PCR

The Alpha Cycler family form PCRmax provides a range of features which are specifically designed to meet the needs of modern researchers when amplifying DNA. Given the flexibility in format and block options (96 or 384 well) scaleability is core in the Alpha Cycler range. Alpha Cycler software has features such as recently used programmes, allowing users to quickly access their most commonly used protocols without the need to navigate through folders to find it, individual user logins, with protected protocols. The Alpha Cycler systems also contain a novel programme wizard which allows users to define a protocol based specifically on their primer sequence and template source. Primer sequences or T_M"s are entered, amplicon length and source defined and the on board algorithm generates an optimised protocol for your assay. The Alpha Cyclers range of block options allow for use of the most commonly used consumables from different manufacturers and includes 96 well plates (0.2 ml), tubes (0.2 ml), strip tubes (0.2 ml) and 384 well plates.

• Single, dual or four block options available with either 96 or 384 well gradient blocks
• Multi-block units have totally independent blocks for maximum flexibility
• HD Android driven touchscreen interface for simplified programming
• Generate and send instrument reports to diagnose the health of your system and identify any issues before they impact users work
• Lock programs with user defined password
• Alpha Track mobile app allows remote monitoring of run progress

Alpha Track App (free of charge): Track the progress of runs and health of your system remotely via the mobile app
The Alpha Track Application is used in conjunction with your Alpha Cycler and allows users to:

• Remotely track the progress of runs
• Send a health report for diagnosis by the technical support team
• Be notified of any issues and prevent system failures before occurring

Specifications
Block temperature:	10 to 100 °C
Block uniformity at 55 °C:	±0.3 °C
Temperature accuracy at 55 °C:	±0.25 °C
Max. Heating rate:	3.4 °C/s

This simple to program PCR thermocycler unit is compact in design, programming is intuitive with the large display and multiple pre-programmed templates supplied with the unit. The OptiMax comes with standard built in 2-step, temperature optimization, touchdown and time increment protocols that are easy to adjust to meet cycling needs. Select lid temperature or turn it off.

• Flexible Programming with FlexTemp™ technology
• Fast ramp rates 5 °C heating / 3.5 °C cooling
• No condensation after overnight cooling at 4 °C
• Individual temperature setting for each block
• Password protected programs
• Simple user interface
• With USB and RS232 interface

Specifications:
Sample capacity:	1 x 96 well plate, 12 x strip tubes (8 x 0.2 ml), 96 x 0.2 ml tubes
Programmable temperature range:	4 °C ... 99.9 °C
Temperature control:	Calculated or block
Temperature accuracy/uniformity:	±0.5 °C/±0.5 °C
Heating/cooling method:	Peltier
Max. heating/cooling rate:	±5 °C/±3.5 °C per second
Temperature range of 6-segment blocks:	30 °C ... 99 °C (can be set independently)
Maximum temperature difference between 6-segment blocks:	±24 °C
Programmable lid temperature:	60 °C ... 65 °C, 100 °C ... 115 °C
Program memory: User program folders:	200 complete programs 50 sets
Dimensions (W x D x H):	240 x 420 x 250 mm
Weight:	9 kg
Supply requirements:	230 V, 50/60 Hz*

Mid-size thermocyclers - find space on any laboratory bench

• Approved cycler with new design
• With and without gradient function
• Gradient upgrade from ³PRIMEX to ³PRIMEG
• 3.5" colour touch screen with graphical display
• Height-adjustable and heatable lid, temperature from 100°C to 115°C, with ON/OFF switch and automatic switch off against overheating
• Password protected
• Auto-Restart
• USB port
• 4 years warranty for the Prime Thermocycler

Specifications
Block temperature:	+4°C to +100°C
Temperature gradient (only 3PRIMEG or 3PRIMEX with upgrade):	+30°C to +80°C
Max. Gradient:	+14°C
Block uniformity at 55°C:	<±0.3°C
Max. Heating rate:	3.0°C/s
Dimensions (W x D x H):	210 x 350 x 180

Alternative Thermocycler Alpha Cycler

Made of highly transparent PP without additives. Ultra-thin PP ensures optimum heat transfer during amplification. Comprehensive thermal cycler compatibility. Reaction vessels with flat lids for Real Time PCR / quantitative PCR have matted lids for easier labeling.

Features & Benefits:

• Packaging labeling with production symbols for easier identification and storage
• Free of detectable DNase, RNase and human DNA
• Free of detectable endotoxins

For cell culture applications, sample preparation and storage of samples and solutions.

