SPE

Combination phase for SPE of hydrocarbons from water acc. to DIN H53 / ISO DIS 9377-4

Special combination phase of sodium sulphate and Florisil^®.

Recommended application: hydrocarbons from drinking, surface and waste waters.

Accessories Adapters for CHROMABOND® columns

Octadecyl modified silica phase for SPE, endcapped

Base material silica, pore size 60 Å, particle size 45 µm for C₁₈ ec, 100 µm for C₁₈ ec f (for fast flow), specific surface 500 m²/g, pH stability 2 to 8. Octadecyl phases, endcapped, carbon content 14 % very non-polar, hydrophobic interactions with a wide variety of organic compounds. Advantageous for clean-up of samples with large structural variations (polarity differences).

Recommended applications: non-polar compounds, aflatoxins, amphetamines, antibiotics, antiepileptics, barbiturates, caffeine, drugs, preservatives, fatty acids, nicotine, PAHs, pesticides, PCBs, heavy metals, vitamins. Very well suited for desalting of samples.

For simultaneous preparation of up to 12 or 24 samples,
replacement parts and accessories for special applications.

Vacuum manifold complete consists of: glass cabinet with lid and lid gasket, removable needles on lower side of lid, vacuum gauge, control valve, valves and caps, variable rack.

Accessories Accessories for chromabond vacuum chamber

Aminopropyl modified silica phase for SPE

Base material silica, pore size 60Å, particle size 45µm, specific surface 500m²/g, pH stability 2 to 8, aminopropyl phase, carbon content 3.5%, polar, weak anion exchanger.

Recommended applications: trace elements, lipids.

Combination phase for SPE analysis of PAH from water containing humic acids

Special combination phase: aminopropyl phase for removal of interfering humic acids, octadecyl phase for enrichment of PAH.

Recommended application: PAH from water containing humic acids.

QuEChERS method and pre-mixes
Within a few years after its devleopment by Anastassiades et al. the QuEChERS method has gained a leading position for determination of pesticide residues in food by GC-MS or LC-MS allowing rapid and cheap clean-up of strong matrix-contaminated samples.
Standard clean-up of food samples
10 g sample are homogenised with 10 ml acetonitrile. After adding the internal standard the sample is shaken with 4 g MgSO₄ and 1 g NaCI and afterwards centrifuged. 1 ml of the supernantant is spiked with 25 mg CHROMABOND^® Diamino and 150 mg MgSO₄ and shaken again. After centrifugation the supernatant is injected into the GC/MS.
Extraction mixtures for sample preparation for determination of pesticides in food samples.

MACHEREY-NAGEL offers a number of individually weighed and pre-mixed buffers and extraction mixtures, specially composed for different sample matrices.
Mix I and Mix II are extraction mixtures, Mix III to Mix VI are clean-up mixtures.

The food samples will be extracted with either Mix I or Mix II.
Afterwards they will be purified with one of the following mixtures:

• Mix III (samples with low fat content; e.g. apples, strawberries),
• Mix IV (moderate content of chlorophyll and carotinoids; e. g. carrots, lettuce),
• Mix V (high content of chlorophyll and carotinoids; e. g. bell peppers, spinach),
• Mix VI (high fat content; e. g. avocados).

Every mix is prepacked in a 15 ml centrifuge tube. Pack of 50 pieces.

Unmodified silica phase for SPE

Unmodified, weakly acidic silica, pore size 60Å, particle size 45µm, specific surface 500m²/g, pH stability 2 to 8, very polar, adsorbs humidity from air, for this reason it should be kept well closed and if necessary dried before use due to its high affinity for polar compounds it should not be conditioned with polar (e.g. methanol) or water-containing solvents.

Recommended applications: aflatoxins, chloramphenicol, pesticides, steroids, vitamins.

Extraction and clean-up mixtures for sample preparation in the pesticide analysis of vegetable foodstuffs according to EN 15662 using GC/MS or LC/MS after acetonitrile extraction and purification using the dispersive SPE-QuEChERS method.

The samples are first extracted with Mix A. Purification is done with one of the following mixtures:

• Mix B (Clean-up mixture for fruits and vegetables with low fat content, e.g. apples, strawberries)
• Mix C (Clean-up mixture for fruits and vegetables with high fat content, containing oil/wax, e.g. avocados)
• Mix D (Clean-up mixture for moderately chlorophyll/carotenoid-containing fruits and vegetables, e.g. carrots, salad)
• Mix E (Clean-up mixture for fruits and vegetables with high chlorophyll/carotenoid content, e.g. sweet peppers, spinach)

If required, add ceramic homogenising beads for better mixing.

• Mix A contains a bag with the required amount of mixture and a 50 ml empty tube
• Mix B, C, D and E are pre-weighed into a 15 ml tube

Octadecyl modified silica phase for SPE, not endcapped

Base material silica, pore size 60 Å, particle size 45 µm for C₁₈, specific surface 500 m²/g, pH stability 2 to 8 octadecyl phases, not endcapped, carbon content 14 % possesses more free silanols (SiOH), which allow secondary interactions with polar groups of the analytes.

Recommended applications: non-polar compounds, pesticides.

96-well polypropylene plates for simultaneous filtration of 96 samples
Advantages of this high-throughput system are:

• economical by saving time and solvent
• use of multi-channel pipettors facilitates liquid transfer steps
• readily adaptable to all common automated/robotic handling systems
• minimised dead volume (≤ 40µl)
• membrane materials correspond to the respective CHROMAFIL^® syringe filters

Empty CHROMABOND^® column (PP) for SPE, one PE-filter element is already inserted in the column.

Kieselguhr phase for liquid-liquid extraction

Base material coarse-grained kieselguhr, large pore size, high pore volume, constantly high batch-to-batch quality, pH working range 1 to 13.

Application: liquid-liquid extraction of highly viscous aqueous solutions such as physiological fluids (blood, plasma, and serum) in clinical chemistry, dyes in textiles, environmental and food analysis.

Glass columns availlable on request.

Polar modified polystyrene-divinylbenzene copolymer with a weak anion exchanger

Specific surface 650 to 700 m²/g, particle size 80µm, pore size 50 Å, pH stability 1 to 14. Due to bifunctional modification much more hydrophilic than conventional polystyrene-divinylbenzene polymers and thus easily wettable with water.

Recommended applications: polar herbicides / pesticides from water (acidic, neutral, basic), polar phenols from water, polyaromatic compounds, polychlorinated biphenyls, drug analysis from urine, blood, serum, plasma pharmaceuticals / active ingredients from tablets, creams.

Due to the bifunctional modification CHROMABOND^® Easy is considerably more hydrophilic than conventional polystyrene-divinylbenzene polymers and thus easily wettable with water. The Easy effect: Aqueous samples can be loaded directly without preconditioning! This means that little or even no conditioning is needed, in contrast to standard SPE materials, where recovery rates normally decrease, in the worst case down to zero! Depending on the separation task conditioning may be required and is recommended for method development. A positive side effect of the excellent wettability: there is no decrease of recovery rates, if the cartridge runs dry, therefore automation is easier or, in some cases compared to silica materials, only feasible with CHROMABOND^® Easy, because a permanent vacuum can be used without supervision.

Further advantages of using a polymeric material:

• high surface, this means very high binding capacity (2 - 5 times higher than silica-based adsorbents)
• less adsorbent is needed in the cartridge (without losing sensitivity or recovery)
• faster analysis, because the height of the adsorbent bed can be reduced
• acidic or basic solvents (e. g. TFA) do not destroy the phase, or lead to unintended side products

Because of the polar modification the material is suitable for a broad range of compounds (acidic, neutral, basic, polar and nonpolar substances). Highly reproducible recovery rates can be obtained, even if the cartridge runs dry (especially advantageous when using 96-well plates, where stopcocks are not available!)

