PCR

LABSOLUTE® offers a comprehensive range in the field of PCR consumables. These include tubes, strips of 8 tubes, 96-well plates and sealing films. In addition to the standard profile variants with 0.2 ml working volume, we also offer some low-profile versions with a working volume of 0.1 ml in the case of strips and plates.

• Suitable for all common thermal cyclers
• Optimal heat transfer and short cycle times through very thin and uniform wall thickness

Self-adhesive foils for safe sealing of 96-well PCR plates from LABSOLUTE® are free from human DNA and RNA, nucleases and pyrogens.

PCR adhesive foil: Polyester film with an acrylic-based adhesive, which is suitable for PCR, qPCR and other colorimetric assays, but has a low autofluorescence.

Cover and storage foil: PP foil with acrylic-based adhesive for universal closing of 96-well plates. Reliably protects the samples against evaporation and dust; removable, non-pierceable.

PCR and storage foil: The 20 µm thick aluminium foil with acrylic-based adhesive is easily pierceable without sticking the pipette tips or creating a vacuum.

qPCR adhesive foil: This qPCR foil is specially developed for qPCR applications. The pressure-sensitive adhesive is only released when the film comes into contact with the raised rims of the individual wells, thereby ensuring reliable sealing with high transparency and minimal autofluorescence.

Accessories PCR 96-well plates, PP

LABSOLUTE® offers a comprehensive range in the field of PCR consumables. These include tubes, strips of 8 tubes, 96-well plates and sealing films. In addition to the standard profile variants with 0.2 ml working volume, we also offer some low-profile versions with a working volume of 0.1 ml in the case of strips and plates.

• Suitable for all common thermal cyclers
• Optimal heat transfer and short cycle times through very thin and uniform wall thickness

The LABSOLUTE® cap strips are made of virgin PP and optimally seal strips of 8 tubes and 96-well plates. For easy orientation, the ends are marked differently. The strips with flat caps are suitable for fluorescence measurements (e.g. qPCR).

Accessories Strips of 4/8 PCR tubes, PP

LABSOLUTE® offers a comprehensive range in the field of PCR consumables. These include tubes, strips of 8 tubes, 96-well plates and sealing films. In addition to the standard profile variants with 0.2 ml working volume, we also offer some low-profile versions with a working volume of 0.1 ml in the case of strips and plates.

• Suitable for all common thermal cyclers
• Optimal heat transfer and short cycle times through very thin and uniform wall thickness

The LABSOLUTE® strips of 4/8 PCR tubes offer optimum heat transfer between the thermal cycler and the reaction mix through their extra thin and uniform vessel walls.

• Secure sealing reduces evaporation
• Available with flat and domed caps
• High quality PP
• Free from DNase, RNase and human DNA
• Standard profile with 0.2 ml working volume
• Low profile with 0.1 ml working volume
• Autoclavable at 121 °C

Accessories Racks for PCR tubes, 96 wells, PP Accessories PCR cap strips, PP Alternative PCR tubes 0.2 ml, PP Alternative PCR 96-well plates, PP

LABSOLUTE® offers a comprehensive range in the field of PCR consumables. These include tubes, strips of 8 tubes, 96-well plates and sealing films. In addition to the standard profile variants with 0.2 ml working volume, we also offer some low-profile versions with a working volume of 0.1 ml in the case of strips and plates.

• Suitable for all common thermal cyclers
• Optimal heat transfer and short cycle times through very thin and uniform wall thickness

The LABSOLUTE® 96-well PCR plates offer optimum heat transfer between the thermal cycler and the reaction mix through their extra thin and uniform vessel walls. The plates are sealable with adhesive films, heat sealing films and cover strips with flat and domed caps. The slightly raised rims of the individual wells reduce cross-contamination and evaporation of the samples. The black alphanumeric coding allows optimal sample distinction. All plates are free of DNase, RNase and human DNA.

96-well, skirted, low profile
Skirted 96-well PCR plates are rigid and suited for processing and transportation by automatic pipetting systems. Cut corner: H1.

96-well, semi-skirted
96-well semi-skirted PCR plates can be easily labelled or tagged with a barcode. Cut corner: A12.

96-well, for Roche® LightCycler® 480
The plates are perfect for use in LC480. Homogeneous white coloration with titanium dioxide and a smooth surface reduce interfering autofluorescence signals. Cut corner: H12.

96-well, non-skirted
96-well non-skirted PCR plates are suitable for a broad spectrum of thermal cyclers. Cut corner: H12.

Accessories PCR adhesive foils Accessories  Alternative PCR tubes 0.2 ml, PP Alternative PCR cap strips, PP

LABSOLUTE® offers a comprehensive range in the field of PCR consumables. These include tubes, strips of 8 tubes, 96-well plates and sealing films. In addition to the standard profile variants with 0.2 ml working volume, we also offer some low-profile versions with a working volume of 0.1 ml in the case of strips and plates.

• Suitable for all common thermal cyclers
• Optimal heat transfer and short cycle times through very thin and uniform wall thickness

The LABSOLUTE® PCR tubes offer optimum heat transfer between the thermal cycler and the reaction mix through their extra thin and uniform vessel walls.

• Compatible with all major thermal cyclers
• Available with flat and domed caps
• Easy and safe opening and closing with one hand
• High-quality PP (metal-free)
• Free from DNase, RNase, human DNA, pyrogens and PCR inhibitors
• Autoclavable at 121 °C

Accessories Racks for PCR tubes, 96 wells, PP Alternative Strips of 4/8 PCR tubes, PP Alternative PCR cap strips, PP Alternative PCR 96-well plates, PP

• Clear well bottom for sample visibility, with V-Bottom
• Compatible with thermocyclers of all leading brands
• Consistent wall thickness for uniform heat transfer
• Holes in the skirt for easy handling
• Virgin polypropylene, certified RNase-, DNase- and Endotoxin-free

For use in cyclers with screw-down or clip-down lids.

PCR Film: Clear film allows both the visual inspection of samples and fluorescence measurements.
PCR Foil: Aluminium, easy to pierce, no residue of adhesive on the pipette tips.
QPCR Foil: Adhesive seal optically clear, peelable, for QPCR and fluorescence applications.

• Individual tubes
• Compatible with all common thermocyclers
• 0.2 ml or 0.5 ml
• Individual attached flat or domed snap caps
• Consistent wall thickness for uniform heat transfer
• Cap design ensures fit and helps reduce sample evaporation
• Virgin polypropylene, certified RNase-, DNase- and Endotoxin-free

• Manufactured from high density polypropylene which is fully autoclavable. Excellent rack for "before" and "after" PCR sampling.
• This rack can be used alone or inserted in the workstation.
• This working/storage rack is designed to accommodate 96 pieces 0.2 ml PCR tubes or strips of 8 and 12 tubes.
• Tube wells are easly identified with alphanumeric,embossed numbers and letters.
• Translucent clear cover protects the tubes from dust and has a non-slip surface to allow safe storage of multiple racks.
• Dimensions (W x D x H): 125 x 88 x 31 mm

Removable hinged lid lies flat. Perfect for pre- or post-PCR sampling. Sturdy polypropylene rack accommodates individual 0.2ml tubes or strips of 8 or 12 tubes. Tube wells are easily identified with embossed numbers and letters. Autoclavable.

Polypropylene tubes for PCR experiments
Product characteristics of 0.2ml PCR tube:

• Contamination shield on attached lid
• Defined lid position (90°) due to special hinge
• Highly transparent
• Tight sealing
• Easy to open
• Can be used in all thermal cyclers with 0.2ml block format
• Certified being free from human DNA, DNase, RNase and PCR inhibitor

Product features of thin-walled 0.5ml PCR tube:

• frosted writing surface
• Autoclavable when open (at 121°C for 20 mins.)
• Highly transparent
• Easy to open
• Can be used in Mastercyclers with 0.5ml block format
• Certified being free from human DNA, DNase, RNase and PCR inhibitor

Accessories

In case the microplates, PCR- and Deepwellplates need not only to be covered, but also securely sealed, self-adhesive sealing films are available. These film sheets can be easily applied on the plates and removed also without the use of expensive equipment. They are available in different versions and are especially well-suited for storage or cell- and tissue-culture.

Purity, stability, color and other features to facilitate laboratory work.

• Extremely thin-walled polypropylene wells guarantee an optimal temperature transfer
• Exceptionally stable and torsion-resistant polycarbonate frame
• Elevated well edges ensure effective sealing and reduce the risk of cross-contamination
• Batch-tested and certified free of DNA, DNase, RNase and PCR-inhibitors (PCR clean), Eppendorf twin.tec^® microbiology additionally free of bacterial DNA
• Two versions available: With edge (skirted) or half-skirted (semi-skirted)
• Autoclavable up to 121 °C
• Available with bar code (on request)

The PCR-Cooler acts as a thermochromic temperature self-indicator: pink or light-blue (depending on the model chosen) when temperature exceeds +7°C, violet or dark blue when ice-cold.
The new Eppendorf PCR-Cooler reliably maintains a sample temperature of 0°C* for over an hour, changing colour when the temperature exceeds +7°C. Better than an ice bath, PCR-Cooler is simple to handle, space-saving and contamination-free, making it the perfect preparation for PCR reactions. The temperature of single tubes is indicated by the colour of the respective individual apertures. Furthermore, samples are prevented from freezing by the specially developed cone contour.
Eppendorf PCR-Cooler: the cooling unit in the universal 96-well format for 0.2ml and 0.5ml PCR tubes, for tube strips, plates and for individual tubes. The Eppendorf PCR-Cooler is a compact, innovative solution for protecting, transporting and storing sensitive samples and obviously also ideal for safely stopping reactions.

Application note:
By changing colour from violet to pink or dark blue to light blue, the PCR-Cooler shows when the temperature has exceeded +7°C. The crucial factor for sample cooling is the well colour in the PCR-Cooler.

Accessories PCR tubes, 0.2ml and 0.5ml, thin-walled Accessories Eppendorf twin.tec PCR plates 384 Accessories PCR plates, 96/384 well Eppendorf twin.tec®

BRAND has significantly expanded its product range of extra-thin-wall disposable products, which were specially developed to satisfy the demands of PCR applications.

Quality features

• Suitable for use in common thermocyclers
• PP, extra-thin uniform wall thicknesses to provide the optimal thermal transfer and short cycle times
• 96-well PCR plates with blue alphanumeric code and cut corner marking
• Highly clear flat covers optimally suited for qPCR
• DNase-, DNA- and RNase-free
• Autoclavable at 121°C (2 bar), acc. DIN EN 285
• qPCR: Products which are suitable for Real Time PCR (qPCR)

White PCR products offer significantly better results for qPCR, and are specifically recommended by many thermocycler manufacturers.
0.2 ml and 0.5 ml PCR tubes with attached caps are compatible with leading thermocyclers with heated lids. The caps are easy to open and close without tools, yet ensure a tight fit to reduce sample evaporation. The various colors of the PCR tubes allow fast sample classification.

Other colors available on request.