• Conical
• Temperature resistant from -86 °C to 100 °C

Accessories Eppendorf Tube Racks, PP Accessories Accessories for Eppendorf Tubes® 25 ml, PP Alternative  Alternative Eppendorf Tubes®, PP, amber, with screw cap, HDPE Alternative  Alternative Eppendorf Tubes® 25 ml, PP, starter pack

These UV sterilisation cabinets provide a convenient area for setting up PCR reactions in a nucleic acid free environment thus limiting PCR reaction contamination. Acting effectively as a low cost alternative to a Cleanroom, the powerful UV lights on the cabinets denature nucleic acids in 5 to 30 minutes making them unsuitable for amplification. The cabinet incorporates safety features to prevent user exposure to UV light. The UV lights themselves are timer controlled and there are safety switches on the cabinets doors which turn off the UV lights when opened. Constructed from 10 mm acrylic, the cabinets also act as efficient shields from beta radiation emissions and can therefore be safely used with isotopes such as ³²P.

Specifications	Maxi / Midi / Mini
UV Lights:	4 x 15 W
White Lights:	15
Dimensions (H x W x D):	770 x 580 x 420 mm / 620 x 580 x 420 mm / 450 x 580 x 350 mm
Weight:	19 kg / 14.6 kg / 12 kg

Two in one mini kit for gel extraction and PCR clean up.

Sample material:	Agarose gel, PCR reaction mixture
Sample amount:	< 400 µL PCR reaction mixture, < 400 µg agarose gel
Fragment size:	50 bp−?approx. 20 kbp
Typical recovery:	70−?95 %
Elution volume:	15−?30 µl
Downstream application:	Cloning, PCR, Restriction analysis, Sequencing

Adhesive sealers are an interesting alternative to heat sealing systems or CapMats. They are easy to use and there is a lower risk of cross-contamination because they are used as disposable products.

Properties:

• Free of detectable DNase, RNase, human DNA
• Non-Pyrogenic
• Highly transparent film for optical measurements
• Ideally suited for protein crystallography
• Coated with a silicone adhesive
• Only sticks when the film surface is pressed on
• The film does not adhere to gloves
• Substances in powder form and biological model organisms like Drosophila melanogaster or Caenorhabditis elegans do not stick to vessels sealed with VIEWseal™
• Temperature resistant from - 80°C to + 110°C
• Suitable for PCR applications and sample storage
• Minimal autofluorescence

BRAND has significantly expanded its product range of extra-thin-wall disposable products, which were specially developed to satisfy the demands of PCR applications.

Quality features

• Suitable for use in common thermocyclers
• PP, extra-thin uniform wall thicknesses to provide the optimal thermal transfer and short cycle times
• 96-well PCR plates with blue alphanumeric code and cut corner marking
• Highly clear flat covers optimally suited for qPCR
• DNase-, DNA- and RNase-free
• Autoclavable at 121°C (2 bar), acc. DIN EN 285
• qPCR: Products which are suitable for Real Time PCR (qPCR)

White PCR products offer significantly better results for qPCR, and are specifically recommended by many thermocycler manufacturers.
For PCR-tubes with detached cap strips.

Accessories PCR cap tool, PA

The use of the 96 well format allows the scale up of basic PCR work, while the 384 well format is ideal for high-throughput screening projects.

Properties:

• Ultra thin polypropylene for optimal heat transfer from the thermoblock to the reaction solution
• Free of detectable DNase, RNase, human DNA
• Non-Pyrogenic
• Sealable with sealers SILVERseal™ , VIEWseal™ and AMPLIseal™ or with compatible 8-cap strips
• For Research Use Only, not for use in diagnostic procedures, unless otherwise indicated

DNA LoBind plates for maximum sample recovery from nucleic acids by reducing the binding of the sample to the surface. Suitable for preparation or storage of DNA and RNA samples, forensic trace analysis, preparation of dilution series in quantitative qPCR, sample preparation in next-generation sequencing and creation of genome or oligonucleotide libraries.

• PCR clean: free of human DNA, DNase, RNase and PCR inhibitors
• High-contrast, alphanumeric lettering
• Frame white or blue
• Rounded RecoverMax^®-Well-Design
• Elevated well frames for reliable sealing

Alternative

Stable, flexible protection of samples during short-term storage.

The Fast PCR tube strips made of polyethylene transfer heat much better and faster than tubes made of traditional plastics. They are particularly suitable for use with Fast PCR reagent kits and for cyclers with high heating and cooling rates. Under these conditions, the following results higher yield and significant time savings.

• Suitable for PCR, Fast PCR and Real Time PCR
• Increased thermal conductivity through PE tubes
• Higher yield and significant time savings
• Can be used in any cycler for 100 µL volumes
• Lid strip PP
• With flat or domed lid
• Certified PCR clean

Alternative

The Mastercycler X50 family offers tempering rates of up to 10 °C/s and is the ideal cycler family for routine applications as well as state-of-the-art molecular biological research. The Mastercycler X50 family consists of units with user-friendly VisioNizeR touchscreen control panel or eco units, which are externally controlled. A low noise level, low energy consumption and the versatile flexlidR concept round off the functions of this powerful and convenient PCR cycler.

