Protein blotting

Supported Nitrocellulose transfer membranes combine the binding characteristics of nitrocellulose membranes with the strength of nylon membranes. These membranes are used for DNA/RNA transfers requiring low background or for multiple hybridizations. For use with chemiluminescence and radiographic detection systems. BSA binding capacity up to 100 µg/cm².

The safety data sheets of these products are available under www.llg-labware.com.

Nitrocellulose membranes with a 0.2 µm pore size, offering excellent sensitivity, resolution and low background for Western blot protocols.

Features & Benefits:

• Low background and excellent signal-to-noise ratios, without the need for stringent washing conditions
• Compatible with a variety of Western blot labeling and detection systems
• Risk of ”blow-through“ reduced by high binding capacity of small proteins (< Mr 20 000)
• Optimized for chemiluminescent and fluorescent detection
• Long shelf life of bound proteins to the transfer membrane

Nitrocellulose membranes with a 0.45 µm pore size for use in Western blot protocols. Excellent sensitivity, resolution, and low background. for a wide range of laboratory applications.

Features & Benefits:

• Low background and excellent signal-to-noise ratios
• Compatible with a variety of labelling and detection systems
• High binding capacity
• Suitable for proteins with a wide range of molecular weights
• Ideal tool for your antibody purification and analysis workflow
• Long shelf life of bound proteins

PVDF blotting membrane with 0.45 µm pore size, hydrophobic and of high physical strength, for use with standard colorimetric and chemiluminescent detection methods for proteins of < Mr 20 000.

Features & Benefits:

• Protein binding capacity over 200 μg/cm² for easy signal detection.
• High protein retention even after harsh washing steps.
• High protein capture during transfer minimizes sample loss.
• Suitable for stripping and reprobing

Nitrocellulose blotting membrane with 0.45 µm pore size, for excellent sensitivity, resolution and low background.

Features & Benefits:

• High binding capacity (162 - 180 µg IgG/cm²)
• Suitable for proteins of a wide range of molecular weights
• 100% pure nitrocellulose membrane for use with all ECL Western blotting systems and protein blotting applications.
• Available in popular precut sizes for laboratory application

GB 003, GB 005. High purity. Very absorbent. Easy to use. Suitable for all protein and nucleic acid blotting techniques.

Low fluorescent, hydrophobic blotting membrane for protein transfer. Optimized for fluorescence detection in Western blotting applications.

• Protein binding capacity over 200 µg/cm²
• Higher sensitivity due to low background fluorescence
• Compatible with many blocking agents, transfer buffers and total protein blot stains
• Available in the standard precut sizes for laboratory applications