PCR-Cooler made of PP with lid made of PC. Ice-free cooling system for PCR preparation and storage of 0.2 and 0.5 ml PCR tubes and strips as well as 96 well plates in standard and low profile well shape. Maintains sample temperature of less than 5 °C for up to 3 hours (with lid off). Maintains the entire 96-well plate at a temperature of 0 °C for 1 hour. When the temperature ceiling of 6 °C has been exceeded, users are signaled by a contrasting color change of the cooler, or a single well.

• 2 colour options: Purple to pink or dark blue to light blue
• Ice-free cooling reduces the risk of contamination
• Fully sealed and sonic welded cooler keeps coolant contained
• Detachable lid serves also as a stand
• Lid is suitable for left and right handed users
• Ergonomic textured finger grips provide a secure hold, especially when using gloves
• Can be used for freezer storage down to -80 °C
• Alphanumeric identification
• 2 years warranty

The rack can be fitted into the box. Together, they create an ideal workstation for 0.2 ml PCR tubes, available in individual, 8-tube strip and 12 tube strip or 96-well plate formats. The box with transparent lid can be stacked to save space and is temperature resistant from -80 to +121°C. Assorted colours (red, yellow, purple, green, blue).

BRAND has significantly expanded its product range of extra-thin-wall disposable products, which were specially developed to satisfy the demands of PCR applications.

Quality features

• Suitable for use in common thermocyclers
• PP, extra-thin uniform wall thicknesses to provide the optimal thermal transfer and short cycle times
• 96-well PCR plates with blue alphanumeric code and cut corner marking
• Highly clear flat covers optimally suited for qPCR
• DNase-, DNA- and RNase-free
• Autoclavable at 121°C (2 bar), acc. DIN EN 285
• qPCR: Products which are suitable for Real Time PCR (qPCR)

White PCR products offer significantly better results for qPCR, and are specifically recommended by many thermocycler manufacturers.
Strips of 8 or 12 connected PCR tubes (0.2 ml). Cap strips consisting of 8 or 12 domed caps (available separately) provide tight seal during all PCR cycles, thus protecting samples from cross-contamination. The tubes have a holding strap at one end, and the cover strips on each lid have a small lip on the side for careful, contamination-free opening. Consistent tube wall thickness allows rapid heat transfer along the entire exterior tube surface. Made of pure, virgin polypropylene, free from RNase-, DNase- and DNA. Autoclavable at 121 °C (2 bar), according to DIN EN 285.

Other colors on request.

Accessories PCR cap tool, PA

Ideal for sealing PCR plates.

• PCR clean: Certified free from human DNA, DNase, RNase and PCR inhibitor
• PCR Film: Allows visual monitoring of samples
• PCR Foil: Easy to pierce, no sticking of the pipette tips (ideal for automated systems)
• Heat Sealing: Position the film or the foil with the adhesive edges on the plate. Seal the plate using the heat sealer
• Sealing mats are reusable and autoclavable

Accessories

BRAND has significantly expanded its product range of extra-thin-wall disposable products, which were specially developed to satisfy the demands of PCR applications.

Quality features

• Suitable for use in common thermocyclers
• PP, extra-thin uniform wall thicknesses to provide the optimal thermal transfer and short cycle times
• 96-well PCR plates with blue alphanumeric code and cut corner marking
• Highly clear flat covers optimally suited for qPCR
• DNase-, DNA- and RNase-free
• Autoclavable at 121°C (2 bar), acc. DIN EN 285
• qPCR: Products which are suitable for Real Time PCR (qPCR)

White PCR products offer significantly better results for qPCR, and are specifically recommended by many thermocycler manufacturers.
Eight 0.2 ml PCR tubes joined together in a strip with attached, hinged 8-cap strips. PP. The hinged, domed cap makes opening and closing the tubes with one hand easier.

Bench-top models for protection against contamination during a variety of DNA/RNA procedures.

• UV surface irradiation, via 1 x 25 W 254 nm open UV lamp or 2 x 30 W open UV lamp (UVT-S-AR)
• Patented high intensity UV air cleaner, 25 m³/hour cleaner-recirculator continuous air flow with 1cm UV irradiation distance
• UVlamp in the upper hood disinfects the working area, inactivating DNA/RNA fragments during 15-30 min of exposure
• UV exposure control, 24 hour digital timer
• Convenient, easy to use digital timer for accurate control of UV exposure
• White lamp provides local illumination of the workplace to optimise visual control during operations
• Shock proof glass panels, metal framework and stainless steel work surface
• Automatic switch-off of open UV light when door opened
• UVT-B-AR is available with 1 x access port or 1 x internal power socket, UVT-S-AR with 3 x internal power socket, UVC/T-M-AR with 1 x access port

Specifications
External dimensions (W x D x H):	690 x 585 x 555 mm
Working area dimensions (W x D):	650 x 475 mm
Weight:	32 kg

Accessories

The use of real-time PCR (qPCR) is becoming increasingly widespread. The line of white PCR products by BRAND offers significantly better results in this application than transparent tubes or plates. The different products in this line are uniformly colored with TiO2 (titanium dioxide), so that in combination with the smooth surfaces, the optimum reflection of the fluorescence signal is provided.
The ultra thin-walled PCR plate design facilitates constant, rapid and precise heat transfer. The smooth vessel interior minimizes the binding of enzymes and nucleic acid to the walls. The rim of the wells are designed to protect against cross-contamination, allowing a reliable seal with the sealing mats tailored to the plates. With alphanumeric code and cut corner for easy orientation.

