Protein blotting

Supported Nitrocellulose transfer membranes combine the binding characteristics of nitrocellulose membranes with the strength of nylon membranes. These membranes are used for DNA/RNA transfers requiring low background or for multiple hybridizations. For use with chemiluminescence and radiographic detection systems. BSA binding capacity up to 100 µg/cm².

The safety data sheets of these products are available under www.llg-labware.com.

Nitrocellulose membranes with a 0.2 µm pore size, offering excellent sensitivity, resolution and low background for Western blot protocols.

Features & Benefits:

• Low background and excellent signal-to-noise ratios, without the need for stringent washing conditions
• Compatible with a variety of Western blot labeling and detection systems
• Risk of ”blow-through“ reduced by high binding capacity of small proteins (< Mr 20 000)
• Optimized for chemiluminescent and fluorescent detection
• Long shelf life of bound proteins to the transfer membrane

Nitrocellulose membranes with a 0.45 µm pore size for use in Western blot protocols. Excellent sensitivity, resolution, and low background. for a wide range of laboratory applications.

Features & Benefits:

• Low background and excellent signal-to-noise ratios
• Compatible with a variety of labelling and detection systems
• High binding capacity
• Suitable for proteins with a wide range of molecular weights
• Ideal tool for your antibody purification and analysis workflow
• Long shelf life of bound proteins

Recommended for blotting techniques with gels, like Southern, Northern and Western Blots, even Semidry-Blots of proteins

• Made from ultrapure cotton linters and cellulose, entirely without additives to ensure that no contamination will occur during the transfer steps
• Tested specifically for chromatographic and blotting techniques to ensure high and uniform capillary actions
• High wet strength for safe handling

Double-coated, high sensitivity film optimized for use in all blue and green light chemiluminescence systems for Western blot analysis and validated for use with Amersham ECL™ and CDP-Star.

Features & Benefits:

• High sensitivity Western blot membrane to detect low concentrations of nucleic acid and protein targets
• Clear background for excellent contrast and improved band resolution
• Produces publication-quality images
• Easy to read and simple-to-use
• To be processed in automatic processors or manually in tanks and trays using most common X-ray film developers and fixers

PVDF blotting membrane with 0.45 µm pore size, hydrophobic and of high physical strength, for use with standard colorimetric and chemiluminescent detection methods for proteins of < Mr 20 000.

Features & Benefits:

• Protein binding capacity over 200 μg/cm² for easy signal detection.
• High protein retention even after harsh washing steps.
• High protein capture during transfer minimizes sample loss.
• Suitable for stripping and reprobing

Nitrocellulose blotting membrane with 0.45 µm pore size, for excellent sensitivity, resolution and low background.

Features & Benefits:

• High binding capacity (162 - 180 µg IgG/cm²)
• Suitable for proteins of a wide range of molecular weights
• 100% pure nitrocellulose membrane for use with all ECL Western blotting systems and protein blotting applications.
• Available in popular precut sizes for laboratory application