DNA and RNA isolation
Highly purified DNA and RNA samples can be extracted from a wide variety of different sources and are extremely important for numerous research and clinical applications. Isolation of nucleic acids often involves lysis of cell membranes and/or sample homogenisation. A variety of high quality purification kits, reagents and accessories from Macherey-Nagel, IST Innuscreen, Promega, NEB and other suppliers are available from Th. Geyer.
Plasmid Purification-Kits I-Blue Mini / Midi
For rapid and efficient extraction of plasmid DNA from bacterial cells.
The method is based on a combination of alkaline lysis and RNase treatment to obtain a clear lysate with minimal genomic DNA and RNA contamination. The bound plasmid DNA is washed and finally eluted by adding a buffer. The test kit contains the I-Blue Lysis Buffer, an optional colour indicator. The use of this indicator prevents common handling errors that impair efficient cell lysis and neutralization....