96-well, non-skirted
PP. 0.2 ml wells. 96-well non-skirted PCR plates are suitable for most commercially available thermocyclers.

96-well, semi-skirted
PP. 0.2 ml wells. 96-well semi-skirted PCR plates can easily be labelled or tagged with a barcode.

96-well, semi-skirted. Suitable for Roche^® LightCycler^® 480 and other thermocyclers
PP. 0.15 ml wells. Semi-skirted. Available with or without self-adhesive sealing film (polyester, high-transparent, for qPCR).

96-well, skirted
PP. 0.2 ml wells. 96-well skirted PCR plates are especially rigid, and are optimally suited for use with automatic pipetting systems and for being transported.

Alternative 384 well PCR-Plates, white, for real-time PCR

Removable hinged lid fits both sides. Flip this polypropylene reversible rack to the size of tubes you need. PCR side of the rack has 168 wells that hold 0.2ml tubes. The wells accommodate 8- or 12-tube strips or individual tubes. The opposite side of the rack has 40 wells that hold 0.5ml tubes. Both sides of the rack have 12 wells for 1.5ml tubes. Dimensions (L x W x H): 206 x 131 x 54mm. Autoclavable.

Ultra-thin wall design facilitates rapid and steady heat transfer. Smooth interior tube surface minimises binding of enzymes and nucleic acids. Raised rims of wells protect against cross-contamination and provide tight seal particularly in combination with BRANDás new sealing mats.
0.2 ml wells. The 24 / 48 well plates reduce material costs and allow work on a compact PCR plate even with small sample throughputs. Suitable for most types of standard thermal cyclers.

Accessories PCR Mini cooler, PP Alternative PCR Plates 384-well, PP

The use of real-time PCR (qPCR) is becoming increasingly widespread. The line of white PCR products by BRAND offers significantly better results in this application than transparent tubes or plates. The different products in this line are uniformly colored with TiO2 (titanium dioxide), so that in combination with the smooth surfaces, the optimum reflection of the fluorescence signal is provided.
White, PP. Suitable for qPCR. 0.03ml tubes.

Alternative 96 well PCR-Plates, PP, for PCR or qPCR

• Accepts a wide range of microplates and heat seals
• User-friendly control system
• Large digital display shows all operating parameters
• Adjustable sealing temperature: 100°C to 190°C, or no heat
• Precise temperature, timing and pressure for consistent sealing

Specifications
Dimensions (W x D x H):	171mm x 324mm x 362mm
Weight:	12kg
Sealing Temperature:	Off, 100 to 190°C (increments of 1.0°C)
Temperature Accuracy:	±1.0°C
Temperature Uniformity:	±1.0°C
Sealing Time:	0.5 to 10 sec (incements of 0.1 sec)
Compatible Plate Materials:	PP (Polypropylene), PS (Polystyrene), PE (Polyethylene)
Compatible Plate Types:	Standard Assay Plates, Deep-Well Storage plates, PCR Plates (Non-Skirted, Semi-Skirted, Full-Skirted)
Maximum Plate Height:	45mm
Compatible Sealing Film Types:	Foil Polypropylene laminate, Clear Polyester-polypropylene laminate, Clear polymer, Thin clear polymer, Foil-laminate, Foil

White. The sealing paddle helps to attach the self-adhesive film to the plate with a uniform pressure. Especially suited for plates with raised rim.

The Matrix™ SepraSeal sealing mats are suitable for securely sealing sample tubes with or without 2D code. For sample access, the caps can be removed with the practical cap removal tool (Cat.-No. 6.254 390, please order seperately) or pierced once. The pre-slit versions are suitable for multiple piercing. The self-sealing function of the pre-slitted elastomer maintains sample integrity.

• For storage down to -20 °C
• Individual closure or closure of 96 sample tubes simultaneously possible
• Good chemical resistance
• Low evaporation rate

With the Matrix™ DuraSeal sealing mat (Cat.-No. 4.658 312), sample tubes are permanently sealed. The sealing mat is pre-slit for sample access and has a v-shaped design. The special elastomer ensures that the integrity of the sample is maintained. Suitable for sample storage down to -80 °C.

For PP, PS and COC microplates. Transparent, Peelable.

Application:	Quantitative PCR, fluorescence / colorimetric optical assays.
Sealing:	2 to 3 sec 170 to 175°C
Seal Integrity:	-80°C to +110°C, PCR with pressure lids

Clear Polyester backed film, with non peelable seal on Polypropylene microplates.

Application:	Quantitative PCR, optical assays, Roche Lightcycler480, waterbath cycling.
Sealing:	2 to 3 sec 170 to 175°C.
Seal Integrity:	-80°C to 110°C

High-performance plates for manual or automated applications. Suitable for sample storage and preparation, bacteria and yeast cultivation, DNA and RNA isolation, protein analysis and plasmid purification.

• Clear wells
• Frame, white or coloured
• High-contrast, alphanumeric lettering
• Autoclavable (121 °C, 20 min)
• Centrifugation resistance up to 6000 × g
• Rounded RecoverMax^®-Well-Design
• Elevated well frames for reliable sealing, even with heat sealing
• Stackable
• PCR clean: free of human DNA, DNase, RNase and PCR inhibitors or sterile

Alternative

Polyester/Aluminium laminate. Peelable. PP and COC microplates.

Application:	Compound storage. 100% DMSO resistance at room temp. Good on 1536well plates
Sealing:	2 to 3 sec 170 to 175°C
Seal Integrity:	-80°C to +80°C

For PP and PS microplates. Piercable and Peelable. Good solvent / DMSO resistance.

Application:	Compound storage, PCR
Automated workflows:	Piercing and oversealing possible
Sealing:	2 to 3 sec 170 to 175°C
Seal Integrity:	-80°C to +120°C

Cell culture is increasing in popularity in the research and development area. Outside of basic research, cells are cultivated today for a number of reasons, including the production of proteins and in particular as assay systems. As cell cultures can sometimes be quite demanding regarding their environment, the disposables used for cultivation have to be of highest quality. The different cell culture surfaces allow the optimum combination between microplate and specific cell line.

Standard plate for the cultivation of adherent cell cultures. PS-surface with different chemical groups, like e.g. carboxyl and hydroxyl groups, that are freely accessible. Surface is hydrophilic compared with non-treated PS. Incl. one lid per package.

• Sterile
• Manufactured under ISO class 7 cleanroom conditions
• Orange or embossed alphanumeric coding
• Free from endotoxins (< 0.01 EU/ml), DNase, DNA, RNase, non-cytotoxic (according to ISO 10993)
• ANSI/SLAS format

Alternative Microplates for Cell Culture BRANDplates® cellGrade™ plus Alternative Microplates for Cell Culture BRANDplates® cellGrade™ premium Alternative Microplates for Cell Culture BRANDplates® inertGrade™

The thin wall construction optimises the heat transfer from the block to the reaction solution.

Properties:

• Thin wall for optimal heat transfer
• Free of detectable DNase, RNase, human DNA
• Non-Pyrogenic
• For Research Use Only, not for use in diagnostic procedures, unless otherwise indicated

With transparent lid.
For protecting samples prior to warming. The mini PCR cooler keeps samples at 4 °C for approx. 3 hours. The insulating gel changes from violet to pink at 7°C. Suitable for 0.2 ml single vessels, 8-strips, 12-strips as well as 96-well PCR plates.

Ultra-thin wall design facilitates rapid and steady heat transfer. Smooth interior tube surface minimises binding of enzymes and nucleic acids. Raised rims of wells protect against cross-contamination and provide tight seal particularly in combination with BRANDás new sealing mats.
BIO-CERT^® PCR-Quality. The thin walls allow fast transmission of the temperature specified by the cycler, thus reducing cycle times. The stable design of the plates makes them ideal for automation, as they can be gripped by robot systems without twisting. To minimize evaporation with small sample volumes up to 2 µl, the plates can be sealed with appropriate sealing films.

• For automatic high-throughput analyses
• DNase-, RNase-, DNA-, and endotoxin free
• Manufactured under controlled cleanroom conditions

Accessories PCR Mini cooler, PP Alternative 24 / 48 well PCR Plates, non-skirted, PP, for qPCR

BRAND has significantly expanded its product range of extra-thin-wall disposable products, which were specially developed to satisfy the demands of PCR applications.

Quality features

• Suitable for use in common thermocyclers
• PP, extra-thin uniform wall thicknesses to provide the optimal thermal transfer and short cycle times
• 96-well PCR plates with blue alphanumeric code and cut corner marking
• Highly clear flat covers optimally suited for qPCR
• DNase-, DNA- and RNase-free
• Autoclavable at 121°C (2 bar), acc. DIN EN 285
• qPCR: Products which are suitable for Real Time PCR (qPCR)

White PCR products offer significantly better results for qPCR, and are specifically recommended by many thermocycler manufacturers.
The strips of 8 PCR tubes with attached, flat individual caps provide an ideal protection against contamination. The highly transparent cap makes them ideal for a quantitative Real Time PCR. Available in Standard (0.2 ml) or Low Profile (0.15 ml).

Alternative Strips of 8 PCR tubes with attached cap strips

Product features of twin.tec PCR Plates 384:

• U-shaped inner conture of the wells for excellent protection control
• exceptional rigidity and flexure resistance
• continuous skirt for compatibility with automated systems
• semi-skirt for labelling with automated systems
• stackable
• 8 holes in the skirt aid plate positioning and removal from the thermal cycler block e.g. with grippers.
• within SBS footprint recommendations (127.76 ± 0.25 mm x 85.47 ± 0.25 mm)
• suitable for all 384-Well-thermocycler
• 45 µl max. well volume

Designed to fit in a standard 53mm freezer rack, this 144-place PCR Tube Freezer Box will hold individual and/or strip 0.2ml PCR tubes.

• Designed for ULT freezer storage at temperatures down to -86°C
• Durable and impervious to moisture
• Clear polycarbonate lid and base and alpha-numeric index make it easy to identify samples within the box
• Two boxes may be stacked to fit together in one space in a 53mm freezer storage rack if using flat-capped PCR tubes

For PP and PS microplates. Excellently piercable. Not peelable. Very good solvent / DMSO resistance.

Application:	Compound storage, PCR
Automated workflows:	Piercing and oversealing possible
Sealing:	2 to 3 sec 170 to 175°C
Seal Integrity:	-80°C to +120°C.

The new Prime Pro 48 real time PCR system from Techne (UK) is a high specification, economically priced real-time thermocycler. The Prime Pro 48 system has a unique high thermal block uniformity (± 0.1°C). This uniformity as well as validated sample volumes of down to 5µl enable an optimized reaction run time for 40 cycles down to only 15 minutes. The uniform temperature across the plate is achieved with a unique heating and cooling system that quickly enables a uniform temperature of ±0.1°C in every well of the block within a fraction of a second of reaching each well temperature. This high uniformity eliminates the need for triplicates which reduces reagent running costs. Prime Pro 48 accommodates a unique and economical 48-well PCR plate, the size of which is only 1/8 the size of a standard 96-well plate. The well format mirrors a 384-well plate therefore allowing the use of a 16-channel micropipette. The small plate cuts reagent costs in half whilst still producing a strong fluorescent signal. Applications include e.g. absolute and relative quantification, Allelic Discrimination and High Resolution Melt.

