottom and top, or both. Nunclon™Δ surface modifications are for cell culture optimized. Non-treated plates are ideal for scintillation counting.
Material plates: Polystyrene
Total volume µl/well: 120

Cell culture multiwell plates are available in the hydrophilic surface (TC surface treatment) for improved cell adhesion and hydrophobic surface for suspension cultures and hybridoma cells. Cell culture microplates from the CELLSTAR® range are specifically treated. This treatment leads to polar groups such as carboxyl and hydroxyl groups , added to the plastic surface. These allow the growth of adherent cell lines. They are also suitable for automated systems and contain an alphanumeric well coding. They are also suitable for footprint systems and contain an alphanumeric well coding.

Properties:

• Lid enables gas exchange with the lowest possible evaporation
• High clarity of the clear microplates for optimal microscopic examinations
• Stackable
• Alphanumeric well coding
• Consecutive lot numbering
• 96 and 1536 well format available

Radiation-sterilised. Working volume 10 to 100µl / well. Developed for large-scale DNA library manipulations. The standard microwell plate format of 128mm x 86 mm permits automation. C-shaped well bases provide good optical properties and good sample recovery. Space-saving. Replication system available. Compatible with robots. Also available in cell culture, as well as Collagen I, poly-D-lysin, MaxiSorp, Polysorp, Immulon 4HBX and non-binding qualities in white, black and transparent.

Material plates: Polystyrene
Total volume µl/well: 120

Microtiter plates for immunoassays with surfaces for affinity binding (passive streptavidin and immobilizer streptavidin, nickel chelate and glutathione, BioBind).

• Streptavidin-biotin interaction for a broad range of biomolecules
• Highly specific binding surface
• Improvement of the signal-to-noise ratio
• 96 well plate: Working volume 100 µl/well, total volume 350-400 µl/well
• 384 well plate: Working volume 50 µl/well, total volume 120 µl/well

Minimize plate evaporation through the uniquely engineered design of Thermo Scientific™ Nunc™ Edge-96 Well Plate. The edge plate is engineered for optical superiority allowing your automated imaging systems to operate efficiently. The plates are made of clear polystyrene for excellent optical properties making it ideal for optical measurements
Features:

• Reduced Edge Effect virtually eliminates evaporation in lengthy incubations
• Overall plate evaporation rate reduced to <2% after seven days of incubation
• Low evaporation permits full-plate results
• Large evaporative buffer zones eliminate well-to-well variability
• Perimeter moat can be filled with 1.7mL sterile H2O or media
• Low background fluorescence, ideal for optical instruments
• Ideal for image-based high content applications like measuring primary neuron cultures
• Customized bar coding available (upon request)

Minimize plate evaporation through the uniquely engineered design of Thermo Scientific™ Nunc™ Edge-96 Well Plate. The edge plate is engineered for optical superiority allowing your automated imaging systems to operate efficiently. The plates are made of clear polystyrene for excellent optical properties making it ideal for optical measurements
Features:

• Reduced Edge Effect virtually eliminates evaporation in lengthy incubations
• Overall plate evaporation rate reduced to <2% after seven days of incubation
• Low evaporation permits full-plate results
• Large evaporative buffer zones eliminate well-to-well variability
• Perimeter moat can be filled with 1.7mL sterile H2O or media
• Low background fluorescence, ideal for optical instruments
• Ideal for image-based high content applications like measuring primary neuron cultures
• Customized bar coding available (upon request)
• Untreated

Alternative 96-well plate Nunc™ Edge 2.0, with flat bottom, PS, with hydrophilic surface Nunclon™ D

Optimizing spheroid formation

• Allows cells to grow in suspension with virtually no cell attachment
• Supports many different cell types and their ability to generate spheroids
• Enables consistent, repeatable cell growth

Growing 3D cancer cultures

• Minimizes variability and supports the consistent formation of cancer spheroids that simulate the 3D structures of tumor growth, providing a better model system for studying tumor cell progression and efficacy of anticancer agents in vitro

Promoting formation of embryoid bodies (EB)

• Demonstrates superior quality for embryoid body formation of pluripotent stem cells with minimal spontaneous differentiation
• Resultant cells are able to differentiate into all three germ layers

Certifications/Compliance

• Free of animal products
• Pyrogen-free: Endotoxin tested (0.5 EU/mL)
• Biologically inert: USP biological reactivity class VI
• High level of sterility: SAL 10-6, ethylene oxide gas sterilization
• Shelf Life 4 years

Alternative Multidish Nunclon™ Sphera™ Alternative Cell culture dishes Nunclon™ Sphera™