Features & benefits:

• Networkable with nine other eco devices
• With CycleManager X50 as central control unit, up to 50 eco devices can be connected to one computer or laptop and each one can be controlled and monitored individually and independently
• The intuitive interface manages who has access to the machine and allows you to set user access levels

For measuring absorbance assays in the visible and UV range, fluorescence and chemiluminescence assays, DNA- and protein concentration with absorbance or fluorescent dyes, cell based assays, cell viability and apoptosis assays. UV-VIS Microplates feature an ultrathin film bottom for good light transmission in the UV range. Black Eppendorf Microplates offer an good signal-to-noise ratio. White Eppendorf Microplates are optimized for highest sensitivity in the detection of luminescence signals by maximizing reflection.

Alternative

Suitable for sample storage and preparation, protein analysis and genotyping.

• High-contrast, alphanumeric lettering
• Wells transparent with white frame
• Rounded RecoverMax^®-Well-Design
• Elevated well frames for reliable sealing, even with heat sealing
• Temperaturresistance from -86 °C to 100 °C
• Autoclavable (121 °C, 20 min)
• Centrifugation resistance up to 6000 × g
• PCR clean: free of human DNA, DNase, RNase and PCR inhibitors or sterile

Alternative

DNA LoBind plates for maximum sample recovery from nucleic acids by reducing the binding of the sample to the surface. Suitable for preparation or storage of DNA and RNA samples, forensic trace analysis, preparation of dilution series in quantitative qPCR, sample preparation in next-generation sequencing and creation of genome or oligonucleotide libraries.

• V-bottom wells
• PCR clean: free of human DNA, DNase, RNase and PCR inhibitors
• High-contrast, alphanumeric lettering
• Wells transparent with white frame
• Rounded RecoverMax^®-Well-Design
• Elevated well frames for reliable sealing

Alternative

Protein LoBind plates are specially designed for proteomics and other areas of protein research. Suitable for preparation or storage of protein, peptide or antibody samples, enzymatic assays, storage of virus stock solutions or cell suspensions and sample processing in toxicology.

• V-bottom wells
• High-contrast, alphanumeric lettering
• Wells transparent with white frame
• Rounded RecoverMax^®-Well-Design
• Elevated well frames for reliable sealing
• PCR clean: free of human DNA, DNase, RNase and PCR inhibitors

Alternative

Fully compatible to all current standard-equipments (reading and washing devices). Safe stacking, no danger of slipping. Appropriate gripping surfaces at the side. Frosted surface of the plates prevents errors from extraneous light. Alpha-numerical well identification for easy sample location. Space for identification marking on the side surface. Covers with condensation-rings to avoid medium-losses and uneven evaporation. Lid orientation corners reduce risk of cross-contaminations.

U-Well: No edges, therefore simple and clean to pipette into, for +/- evaluations, especially suitable for agglutination-tests.
V-Well: Especially good for releasing the "last drop" from the pipette, for +/- evaluations, suitable for storage of samples.
F-Well: For precise optical measurements, for microscopic applications (Bottom Reading), rims at the bases prevent a scuffing of the optical surface, widened cell-growth-surface.

Rapid methods for the detection of mycoplasma contamination are essential for improving monitoring of the production process of biologicals. With the use of the Microsart^® Mycoplasma the testing time can be reduced from weeks to just 3 hours.

PS. Radiation-sterilized. With cover. Ideal for screening DNA libraries. Holder for membranes for dot-blotting. Vessels for denaturing, hybridization and washing stages. Useful for storing membranes. Colonies easy to recognize as material is transparent. Can be stacked safely. High and robust walls. Can be frozen down to -20 °C.
Can also be supplied with cell culture surface.

BRAND^® Premium PCR plates

• ultra-thin walled
• even and rapid heating of all samples
• reinforced plate deck
• no warping, no wiggling, for use with robots
• free of DNase, DNA, RNase and endotoxins
• suitable for qPCR

BRAND^® PCR Sealing film

• highly transparent for qPCR
• non-tacky surface
• press to seal
• convenient end tabs
• no annoying adhesive residues

Every package includes 50 PCR plates 96-well and 50 qPCR sealing films (Art. Nr. 4.000 237).

The PCR^® rack base is made from robust ABS construction, and the lid is made from PC. Versatile Rack for use on a bench top or for freezer storage.

• Storing of tubes down to -80 °C
• Holds 0.2 ml individual tubes or strips
• Ambidextrous
• Detachable lid
• Stackable

Cap strips

• strips with 8 microcaps
• easy and effective sealing of PCR plates and PCR Tube Strips
• easy to remove using extension at end of strip
• autoclavable (at 121°C for 20 min.)
• certified free of human DNA, DNase, RNase and PCR Inhibitor