Features:

• Validated volume per well: 5 to 20 µl
• High temperature uniformity of ±0.1°C across the whole block instantly after every temperature change
• 40 cycles in 40 minutes before optimization, and down to only 15 minutes when optimized
• Genotype in 4 minutes with over 99% accuracy
• Patented "Adaptive LED Control" minimizes optical artifacts and prevents premature detector saturation
• Dual LED excitation (452nm to 486nm and 542nm to 582nm)
• 4 emission filters (505nm to 545nm, 562nm to 596nm, 604nm to 644nm, 665nm to 705nm)
• Data is always collected in all four filters for all wells
• Distinguishes 5000 and 10000 template copies with 99 percent confidence

Additional features:

• 48-well block
• MIQE compliant
• 400 analyte specific qPCR reagent kits available
• No calibration required
• Calibrated dyes: SYBR^®, FAMTM, HEXTM, ROXTM, Cy^®5.
• Additional dyes within the filter wavelength range are supported with no additional calibration required
• Unlimited software license
• 1 year warranty

Custom 48-well qPCR plates and corresponding seals have to be ordered separately.

Specifications
Temperature range:	30 to 100°C
Temperature stability:	±0.1°C
Temperature uniformity:	±0.1°C within 1sec
Average ramp rate:	5.5°C/sec
Dimensions (W x D x H):	345 x 310 x 320mm
Weight:	13.6kg

The Alpha Cycler family form PCRmax provides a range of features which are specifically designed to meet the needs of modern researchers when amplifying DNA. Given the flexibility in format and block options (96 or 384 well) scaleability is core in the Alpha Cycler range. Alpha Cycler software has features such as recently used programmes, allowing users to quickly access their most commonly used protocols without the need to navigate through folders to find it, individual user logins, with protected protocols. The Alpha Cycler systems also contain a novel programme wizard which allows users to define a protocol based specifically on their primer sequence and template source. Primer sequences or T_M"s are entered, amplicon length and source defined and the on board algorithm generates an optimised protocol for your assay. The Alpha Cyclers range of block options allow for use of the most commonly used consumables from different manufacturers and includes 96 well plates (0.2 ml), tubes (0.2 ml), strip tubes (0.2 ml) and 384 well plates.

• Single, dual or four block options available with either 96 or 384 well gradient blocks
• Multi-block units have totally independent blocks for maximum flexibility
• HD Android driven touchscreen interface for simplified programming
• Generate and send instrument reports to diagnose the health of your system and identify any issues before they impact users work
• Lock programs with user defined password
• Alpha Track mobile app allows remote monitoring of run progress

Alpha Track App (free of charge): Track the progress of runs and health of your system remotely via the mobile app
The Alpha Track Application is used in conjunction with your Alpha Cycler and allows users to:

• Remotely track the progress of runs
• Send a health report for diagnosis by the technical support team
• Be notified of any issues and prevent system failures before occurring

Specifications
Block temperature:	10 to 100 °C
Block uniformity at 55 °C:	±0.3 °C
Temperature accuracy at 55 °C:	±0.25 °C
Max. Heating rate:	3.4 °C/s

This simple to program PCR thermocycler unit is compact in design, programming is intuitive with the large display and multiple pre-programmed templates supplied with the unit. The OptiMax comes with standard built in 2-step, temperature optimization, touchdown and time increment protocols that are easy to adjust to meet cycling needs. Select lid temperature or turn it off.

• Flexible Programming with FlexTemp™ technology
• Fast ramp rates 5 °C heating / 3.5 °C cooling
• No condensation after overnight cooling at 4 °C
• Individual temperature setting for each block
• Password protected programs
• Simple user interface
• With USB and RS232 interface

Specifications:
Sample capacity:	1 x 96 well plate, 12 x strip tubes (8 x 0.2 ml), 96 x 0.2 ml tubes
Programmable temperature range:	4 °C ... 99.9 °C
Temperature control:	Calculated or block
Temperature accuracy/uniformity:	±0.5 °C/±0.5 °C
Heating/cooling method:	Peltier
Max. heating/cooling rate:	±5 °C/±3.5 °C per second
Temperature range of 6-segment blocks:	30 °C ... 99 °C (can be set independently)
Maximum temperature difference between 6-segment blocks:	±24 °C
Programmable lid temperature:	60 °C ... 65 °C, 100 °C ... 115 °C
Program memory: User program folders:	200 complete programs 50 sets
Dimensions (W x D x H):	240 x 420 x 250 mm
Weight:	9 kg
Supply requirements:	230 V, 50/60 Hz*

Mid-size thermocyclers - find space on any laboratory bench

• Approved cycler with new design
• With and without gradient function
• Gradient upgrade from ³PRIMEX to ³PRIMEG
• 3.5" colour touch screen with graphical display
• Height-adjustable and heatable lid, temperature from 100°C to 115°C, with ON/OFF switch and automatic switch off against overheating
• Password protected
• Auto-Restart
• USB port
• 4 years warranty for the Prime Thermocycler

Specifications
Block temperature:	+4°C to +100°C
Temperature gradient (only 3PRIMEG or 3PRIMEX with upgrade):	+30°C to +80°C
Max. Gradient:	+14°C
Block uniformity at 55°C:	<±0.3°C
Max. Heating rate:	3.0°C/s
Dimensions (W x D x H):	210 x 350 x 180

Alternative Thermocycler Alpha Cycler

Made of highly transparent PP without additives. Ultra-thin PP ensures optimum heat transfer during amplification. Comprehensive thermal cycler compatibility. Reaction vessels with flat lids for Real Time PCR / quantitative PCR have matted lids for easier labeling.

