Cultivation

The cell culture flasks from LABSOLUTE® are made exclusively of PS, corresponding to the highest international standards (USP Class VI). A short, wide and angled neck allows an easy handling while the risk of contamination is minimized. The treatment of surfaces allows adherent cells a uniform and even cell adhesion.

• Short and wide sloping neck for easy access
• Easily stackable
• Both flasks sides have engraved graduation with a labelling area
• Screw cap with a hydrophobic 0.22 μm filter for ventilation
• Packed upright in resealable bags
• Surface is treated with vacuum gas plasma
• Sterilized by radiation (SAL 10^-6)

Accessories Cell scrapers and cell lifters, sterile Alternative Cell culture dishes, treated, sterile, PS Alternative Cell culture multiwell plates, treated, sterile, PS

The LABSOLUTE® filtration units are ideal for filtration of buffer solutions, cell culture media, microbiological media and other biological fluids. The PES membrane guarantees good flow through times and low protein binding.

• High traceability thanks to expiry date and Lot. no. on each filter
• 0.2 μm: Ideal for sterilization applications
• 0.45 μm: Ideal for clarifying buffer solutions and nutrient media preparation
• Filtrate bottle with lid for convenient storage of the filtered solution
• Individually packaged
• Different volumes available
• Pyrogen free and not cytotoxic
• Quick connection for hoses with an inside diameter of 8 to 12 mm
• Gamma sterilized (SAL 10^-6)

The complete filtration unit consists of a filter funnel with vacuum connection and lid, the filtrate bottle and the associated screw cap.

Accessories Filter funnel, sterile, PES Accessories Filtrate bottle, sterile, PS

The LABSOLUTE® cell strainers are made of a strong nylon mesh and are characterized by an even distribution of the mesh pores. With the sterile cell sieves, cell suspensions can be quickly and easily filtered to collect primary cells or prepare samples for flow cytometry.

• Available in three mesh sizes: 40, 70 and 100 μm
• Color coded for easy distinction
• Extended edge allows easy handling even with tweezers
• Designed to fit standard 50 ml centrifuge tubes
• DNase, RNase and pyrogen-free
• Individually packed, gamma-irradiated (SAL 10^-6)

Accessories Centrifuge tubes, PP

The LABSOLUTE® cell scrapers and lifters enable easy and efficient cell harvest. Optimize your cell harvest by using the cell scraper for cell culture flasks and cell lifter for dishes. The handle is made of ABS and allows the controlled use of the thin, flexible blade made of TPE.

• Scraper in two different handle lengths (25 cm with 2 cm blade and 39 cm with 3 cm blade)
• Two blade positions: scraper and lifter
• Individually wrapped in paper/plastic blister packs

Accessories Cell culture dishes, treated, sterile, PS Accessories Cell culture flasks, treated, sterile, PS Accessories Cell culture multiwell plates, treated, sterile, PS

The LABSOLUTE® multiwell tissue culture plates are suitable for handling multiple samples in a single experiment. From 4-well up to 96-well plates, we offer you the appropriate multiwell plate for every experiment. Numbered wells and a rounded corner in the cover reduce misplacement and facilitate identification. The treatment of surfaces allows adherent cells a uniform and even cell adhesion.

• Evaporation is reduced by condensation rings
• Easily stackable
• Individually wrapped in paper/plastic blister pack
• Surface is treated with vacuum gas plasma
• Sterilized by radiation (SAL 10^-6)

Accessories Cell scrapers and cell lifters, sterile Alternative Cell culture dishes, treated, sterile, PS Alternative Cell culture flasks, treated, sterile, PS

The LABSOLUTE® filtration units are ideal for filtration of buffer solutions, cell culture media, microbiological media and other biological fluids. The PES membrane guarantees good flow through times and low protein binding.

• High traceability thanks to expiry date and Lot. no. on each filter
• 0.2 μm: Ideal for sterilization applications
• 0.45 μm: Ideal for clarifying buffer solutions and nutrient media preparation
• Filtrate bottle with lid for convenient storage of the filtered solution
• Individually packaged
• Different volumes available
• Pyrogen free and not cytotoxic
• Quick connection for hoses with an inside diameter of 8 to 12 mm
• Gamma sterilized (SAL 10^-6)

The filter funnels from LABSOLUTE® can be easily screwed onto all standard bottles with a 45 mm thread. The membrane type and pore size are imprinted for easy identification.

Accessories Filtration units, sterile, PES Accessories Filtrate bottle, sterile, PS

The LABSOLUTE® filtration units are ideal for filtration of buffer solutions, cell culture media, microbiological media and other biological fluids. The PES membrane guarantees good flow through times and low protein binding.

