Cell culture media and reagents

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Dimethyl sulfoxide, ACS, suitable for cell culture (min. 99.9 %)

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 RPMI 1640 MEDIUM

BioWest

RPMI are general purpose enriched media with extensive applications for a broad spectrum of mammalian and hybridoma cells, including human myeloma, mouse hybridoma, human leukocytes, and B and T lymphocytes. It was originally formulated for suspension cultures and monolayer culture of human leukemia cells....

 DULBECCO’S MODIFIED EAGLE MEDIUM - DMEM

BioWest

The DMEM medium is a modification of the BME which contains a higher concentration of amino acids, vitamins and other additives. DMEM is for supporting and maintaining a vast range of mammalian cell types. There are two types of DMEM: one with a high glucose content (4.5g/L), and the other with a low glucose content (1.0g/L). The DMEM low glucose has been developed for the culture of mouse embryonic cells....

 MEM W/ EARLE’S SALTS

BioWest

Minimum Essential Medium (MEM) with Earle’s Balanced Salts is a modification of Eagle’s earlier Basal Medium (BME) which contains a higher concentration of essential nutrients. These media promote the growth of a variety of normal and transformed cells. Since they contain Earle’s Balanced Salts, they are suitable for use in atmospheres charged with CO2 gas....

 MEM W/ HANKS’ SALTS

BioWest

Minimum Essential Medium (MEM) is a modification of Eagle’s earlier medium Basal Medium Eagle (BME). This MEM w/ Hanks’ Salts formulation contains Hank’s salts for use without CO2....

 Antibiotica

BioWest

Protect your cell lines from contamination by foreign organisms such as bacteria, fungi and viruses and/or use the antibiotics for selection after transfection.
Most antibiotics suppress the growth of micro-organisms by blocking an anabolic pathway.
Nanomycopulitine actively kills bacteria, including mycoplasma, in all three stages of development without interfering with the eukaryotic metabolism....

 HAM’S F12 / F-12 NUTRIENT MEDIUM

BioWest

Ham's F12 was originally developed for serum-free clonal growth of Chinese hamster ovary (CHO) cells, lung cells and mouse L-cells. It is the medium of choice for supporting cells from rodents (especially rabbit and rat) and has proven to be an excellent
cloning medium for the culture of myeloma and hybrid cells (hybridomas)....

 DMEM - HAM’S F12

BioWest

The DMEM Ham’s F12 offers excellent performance for certain epithelial, endothelial and granulose cell types. With proper supplementation, it is a highly successful basic media for serum free cell culture....

 ISCOVE’S MODIFIED DULBECCO’S MEDIUM - IMDM

BioWest

Iscoves media are enriched modifications of DMEM containing sodium selenite. They are excellent for rapidly proliferating high-density cell cultures. The addition of BSA, purified human transferring and soybean lecithin creates a serum free condition ideal for supporting B and T lymphocytes. IMDM was the first media utilizing HEPES buffer. Other cell types can be cultured using this medium under serum free or reduced serum conditions....

 Basal medium eagle - BME

BioWest

BME ist der Vorgänger von Eagle Minimum Essential Medium (MEM) und Dulbecco Modified Eagle Medium (D-MEM). Es ist das einfachste der Basismedien mit allen wesentlichen Komponenten für das Zellwachstum. Basalmedium Eagle (BME) begünstigt idealerweise Zelllinien wie HeLa, L-Zellen und primäre Säugetierfibroblasten....

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