Magnesium silicate for SPE

Matrix magnesium silicate (MgO to SiOH 15:85), high purity, particle size 150 to 250µm.

Recommended application: organic tin compounds, aliphatic carboxylic acids, PCB, PAH.

Spherical, hydrophobic polystyrene-divinylbenzene resin for SPE
Hydrophobic polystyrene-divinylbenzene copolymer pH stability 1 to14. High-purity material with highest reproducibility and lowest blank values due to a novel manufacturing process, spherical particles 85mm; pore size 55 to 60Å. Very high surface 1000m²/g; capacity 390mg/g (caffeine in water). Excellent recovery rates especially for the enrichment of pharmaceuticals/active ingredients due to the spherical structure of the particles, very homogeneous surface, and optimised pore structure.

Recommended applications: pharmaceuticals/active ingredients from tablets, creams and water/waste water, drugs and pharmaceuticals from urine, blood, serum and plasma trace analysis of pesticides.

QuEChERS method and pre-mixes
Within a few years after its devleopment by Anastassiades et al. the QuEChERS method has gained a leading position for determination of pesticide residues in food by GC-MS or LC-MS allowing rapid and cheap clean-up of strong matrix-contaminated samples.
Standard clean-up of food samples
10 g sample are homogenised with 10 ml acetonitrile. After adding the internal standard the sample is shaken with 4 g MgSO₄ and 1 g NaCI and afterwards centrifuged. 1 ml of the supernantant is spiked with 25 mg CHROMABOND^® Diamino and 150 mg MgSO₄ and shaken again. After centrifugation the supernatant is injected into the GC/MS.
Empty PP centrifuge tube with screw cap, 50 ml, without QuEchERS mixtures.

Special silica phase for SPE of THC and derivatives acidic analytes from biological fluids (urine, blood ...)

base material silica, pore size 60Å, particle size 45µm, specific surface 500m²/g, pH stability 2 to 8, special bifunctional modification - C₈/ SB (strong anion exchanger - quaternary amine).

Recommended applications: extraction of THC and derivatives from urine, blood, serum, plasma, acidic analytes from biological fluids.

aluminium oxide, acidic, neutral, basic

aluminium oxide, high purity, pore volume 0.90ml/g, particle size 60 to 150µm, specific surface 150m²/g, acidic, pH 4 ± 0.5, recommended application: together with phase SA for PCB and pesticides.

Alox A: acidic, pH 4 ± 0.5
Alox N: neutral, pH 7 ± 0.5
Alox B: basic, pH 9.5 ± 0.5

SPE-columns.

Octadecyl modified silica phase for SPE of polar analytes

Base material silica, pore size 60Å, particle size 45µm, specific surface 500m²/g, pH stability 2 to 8, special octadecyl phase for polar analytes, not endcapped, carbon content 15%.

Recommended applications: more polar compounds like pesticides and their polar degradation products, phenols, phenoxycarboxylic acids, nitroaromatics, pharmaceuticals.

Octyl modified silica phase for SPE, not endcapped

Base material silica, pore size 60Å, particle size 45µm, specific surface 500m²/g, pH stability 2 to 8. Octyl phase, not endcapped, carbon content 8%, similar to C₁₈, however slightly more polar. Secondary interactions with polar compounds are more pronounced due to shorter alkyl chains.

Recommended applications: pesticides, PCBs.

Cyanopropyl modified silica phase for SPE

Base material silica, pore size 60Å, particle size 45µm, specific surface 500m²/g, pH stability 2-8, cyanopropyl phase, carbon content 5.5%, polar to mid-polar. In addition to weak hydrophobic interactions selective interactions are possible due to the high electron density of the CN group.

Recommended application: cyclosporins, carbohydrates.

Combination phase for SPE analysis of PAH

Special combination phase, cyanopropyl phase for selective adsorption of polycyclic aromatics via Π-Π interactions, unmodified silica phase for removal of polar compounds.

Recommended applications: Extraction of the 16 PAHs according to EPA from soil samples.