• High traceability thanks to expiry date and Lot. no. on each filter
• 0.2 μm: Ideal for sterilization applications
• 0.45 μm: Ideal for clarifying buffer solutions and nutrient media preparation
• Filtrate bottle with lid for convenient storage of the filtered solution
• Individually packaged
• Different volumes available
• Pyrogen free and not cytotoxic
• Quick connection for hoses with an inside diameter of 8 to 12 mm
• Gamma sterilized (SAL 10^-6)

LABSOLUTE® filtrate bottle with ergonomic design for storing sterile solutions made of PS with a PE cap.

Accessories Filtration units, sterile, PES Accessories Filter funnel, sterile, PES

Cell strainers are especially suited for the generation of homogenous single cell suspensions from tissue or primary cell lysates

• Available in 3 mesh sizes, 40 µm: 70 µm, and 100 µm
• Colour coded mesh size
• Designed to fit into 50 ml tubes
• Individually packaged
• Handle and mantle surface for improved aseptic handling
• Nylon mesh
• Pyrogen-free

PC bottle with leakproof PP screw cap. With moulded graduations and pouring rim. Ideal for storing aqueous solutions and for the short-term storage of cell culture media. Autoclavable.

Cell culture flasks with Nunclon™Δ. Surface modifications are certified pyrogen-free and tested for monolayer formation and cloning efficiency with primary and immortalized cell lines. Sterile. Bottleneck at an angle. Sterile.
Material flasks: Polystyrene
Material caps: HDPE

Accessories Cell Scrapers, sterile Accessories Caps for Cell Culture Flasks EasYFlask™, HDPE

In the case of concentration determinations in microplates, the measurement is made vertically and the layer thickness of the sample to be measured is dependent on the sample volume. Even with smaller sample volumes, the resulting layer thicknesses are sufficient for precise measurement. At a constant sample volume, concentrations can be determined with the aid of a calibration curve.

Properties:

• Optical window down to 230 nm ideal for nucleic acid determinations at 260 nm/280 nm
• For measurements of protein concentration at 280 nm
• Transmission measurements with UV light up to 230 nm
• DMSO-resistant
• Stored at -20 °C without any problem

These standard surface treated cell culture plates for the cultivation of adherent cells exhibit a reduced evaporation due to raised well rims and uniform rings on the lids.

• Available with 4-, 6-, 12-, 24-, 48-, 96- or 384-wells (lid included)
• Flat well bottom
• 96-well plate also available as round bottom plate (6.267 171)
• Single position lid reduces the risks of cross-contaminations
• Wells are labeled with alphanumeric code for easy identification
• Sterilized by gamma irradiation
• Pyrogen-free
• Material: Polystyrene

Chamber slides eliminate the need for cell transfer prior to visualisation / staining. Upper structure can be removed when culturing is complete. Useful for viral and mycoplasma testing, chromosome studies, toxicity tests and immunocytology. Sterile. The chemically coated growth surface of the Lab-Tek™II-CC² Chamber Slide System mimics polylysine and provides binding sites optimal for fastidious cells (e.g. neurons).
Material chamber: Polystyrene

Lab-Tek™: Sealant: Non-toxic medical grade silicone.
Lab-Tek II™: Sealant, biocompatible acrylic adhesive.
Lab-Tek II - CC²: modified glass slide, chemically coated to mimic polylysine.

For cell culture, Greiner Bio-One offers standard and filter cap cell culture flasks with growth areas of four different sizes. Suspension culture flasks complete the range in another sizes.They are made of high-grade polystyrene. The special design of our cell culture flasks makes it possible to efficiently reach the cell lawn with a cell scraper, whilst still providing optimal access with a pipette. The special neck
design reduces the risk of wetting the inside of the cap with medium, thus providing additional protection against contamination. The stacking rim on the top of the flasks ensures firm standing and easy stackability in the incubator.

Properties:

• Improved cell adhesion through physical surface treatment
• Cell culture flasks with filter screw cap
• Canted neck
• Graduation on both sides
• Sterile and user-friendly packaging
• 25 cm², 75 cm² and 175 cm² growth areas
• High and flat design of the 175 cm² cell culture flask for variability of media volume

4- to 48-well multidishes with Nunclon™Δ treated surface. 6-well plates also available with Poly-D-Lysine and Collagen I coating.
Raised well rims lower the risk of cross contamination.
Excellent optical quality. All available surface modifications are certified pyrogen-free and tested for both monolayer
formation and cloning efficiency employing primary cells as well as repeating cell lines. Sterile. With lid
Material dishes and lids: Polystyrene

ThinCert™ Cell Culture Inserts for advanced cell and tissue culture applications. They are compatible with standard CELLSTAR® multiwell plates. The coupling between the housing and the membrane is achieved using an automated process which produces an extremely strong and robust seal without compromising or weakening the membrane in any way. The membranes undergo a physical surface treatment to optimise cellular adherence and growth characteristics. Due to a high chemical resistance to solvents a broad spectrum of cell fixation protocols can be performed. The specific hanging geometry design of the ThinCertTM cell culture inserts ensures that there is always a gap between the membrane support and the bottom of the well.