Features & Benefits:

• Packaging labeling with production symbols for easier identification and storage
• Free of detectable DNase, RNase and human DNA
• Free of detectable endotoxins

For cell culture applications, sample preparation and storage of samples and solutions.

• Conical
• Temperature resistant from -86 °C to 100 °C

Accessories Eppendorf Tube Racks, PP Accessories Accessories for Eppendorf Tubes® 25 ml, PP Alternative  Alternative Eppendorf Tubes®, PP, amber, with screw cap, HDPE Alternative  Alternative Eppendorf Tubes® 25 ml, PP, starter pack

These UV sterilisation cabinets provide a convenient area for setting up PCR reactions in a nucleic acid free environment thus limiting PCR reaction contamination. Acting effectively as a low cost alternative to a Cleanroom, the powerful UV lights on the cabinets denature nucleic acids in 5 to 30 minutes making them unsuitable for amplification. The cabinet incorporates safety features to prevent user exposure to UV light. The UV lights themselves are timer controlled and there are safety switches on the cabinets doors which turn off the UV lights when opened. Constructed from 10 mm acrylic, the cabinets also act as efficient shields from beta radiation emissions and can therefore be safely used with isotopes such as ³²P.

Specifications	Maxi / Midi / Mini
UV Lights:	4 x 15 W
White Lights:	15
Dimensions (H x W x D):	770 x 580 x 420 mm / 620 x 580 x 420 mm / 450 x 580 x 350 mm
Weight:	19 kg / 14.6 kg / 12 kg

Two in one mini kit for gel extraction and PCR clean up.

Sample material:	Agarose gel, PCR reaction mixture
Sample amount:	< 400 µL PCR reaction mixture, < 400 µg agarose gel
Fragment size:	50 bp−?approx. 20 kbp
Typical recovery:	70−?95 %
Elution volume:	15−?30 µl
Downstream application:	Cloning, PCR, Restriction analysis, Sequencing

Adhesive sealers are an interesting alternative to heat sealing systems or CapMats. They are easy to use and there is a lower risk of cross-contamination because they are used as disposable products.

Properties:

• Free of detectable DNase, RNase, human DNA
• Non-Pyrogenic
• Highly transparent film for optical measurements
• Ideally suited for protein crystallography
• Coated with a silicone adhesive
• Only sticks when the film surface is pressed on
• The film does not adhere to gloves
• Substances in powder form and biological model organisms like Drosophila melanogaster or Caenorhabditis elegans do not stick to vessels sealed with VIEWseal™
• Temperature resistant from - 80°C to + 110°C
• Suitable for PCR applications and sample storage
• Minimal autofluorescence

BRAND has significantly expanded its product range of extra-thin-wall disposable products, which were specially developed to satisfy the demands of PCR applications.

Quality features

• Suitable for use in common thermocyclers
• PP, extra-thin uniform wall thicknesses to provide the optimal thermal transfer and short cycle times
• 96-well PCR plates with blue alphanumeric code and cut corner marking
• Highly clear flat covers optimally suited for qPCR
• DNase-, DNA- and RNase-free
• Autoclavable at 121°C (2 bar), acc. DIN EN 285
• qPCR: Products which are suitable for Real Time PCR (qPCR)

White PCR products offer significantly better results for qPCR, and are specifically recommended by many thermocycler manufacturers.
For PCR-tubes with detached cap strips.

Accessories PCR cap tool, PA

The use of the 96 well format allows the scale up of basic PCR work, while the 384 well format is ideal for high-throughput screening projects.

Properties:

• Ultra thin polypropylene for optimal heat transfer from the thermoblock to the reaction solution
• Free of detectable DNase, RNase, human DNA
• Non-Pyrogenic
• Sealable with sealers SILVERseal™ , VIEWseal™ and AMPLIseal™ or with compatible 8-cap strips
• For Research Use Only, not for use in diagnostic procedures, unless otherwise indicated

DNA LoBind plates for maximum sample recovery from nucleic acids by reducing the binding of the sample to the surface. Suitable for preparation or storage of DNA and RNA samples, forensic trace analysis, preparation of dilution series in quantitative qPCR, sample preparation in next-generation sequencing and creation of genome or oligonucleotide libraries.

• PCR clean: free of human DNA, DNase, RNase and PCR inhibitors
• High-contrast, alphanumeric lettering
• Frame white or blue
• Rounded RecoverMax^®-Well-Design
• Elevated well frames for reliable sealing

Alternative

Stable, flexible protection of samples during short-term storage.

The Fast PCR tube strips made of polyethylene transfer heat much better and faster than tubes made of traditional plastics. They are particularly suitable for use with Fast PCR reagent kits and for cyclers with high heating and cooling rates. Under these conditions, the following results higher yield and significant time savings.

• Suitable for PCR, Fast PCR and Real Time PCR
• Increased thermal conductivity through PE tubes
• Higher yield and significant time savings
• Can be used in any cycler for 100 µL volumes
• Lid strip PP
• With flat or domed lid
• Certified PCR clean

Alternative

The Mastercycler X50 family offers tempering rates of up to 10 °C/s and is the ideal cycler family for routine applications as well as state-of-the-art molecular biological research. The Mastercycler X50 family consists of units with user-friendly VisioNizeR touchscreen control panel or eco units, which are externally controlled. A low noise level, low energy consumption and the versatile flexlidR concept round off the functions of this powerful and convenient PCR cycler.

Features & benefits:

• Networkable with nine other eco devices
• With CycleManager X50 as central control unit, up to 50 eco devices can be connected to one computer or laptop and each one can be controlled and monitored individually and independently
• The intuitive interface manages who has access to the machine and allows you to set user access levels

For measuring absorbance assays in the visible and UV range, fluorescence and chemiluminescence assays, DNA- and protein concentration with absorbance or fluorescent dyes, cell based assays, cell viability and apoptosis assays. UV-VIS Microplates feature an ultrathin film bottom for good light transmission in the UV range. Black Eppendorf Microplates offer an good signal-to-noise ratio. White Eppendorf Microplates are optimized for highest sensitivity in the detection of luminescence signals by maximizing reflection.

Alternative

Suitable for sample storage and preparation, protein analysis and genotyping.

• High-contrast, alphanumeric lettering
• Wells transparent with white frame
• Rounded RecoverMax^®-Well-Design
• Elevated well frames for reliable sealing, even with heat sealing
• Temperaturresistance from -86 °C to 100 °C
• Autoclavable (121 °C, 20 min)
• Centrifugation resistance up to 6000 × g
• PCR clean: free of human DNA, DNase, RNase and PCR inhibitors or sterile

Alternative

DNA LoBind plates for maximum sample recovery from nucleic acids by reducing the binding of the sample to the surface. Suitable for preparation or storage of DNA and RNA samples, forensic trace analysis, preparation of dilution series in quantitative qPCR, sample preparation in next-generation sequencing and creation of genome or oligonucleotide libraries.

• V-bottom wells
• PCR clean: free of human DNA, DNase, RNase and PCR inhibitors
• High-contrast, alphanumeric lettering
• Wells transparent with white frame
• Rounded RecoverMax^®-Well-Design
• Elevated well frames for reliable sealing

Alternative

Protein LoBind plates are specially designed for proteomics and other areas of protein research. Suitable for preparation or storage of protein, peptide or antibody samples, enzymatic assays, storage of virus stock solutions or cell suspensions and sample processing in toxicology.

• V-bottom wells
• High-contrast, alphanumeric lettering
• Wells transparent with white frame
• Rounded RecoverMax^®-Well-Design
• Elevated well frames for reliable sealing
• PCR clean: free of human DNA, DNase, RNase and PCR inhibitors

Alternative

Fully compatible to all current standard-equipments (reading and washing devices). Safe stacking, no danger of slipping. Appropriate gripping surfaces at the side. Frosted surface of the plates prevents errors from extraneous light. Alpha-numerical well identification for easy sample location. Space for identification marking on the side surface. Covers with condensation-rings to avoid medium-losses and uneven evaporation. Lid orientation corners reduce risk of cross-contaminations.

U-Well: No edges, therefore simple and clean to pipette into, for +/- evaluations, especially suitable for agglutination-tests.
V-Well: Especially good for releasing the "last drop" from the pipette, for +/- evaluations, suitable for storage of samples.
F-Well: For precise optical measurements, for microscopic applications (Bottom Reading), rims at the bases prevent a scuffing of the optical surface, widened cell-growth-surface.

Rapid methods for the detection of mycoplasma contamination are essential for improving monitoring of the production process of biologicals. With the use of the Microsart^® Mycoplasma the testing time can be reduced from weeks to just 3 hours.

PS. Radiation-sterilized. With cover. Ideal for screening DNA libraries. Holder for membranes for dot-blotting. Vessels for denaturing, hybridization and washing stages. Useful for storing membranes. Colonies easy to recognize as material is transparent. Can be stacked safely. High and robust walls. Can be frozen down to -20 °C.
Can also be supplied with cell culture surface.

BRAND^® Premium PCR plates

• ultra-thin walled
• even and rapid heating of all samples
• reinforced plate deck
• no warping, no wiggling, for use with robots
• free of DNase, DNA, RNase and endotoxins
• suitable for qPCR

BRAND^® PCR Sealing film

• highly transparent for qPCR
• non-tacky surface
• press to seal
• convenient end tabs
• no annoying adhesive residues

Every package includes 50 PCR plates 96-well and 50 qPCR sealing films (Art. Nr. 4.000 237).

The PCR^® rack base is made from robust ABS construction, and the lid is made from PC. Versatile Rack for use on a bench top or for freezer storage.

• Storing of tubes down to -80 °C
• Holds 0.2 ml individual tubes or strips
• Ambidextrous
• Detachable lid
• Stackable

Cap strips

• strips with 8 microcaps
• easy and effective sealing of PCR plates and PCR Tube Strips
• easy to remove using extension at end of strip
• autoclavable (at 121°C for 20 min.)
• certified free of human DNA, DNase, RNase and PCR Inhibitor

Effectively seals all microplate formats for storage, PCR, microscopy, culture and protection. Pre-cut, plate-sized sheets.

Type 1) General use, PCR, storage, photo-sensitive samples
Type 2) Cell Culture, sterile applications
Type 3) Microscopy, good optics, low auto-fluorescence, DMSO compatible
Type 4) Breathable, cell culture

Transparent strip tubes plus frosted cap strips with extensions for easy handling and sample identification due to easy write-on.

• Compatible with Rotor-Gene Q Real-Time PCR Cycler
• Leakproof
• Smooth, uniform and thin walls for good and reliable results
• Low background fluorescence
• Optimum signal to noise ratio
• DNase, RNase and Human DNA-free