Cultivation

The CELLCOAT® product line comprises cell culture vessels which are coated with proteins of the extracellular matrix or synthetic proteins. Beside an improved adhesion and proliferation of primary cells and various cell lines. CELLCOAT® plates are highly suitable for serum-free and serum-reduced cell cultivation. Experiments which include additional washing steps or stressful procedures, e.g. transfection. Moreover, the differentiation of individual cell types can be enhanced through the protein-coating.
Poly-L-Lysin (PLL are synthetic molecules that are used to improve adhesion of different cell types to polystyrene surfaces. Especially when serumfree or serum-reduced medium is used or when experiments such as transfections are performed, the cultivation efficiency of individual cell lines can be improved. As synthetic molecule Poly-L-Lysine is free of impurities carried by other proteins.

Proporties:

• Shelf life Multiwell Plates: 24 months at room temperature
• Shelf life Microplates: 18 months at room temperature
• Cell differentiation and neuron growth
• Automated cell culture

Roller Bottles InVitro
For industrial scale production of vaccines, monoclonal antibodies or pharmaceuticals. In vitro Roller Bottles molded of durable PETG. Excellent substrate for adherent cells. Quick-action ergonomic closure reduces wrist stain and increases productivity. Wide range of sizes with surface areas from 1050cm² to 4200cm². Available in both Standard and the patented Expanded Surface (XPS), which enables increased cell growth and product yield without the need to purchase additional production equipment or increase labor. In Vitro XPS Roller Bottles have specially designed pleats that dramatically expand the available growth surface. The pleats are oriented in the direction of rotation to minimise turbulence. Flat panels between sections allow microscopic viewing and expedite drainage during medium replacement and cell harvest. Easy-to-read graduations for medium fills. Lot number is printed on each bottle to maximise traceability. Sterile.
Roller Bottles TufRol™ and TufRol™ EZ
TufRol™ Roller Bottles enable to scale cell culture processes from plates, to flasks, to roller bottles. They can be used as the primary production vessel or as a mid-scale expansion platform to feed Cell Factories or Suspension Culture Bioreactors. One-piece, seamless design reduces possibility of leaking. TufRol™ EZ bottles with shallow or deep indents make them excellent for manual and automated handling. Proprietary manufacturing process for greater impact resistance. Vertical expanded surfaces version facilitates emptying and reduces retention of product. Certified non-cytotoxic, pyrogen-free and sterile (10^-3 SAL). Validation Binder available.

For carrying out germ resistance tests. All papers are made from high-purity cellulose and are free of spores, bacteria and toxic substances that could influence the germination of the seeds. The highly absorbent papers store water for the entire duration of the tests. All germ test papers meet the requirements of ISTA and AOSA.

• Reliable seed testing
• Conductivity 40 mS/m
• pH value between 6.0 and 7.5
• High absorbance
• Roots do not grow through the paper
• Papers conform to ISTA recommendations of 2017

ThinCert™ Cell Culture Inserts for advanced cell and tissue culture applications. They are compatible with standard CELLSTAR® multiwell plates. The coupling between the housing and the membrane is achieved using an automated process which produces an extremely strong and robust seal without compromising or weakening the membrane in any way. The membranes undergo a physical surface treatment to optimise cellular adherence and growth characteristics. Due to a high chemical resistance to solvents a broad spectrum of cell fixation protocols can be performed. The specific hanging geometry design of the ThinCertTM cell culture inserts ensures that there is always a gap between the membrane support and the bottom of the well.

Proporties:

• Stable clear polystyrene housing, USP class VI certified and cell culture compatible
• Hanging geometry
• Sealed PET capillary pore membrane
• Single, sterile blister packing
• Improved cell adhesion through physical surface treatment
• Simplified pipetting due to self-lift geometry
• Enhanced pipetting access and gas exchange

Falcon^® Cell Culture Flasks feature a unique vacuum-gas plasma tissue culture treatment. These products are certified as sterile, non-cytotoxic, and pyrogen-free. Other surface media available on request.

PMP with PP screw closure. Transparent. Chemical-resistant. Impact-resistant. Autoclavable. Permeable to microwaves. Screw off closure before autoclaving.

Cell culture multiwell plates are available in the hydrophilic surface (TC surface treatment) for improved cell adhesion and hydrophobic surface for suspension cultures and hybridoma cells. Cell culture microplates from the CELLSTAR® range are specifically treated. This treatment leads to polar groups such as carboxyl and hydroxyl groups , added to the plastic surface. These allow the growth of adherent cell lines. They are also suitable for automated systems and contain an alphanumeric well coding. They are also suitable for footprint systems and contain an alphanumeric well coding.

Properties:

• Lid enables gas exchange with the lowest possible evaporation
• High clarity of the clear microplates for optimal microscopic examinations
• Stackable
• Alphanumeric well coding
• Consecutive lot numbering
• 96 and 1536 well format available

C-Flex^® is a patented thermoplastic elastomer tubing specifically designed to meet the critical demands of pharmaceutical and biopharmaceutical applications for fluid processing.

C-Flex^® tubing for Biopharma Manufacturing is specifically designed for aseptic welding connections and sealing disconnection. C-Flex^® is suitable for sterilization by gamma irradiation and autoclave. It is moldable, bondable and formable, ideal for single-use assemblies, such as tubing with bags manifolds.

Features/Benefits:

• Sealable and weldable either pre- or post- sterilization
• Sterilizable by gamma irradiation and autoclave
• Complies with USP Class VI and ISO standards
• Full Validation Package available for all C-Flex commercial formulations.
• Moldable, bondable and formable for single use assemblies and overmolds
• Pyrogen-free, non-cytotoxic, non-hemolytic
• Remains flexible from -45°C to 135°C
• Significantly less permeable than silicone
• Smooth inner bore for superior fluid flow
• Safe disposable through incineration
• All formulas are Animal-Derived Component Free (ADCF)

• Aseptic sealing disconnections
• Aseptic welding connections
• Sterile dosing and filling
• Buffer and media preparation
• Cell culture operations
• Purification operations
• Diagnostics products
• Biopharma manufacturing
• Single-use fluid transfer sets

Non-binding surfaces from Greiner Bio-One are achieved through a stable chemical modification to covalently link functional groups with the base polystyrene polymer. Under aqueous assay conditions a hydrate layer forms, preventing dissolved biomolecules from binding to the microplate surface. As the non-binding surface is stable under common assay conditions, there is no potential for degradation or leaching and resultant assay interference.

Properties:

• Äußerst geringe, nicht-spezifische Biomolekülbindung (Proteine, DNA, RNA)
• Langfristige Oberflächenbeständigkeit ohne Degeneration oder Auswaschen
• Hohe Testsensitivität mit reduziertem Hintergrund

Cell Culture Plates with lid, condensation rings, and deep-well design for reduced contamination and evaporation. Reliable vacuum-gas plasma tissue culture treatment provides well-to-well and plate-to-plate consistency.

• TC-treated polystyrene
• With special low evaporation lid
• Flat bottom
• Pyrogen-free
• Individually wrapped