Proporties:

• Stable clear polystyrene housing, USP class VI certified and cell culture compatible
• Hanging geometry
• Sealed PET capillary pore membrane
• Single, sterile blister packing
• Improved cell adhesion through physical surface treatment
• Simplified pipetting due to self-lift geometry
• Enhanced pipetting access and gas exchange

Clear, white or black plates with excellent optical clarity and flat bottom wells. Nunclon™Δ, MaxiSorp™, PolySorp™, Collagen I, Poly-D-Lysine surface modifications suitable for cell culture assays.
Material plates: Polystyrene
Total volume µl/well: 400

Nunclon™Δ cell culture flasks with surface areas from 25 to 175cm² are available with filter caps or Vent/Close caps for good gas exchange. Short, wide neck (angled or straight) allows easy access to entire growth surface. The Nunclon™Δ treatment is limited to the intended growth area. Neck areas are not treated to prevent cell attachment and growth in undesired areas. Certified pyrogen-free and tested for both monolayer formation and cloning efficiency employing primary cells as well as repeating cell lines. Sterile.
Material flasks: Polystyrene
Material caps: HDPE

Accessories Caps for bottles Nunclon™Δ, HDPE Accessories Cell Scrapers, sterile Alternative Cell Culture Flasks EasYFlask™, treated, PS/HDPE, sterile

Minimize plate evaporation through the uniquely engineered design of Thermo Scientific™ Nunc™ Edge-96 Well Plate. The edge plate is engineered for optical superiority allowing your automated imaging systems to operate efficiently. The plates are made of clear polystyrene for excellent optical properties making it ideal for optical measurements
Features:

• Reduced Edge Effect virtually eliminates evaporation in lengthy incubations
• Overall plate evaporation rate reduced to <2% after seven days of incubation
• Low evaporation permits full-plate results
• Large evaporative buffer zones eliminate well-to-well variability
• Perimeter moat can be filled with 1.7mL sterile H2O or media
• Low background fluorescence, ideal for optical instruments
• Ideal for image-based high content applications like measuring primary neuron cultures
• Customized bar coding available (upon request)
• Untreated or cell culture treated hydrophilic surface (Nunclon™ Δ)

Material: Polysytrene
Total well volume: 400µL

With blue vented closure, the chance for cross contamination is reduced. Ideal for shaker and suspension cell culture, media preparation or storage. Made of light, crystal clear PETG. Molded-in graduations. Leak-proof HDPE closure has a hydrophobic 0.2µm PTFE membrane that allows sterile air exchange. Flasks offer a 5-year shelf life, a 10^-6 SAL, are non-pyrogenic and non-cytotoxic. Available with flat or baffled bottom. Individually packaged for easy storage and handling.

Optimizing spheroid formation

• Allows cells to grow in suspension with virtually no cell attachment
• Supports many different cell types and their ability to generate spheroids
• Enables consistent, repeatable cell growth

Growing 3D cancer cultures

• Minimizes variability and supports the consistent formation of cancer spheroids that simulate the 3D structures of tumor growth, providing a better model system for studying tumor cell progression and efficacy of anticancer agents in vitro

Promoting formation of embryoid bodies (EB)

• Demonstrates superior quality for embryoid body formation of pluripotent stem cells with minimal spontaneous differentiation
• Resultant cells are able to differentiate into all three germ layers

Certifications/Compliance

• Free of animal products
• Pyrogen-free: Endotoxin tested (0.5 EU/mL)
• Biologically inert: USP biological reactivity class VI
• High level of sterility: SAL 10-6, ethylene oxide gas sterilization
• Shelf Life 4 years

• Nunclon™Δ surface for cell culture
• Non-treated plates are optimised for scintillation counting
• White or black upper structure with polymer bottom plates combine the optical clarity of virgin crystalline polystyrene with optimal surface for a wide range of HTS applications
• Working volume range: 50 - 200µl/well

Accessories Sealing Tapes for MultiWell Plates