Microbiology (Cultivation)

DURAN^®. Roux type. With fire-polished, offset neck. Glass Type I / neutral glass as per USP, EP and JP. Autoclavable.

Transparent. With baffles indented into the base which increases mixing when trypsinizing and aerating in processes such as cell culture and fermentation. Autoclavable. 500 ml and 1000 ml flasks have a straight neck, suitable for 38 mm standard caps of stainless steel, aluminium or plastic.

Anodised in a choice of colours. For sterile, but not hermetically sealing, closure of containers. With nichrome steel springs that clamp onto the outer wall of the container. Autoclavable.
Cleaning advice available on request.

The STERI second sterilisers permit instant disinfection and sterilization of all small instruments directly at the workplace. Especially suitable for laminar flow cabinets. Biological investigations and tests prove that the 250 °C temperature maintained in the glass bead container guarantees complete destruction of all micro-organisms and spores within a few seconds. The one part of the instrument which is inserted into the STERI glass beads is sterilized. The STERI 250 and 350 are two versions of the same quality apparatus, with a stainless steel container, high-performance heating element, reduced heating time, high precision thermostat, temperature limiter and built-in thermometer.

• For perfect hygiene directly at the workplace
• A guaranteed Swiss quality product

STERI 250: Steriliser incl. plain metal cover, glass-cup and glassbeads (150 g). Suitable for small & medium sized instruments (scissors, tweezers, forceps, knives, blades, spatulas, etc.).
STERI 350: Steriliser incl. plain metal cover and glassbeads (300 g). Especially suitable for medium sized and longer instruments.

For the cultivation of anaerobic and microaerophilic microorganisms in a defined and rapidly generated gas atmosphere.The requested atmosphere may be reached under ideal conditions by two methods. Either by using chemical gas packs (anaerobe systems) or by manually evacuating the jars with a vacuum pump and flushing with gas afterwards (e.g. with nitrogen), in this case no chemical accessories are needed (except anaerobic jar eco and crystal eco). The jars are made of robust stainless steel or transparent PC.The lids are made of UV-resistant plastic or transparent polycarbonate with two corner valves incl. tube clips for vacuum hoses (5 mm i.d.) and with manometer for exact control of the vacuum or overpressure from -1 to 0.2 bar (except anaerobic jar eco and crystal eco). The optional racks are made of stainless steel providing holders for comfortable operation of the anaerobe systems.

Methods for reaching anaerobic conditions: 3 x times evacuating and filling gas, flushing with gas for 5 minutes and chemical gas production (GasPacks) for Anaerobic jars "small", "standard", "large" and "crystal". For Anaerobic jar "eco" flushing with gas for 5 minutes (Using GasPack-Kits).

Customized: Other equipment available upon request. Examples: customized lids, e.g. made of special or heat-resistant materials e.g. stainless steel, heat-resistant pressure gauges (up to 100 °C), also without cocks / manometer available.

Accessories Accessories for anaerobic jars

For microbiological quality control. Quick connectors at both sides of the manifolds enable fast connections of either tubing, another manifold or end caps. Various funnel and filtration units are available.

• Full autoclavability without disassembly
• Sterile venting
• Reduced risk of secondary contamination
• Low working height
• Rubber feets providing better stability at the workplace

Scope of supply:
Microsart^® Manifolds for Microsart^® funnels and filters: Manifold, filter base support with sinter frit, blind coupling, 20 cm tubing and venting filter
Microsart^® Manifolds for Biosart^® 100 monitors: Manifold, filter base support with sinter frit, Biosart^® adapter, blind coupling, 20 cm tubing and venting filter
Microsart^® Manifolds for Biosart^® 250 funnels: Manifold, filter base support with sinter frit, blind coupling, 20 cm tubing and plug for venting holes
Microsart^® Manifolds including stainless steel funnels (100 / 500 ml): Manifold, filter base support with sinter frit, blind coupling, 20 cm tubing and plug for venting holes

Cell culture multiwell plates are available in the hydrophilic surface (TC surface treatment) for improved cell adhesion and hydrophobic surface for suspension cultures and hybridoma cells. Cell culture microplates from the CELLSTAR® range are specifically treated. This treatment leads to polar groups such as carboxyl and hydroxyl groups , added to the plastic surface. These allow the growth of adherent cell lines. They are also suitable for automated systems and contain an alphanumeric well coding. They are also suitable for footprint systems and contain an alphanumeric well coding.

Properties:

• High clarity enables optimal microscopic examinations
• Lid enables optimal gas exchange with the lowest possible evaporation
• Stackable
• Alphanumeric well coding
• Individually wrapped peel-off bags
• Consecutive lot numbering
• 384 and 1536 well foramt available

This agaroses are suitable for routine analysis of nucleic acids. They combine tensile strength with low electroendoosmotic effects to achieve high resolving power for a wide size range of nucleic acid fragments. AG agaroses are also very environmentally friendly, being manufactured by a process which excludes organic solvents. Also available as low melting point agarose for nucleic acid recovery and enzymatic applications (Cat. No. 4.658 455) and as High Resolution PCR grade agarose for separation of small DNA fragments of up to 800 bp (Cat. No. 6.270 615). Other packing sizes on request.

Specifications	AG | LMA | HRA
CAS: Appearance:	9012-36-6 | 39346-81-1 | 39346-81-1 White to off-white powder
EEO:	≤ 0,13 % | ≤ 0,1 % | ≤ 0,1%
Gelling Point:	36 °C ±1,5°C (1,5 % gel) | 26-30 °C (1,5 % gel) | ≤ 33 °C (1,5 % gel)
Melting Point:	88 °C ±1,5°C (1,5 % gel) | ≤ 65 °C (1,5 % gel) | ≤ 70 °C (1,5 % gel)
Solubility:	Clear, colourless solution at 1g in 100ml water
Moisture:	< 10 %
Gel Strength:	≥ 1200 g/cm2 (1% gel) | ≥ 200 g/cm² (1% gel) | ≥ 750 g/cm² (1,5% gel)
Sulphate:	< 0,20 % | ≤ 0,1 % | ≤ 0,1 %
DNase, RNase, Protease, Endonuclease:	None Detected