Cell culture media and reagents

Ham's F12 was originally developed for serum-free clonal growth of Chinese hamster ovary (CHO) cells, lung cells and mouse L-cells. It is the medium of choice for supporting cells from rodents (especially rabbit and rat) and has proven to be an excellent
cloning medium for the culture of myeloma and hybrid cells (hybridomas).

This complex medium was developed specifically for nutritional research of chicken
fibroblasts. Today, 199 media are widely used for the maintenance of non-transformed cells, vaccine and virus production and primary explants of epithelial cells. The media can be formulated either with Earle salts or Hanks Salts. The media formulated with the Earle salts are buffered with a bicarbonate / carbonic acid solution and retain their pH in a CO2 incubator. The use of the Earle salts under ambient conditions results in a rapid rise in the pH of the culture medium.
The media formulated with the Hanks salts are buffered with saline solutions designed for balancing in ambient conditions, and their use in a CO2 incubator results in a rapid drop in the pH of the culture medium.

CMRL-Medien sind besonders für das klonen von Affen Nierenzellen und für das Wachstum vieler anderer Säugetierzelllinien geeignet, wenn sie mit Pferde- oder Kälberserum ergänzt werden.

Glascow Minimum Essential Medium was originally developed by Ian MacPherson and Michael Stoker as a modification of Eagle’s medium (BME). The modifications included adding 10% tryptose phosphate and twice the normal concentration of amino acids and vitamins. This medium was used to study the genetic factors affecting cell competence.

Ham's F10 ist ein beliebtes Medium für das Wachstum von anspruchsvollen Zelllinien.

Ham’s F14 medium was developed from the Ham’s F12, which was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. Ham’s F14 contains a double concentration of amino acids compared to the Ham’s F12. This product is also supplemented with Calcium Chloride and Ascorbic Acid.

The Leibovitz L-15 media were formulated to promote the cell growth in
medium not balanced in CO2. The formulations were developed with the sodium
bicarbonate buffer. The Leibovitz L-15 media are buffered by a complement of salts,
free base amino acids and galactose, so they can be used under conditions of free
gaseous exchange with the atmosphere. When properly supplemented, L-15 Medium supports established cell lines, such as HEp-2 and LLC-MK2, as well as primary explants of embryonic and adult human.

These media were formulated as a reduced serum supplemented medium for the cultivation of human microvascular endothelials cells. For other microvascular cells, it is recommended to be used with growth factors, like EGF or hormones like hydorcortisone.

MEM á is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, thioctic acid, vitamin B12, biotin, and ascorbic acid. MEM á - Modification can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells.