PCR

BRAND has significantly expanded its product range of extra-thin-wall disposable products, which were specially developed to satisfy the demands of PCR applications.

Quality features

• Suitable for use in common thermocyclers
• PP, extra-thin uniform wall thicknesses to provide the optimal thermal transfer and short cycle times
• 96-well PCR plates with blue alphanumeric code and cut corner marking
• Highly clear flat covers optimally suited for qPCR
• DNase-, DNA- and RNase-free
• Autoclavable at 121°C (2 bar), acc. DIN EN 285
• qPCR: Products which are suitable for Real Time PCR (qPCR)

White PCR products offer significantly better results for qPCR, and are specifically recommended by many thermocycler manufacturers.
Strips of 8 or 12 connected PCR tubes (0.2 ml). Cap strips consisting of 8 or 12 domed caps (available separately) provide tight seal during all PCR cycles, thus protecting samples from cross-contamination. The tubes have a holding strap at one end, and the cover strips on each lid have a small lip on the side for careful, contamination-free opening. Consistent tube wall thickness allows rapid heat transfer along the entire exterior tube surface. Made of pure, virgin polypropylene, free from RNase-, DNase- and DNA. Autoclavable at 121 °C (2 bar), according to DIN EN 285.

Other colors on request.

Accessories PCR cap tool, PA

Ideal for sealing PCR plates.

• PCR clean: Certified free from human DNA, DNase, RNase and PCR inhibitor
• PCR Film: Allows visual monitoring of samples
• PCR Foil: Easy to pierce, no sticking of the pipette tips (ideal for automated systems)
• Heat Sealing: Position the film or the foil with the adhesive edges on the plate. Seal the plate using the heat sealer
• Sealing mats are reusable and autoclavable

Accessories

BRAND has significantly expanded its product range of extra-thin-wall disposable products, which were specially developed to satisfy the demands of PCR applications.

Quality features

• Suitable for use in common thermocyclers
• PP, extra-thin uniform wall thicknesses to provide the optimal thermal transfer and short cycle times
• 96-well PCR plates with blue alphanumeric code and cut corner marking
• Highly clear flat covers optimally suited for qPCR
• DNase-, DNA- and RNase-free
• Autoclavable at 121°C (2 bar), acc. DIN EN 285
• qPCR: Products which are suitable for Real Time PCR (qPCR)

White PCR products offer significantly better results for qPCR, and are specifically recommended by many thermocycler manufacturers.
Eight 0.2 ml PCR tubes joined together in a strip with attached, hinged 8-cap strips. PP. The hinged, domed cap makes opening and closing the tubes with one hand easier.

Bench-top models for protection against contamination during a variety of DNA/RNA procedures.

• UV surface irradiation, via 1 x 25 W 254 nm open UV lamp or 2 x 30 W open UV lamp (UVT-S-AR)
• Patented high intensity UV air cleaner, 25 m³/hour cleaner-recirculator continuous air flow with 1cm UV irradiation distance
• UVlamp in the upper hood disinfects the working area, inactivating DNA/RNA fragments during 15-30 min of exposure
• UV exposure control, 24 hour digital timer
• Convenient, easy to use digital timer for accurate control of UV exposure
• White lamp provides local illumination of the workplace to optimise visual control during operations
• Shock proof glass panels, metal framework and stainless steel work surface
• Automatic switch-off of open UV light when door opened
• UVT-B-AR is available with 1 x access port or 1 x internal power socket, UVT-S-AR with 3 x internal power socket, UVC/T-M-AR with 1 x access port

Specifications
External dimensions (W x D x H):	690 x 585 x 555 mm
Working area dimensions (W x D):	650 x 475 mm
Weight:	32 kg

Accessories

The use of real-time PCR (qPCR) is becoming increasingly widespread. The line of white PCR products by BRAND offers significantly better results in this application than transparent tubes or plates. The different products in this line are uniformly colored with TiO2 (titanium dioxide), so that in combination with the smooth surfaces, the optimum reflection of the fluorescence signal is provided.
The ultra thin-walled PCR plate design facilitates constant, rapid and precise heat transfer. The smooth vessel interior minimizes the binding of enzymes and nucleic acid to the walls. The rim of the wells are designed to protect against cross-contamination, allowing a reliable seal with the sealing mats tailored to the plates. With alphanumeric code and cut corner for easy orientation.

96-well, non-skirted
PP. 0.2 ml wells. 96-well non-skirted PCR plates are suitable for most commercially available thermocyclers.

96-well, semi-skirted
PP. 0.2 ml wells. 96-well semi-skirted PCR plates can easily be labelled or tagged with a barcode.

96-well, semi-skirted. Suitable for Roche^® LightCycler^® 480 and other thermocyclers
PP. 0.15 ml wells. Semi-skirted. Available with or without self-adhesive sealing film (polyester, high-transparent, for qPCR).

96-well, skirted
PP. 0.2 ml wells. 96-well skirted PCR plates are especially rigid, and are optimally suited for use with automatic pipetting systems and for being transported.

Alternative 384 well PCR-Plates, white, for real-time PCR

Removable hinged lid fits both sides. Flip this polypropylene reversible rack to the size of tubes you need. PCR side of the rack has 168 wells that hold 0.2ml tubes. The wells accommodate 8- or 12-tube strips or individual tubes. The opposite side of the rack has 40 wells that hold 0.5ml tubes. Both sides of the rack have 12 wells for 1.5ml tubes. Dimensions (L x W x H): 206 x 131 x 54mm. Autoclavable.

Ultra-thin wall design facilitates rapid and steady heat transfer. Smooth interior tube surface minimises binding of enzymes and nucleic acids. Raised rims of wells protect against cross-contamination and provide tight seal particularly in combination with BRANDás new sealing mats.
0.2 ml wells. The 24 / 48 well plates reduce material costs and allow work on a compact PCR plate even with small sample throughputs. Suitable for most types of standard thermal cyclers.

Accessories PCR Mini cooler, PP Alternative PCR Plates 384-well, PP

The use of real-time PCR (qPCR) is becoming increasingly widespread. The line of white PCR products by BRAND offers significantly better results in this application than transparent tubes or plates. The different products in this line are uniformly colored with TiO2 (titanium dioxide), so that in combination with the smooth surfaces, the optimum reflection of the fluorescence signal is provided.
White, PP. Suitable for qPCR. 0.03ml tubes.

Alternative 96 well PCR-Plates, PP, for PCR or qPCR

• Accepts a wide range of microplates and heat seals
• User-friendly control system
• Large digital display shows all operating parameters
• Adjustable sealing temperature: 100°C to 190°C, or no heat
• Precise temperature, timing and pressure for consistent sealing

Specifications
Dimensions (W x D x H):	171mm x 324mm x 362mm
Weight:	12kg
Sealing Temperature:	Off, 100 to 190°C (increments of 1.0°C)
Temperature Accuracy:	±1.0°C
Temperature Uniformity:	±1.0°C
Sealing Time:	0.5 to 10 sec (incements of 0.1 sec)
Compatible Plate Materials:	PP (Polypropylene), PS (Polystyrene), PE (Polyethylene)
Compatible Plate Types:	Standard Assay Plates, Deep-Well Storage plates, PCR Plates (Non-Skirted, Semi-Skirted, Full-Skirted)
Maximum Plate Height:	45mm
Compatible Sealing Film Types:	Foil Polypropylene laminate, Clear Polyester-polypropylene laminate, Clear polymer, Thin clear polymer, Foil-laminate, Foil

White. The sealing paddle helps to attach the self-adhesive film to the plate with a uniform pressure. Especially suited for plates with raised rim.

The Matrix™ SepraSeal sealing mats are suitable for securely sealing sample tubes with or without 2D code. For sample access, the caps can be removed with the practical cap removal tool (Cat.-No. 6.254 390, please order seperately) or pierced once. The pre-slit versions are suitable for multiple piercing. The self-sealing function of the pre-slitted elastomer maintains sample integrity.

• For storage down to -20 °C
• Individual closure or closure of 96 sample tubes simultaneously possible
• Good chemical resistance
• Low evaporation rate

With the Matrix™ DuraSeal sealing mat (Cat.-No. 4.658 312), sample tubes are permanently sealed. The sealing mat is pre-slit for sample access and has a v-shaped design. The special elastomer ensures that the integrity of the sample is maintained. Suitable for sample storage down to -80 °C.

For PP, PS and COC microplates. Transparent, Peelable.

Application:	Quantitative PCR, fluorescence / colorimetric optical assays.
Sealing:	2 to 3 sec 170 to 175°C
Seal Integrity:	-80°C to +110°C, PCR with pressure lids

Clear Polyester backed film, with non peelable seal on Polypropylene microplates.

Application:	Quantitative PCR, optical assays, Roche Lightcycler480, waterbath cycling.
Sealing:	2 to 3 sec 170 to 175°C.
Seal Integrity:	-80°C to 110°C

High-performance plates for manual or automated applications. Suitable for sample storage and preparation, bacteria and yeast cultivation, DNA and RNA isolation, protein analysis and plasmid purification.

• Clear wells
• Frame, white or coloured
• High-contrast, alphanumeric lettering
• Autoclavable (121 °C, 20 min)
• Centrifugation resistance up to 6000 × g
• Rounded RecoverMax^®-Well-Design
• Elevated well frames for reliable sealing, even with heat sealing
• Stackable
• PCR clean: free of human DNA, DNase, RNase and PCR inhibitors or sterile

Alternative

Polyester/Aluminium laminate. Peelable. PP and COC microplates.

Application:	Compound storage. 100% DMSO resistance at room temp. Good on 1536well plates
Sealing:	2 to 3 sec 170 to 175°C
Seal Integrity:	-80°C to +80°C

For PP and PS microplates. Piercable and Peelable. Good solvent / DMSO resistance.

Application:	Compound storage, PCR
Automated workflows:	Piercing and oversealing possible
Sealing:	2 to 3 sec 170 to 175°C
Seal Integrity:	-80°C to +120°C

Cell culture is increasing in popularity in the research and development area. Outside of basic research, cells are cultivated today for a number of reasons, including the production of proteins and in particular as assay systems. As cell cultures can sometimes be quite demanding regarding their environment, the disposables used for cultivation have to be of highest quality. The different cell culture surfaces allow the optimum combination between microplate and specific cell line.

Standard plate for the cultivation of adherent cell cultures. PS-surface with different chemical groups, like e.g. carboxyl and hydroxyl groups, that are freely accessible. Surface is hydrophilic compared with non-treated PS. Incl. one lid per package.

• Sterile
• Manufactured under ISO class 7 cleanroom conditions
• Orange or embossed alphanumeric coding
• Free from endotoxins (< 0.01 EU/ml), DNase, DNA, RNase, non-cytotoxic (according to ISO 10993)
• ANSI/SLAS format

Alternative Microplates for Cell Culture BRANDplates® cellGrade™ plus Alternative Microplates for Cell Culture BRANDplates® cellGrade™ premium Alternative Microplates for Cell Culture BRANDplates® inertGrade™

The thin wall construction optimises the heat transfer from the block to the reaction solution.

Properties:

• Thin wall for optimal heat transfer
• Free of detectable DNase, RNase, human DNA
• Non-Pyrogenic
• For Research Use Only, not for use in diagnostic procedures, unless otherwise indicated

With transparent lid.
For protecting samples prior to warming. The mini PCR cooler keeps samples at 4 °C for approx. 3 hours. The insulating gel changes from violet to pink at 7°C. Suitable for 0.2 ml single vessels, 8-strips, 12-strips as well as 96-well PCR plates.

Ultra-thin wall design facilitates rapid and steady heat transfer. Smooth interior tube surface minimises binding of enzymes and nucleic acids. Raised rims of wells protect against cross-contamination and provide tight seal particularly in combination with BRANDás new sealing mats.
BIO-CERT^® PCR-Quality. The thin walls allow fast transmission of the temperature specified by the cycler, thus reducing cycle times. The stable design of the plates makes them ideal for automation, as they can be gripped by robot systems without twisting. To minimize evaporation with small sample volumes up to 2 µl, the plates can be sealed with appropriate sealing films.

• For automatic high-throughput analyses
• DNase-, RNase-, DNA-, and endotoxin free
• Manufactured under controlled cleanroom conditions

Accessories PCR Mini cooler, PP Alternative 24 / 48 well PCR Plates, non-skirted, PP, for qPCR

BRAND has significantly expanded its product range of extra-thin-wall disposable products, which were specially developed to satisfy the demands of PCR applications.

Quality features

• Suitable for use in common thermocyclers
• PP, extra-thin uniform wall thicknesses to provide the optimal thermal transfer and short cycle times
• 96-well PCR plates with blue alphanumeric code and cut corner marking
• Highly clear flat covers optimally suited for qPCR
• DNase-, DNA- and RNase-free
• Autoclavable at 121°C (2 bar), acc. DIN EN 285
• qPCR: Products which are suitable for Real Time PCR (qPCR)

White PCR products offer significantly better results for qPCR, and are specifically recommended by many thermocycler manufacturers.
The strips of 8 PCR tubes with attached, flat individual caps provide an ideal protection against contamination. The highly transparent cap makes them ideal for a quantitative Real Time PCR. Available in Standard (0.2 ml) or Low Profile (0.15 ml).

Alternative Strips of 8 PCR tubes with attached cap strips

Product features of twin.tec PCR Plates 384:

• U-shaped inner conture of the wells for excellent protection control
• exceptional rigidity and flexure resistance
• continuous skirt for compatibility with automated systems
• semi-skirt for labelling with automated systems
• stackable
• 8 holes in the skirt aid plate positioning and removal from the thermal cycler block e.g. with grippers.
• within SBS footprint recommendations (127.76 ± 0.25 mm x 85.47 ± 0.25 mm)
• suitable for all 384-Well-thermocycler
• 45 µl max. well volume

Designed to fit in a standard 53mm freezer rack, this 144-place PCR Tube Freezer Box will hold individual and/or strip 0.2ml PCR tubes.

• Designed for ULT freezer storage at temperatures down to -86°C
• Durable and impervious to moisture
• Clear polycarbonate lid and base and alpha-numeric index make it easy to identify samples within the box
• Two boxes may be stacked to fit together in one space in a 53mm freezer storage rack if using flat-capped PCR tubes

For PP and PS microplates. Excellently piercable. Not peelable. Very good solvent / DMSO resistance.

Application:	Compound storage, PCR
Automated workflows:	Piercing and oversealing possible
Sealing:	2 to 3 sec 170 to 175°C
Seal Integrity:	-80°C to +120°C.

The new Prime Pro 48 real time PCR system from Techne (UK) is a high specification, economically priced real-time thermocycler. The Prime Pro 48 system has a unique high thermal block uniformity (± 0.1°C). This uniformity as well as validated sample volumes of down to 5µl enable an optimized reaction run time for 40 cycles down to only 15 minutes. The uniform temperature across the plate is achieved with a unique heating and cooling system that quickly enables a uniform temperature of ±0.1°C in every well of the block within a fraction of a second of reaching each well temperature. This high uniformity eliminates the need for triplicates which reduces reagent running costs. Prime Pro 48 accommodates a unique and economical 48-well PCR plate, the size of which is only 1/8 the size of a standard 96-well plate. The well format mirrors a 384-well plate therefore allowing the use of a 16-channel micropipette. The small plate cuts reagent costs in half whilst still producing a strong fluorescent signal. Applications include e.g. absolute and relative quantification, Allelic Discrimination and High Resolution Melt.

Features:

• Validated volume per well: 5 to 20 µl
• High temperature uniformity of ±0.1°C across the whole block instantly after every temperature change
• 40 cycles in 40 minutes before optimization, and down to only 15 minutes when optimized
• Genotype in 4 minutes with over 99% accuracy
• Patented "Adaptive LED Control" minimizes optical artifacts and prevents premature detector saturation
• Dual LED excitation (452nm to 486nm and 542nm to 582nm)
• 4 emission filters (505nm to 545nm, 562nm to 596nm, 604nm to 644nm, 665nm to 705nm)
• Data is always collected in all four filters for all wells
• Distinguishes 5000 and 10000 template copies with 99 percent confidence

Additional features:

• 48-well block
• MIQE compliant
• 400 analyte specific qPCR reagent kits available
• No calibration required
• Calibrated dyes: SYBR^®, FAMTM, HEXTM, ROXTM, Cy^®5.
• Additional dyes within the filter wavelength range are supported with no additional calibration required
• Unlimited software license
• 1 year warranty

Custom 48-well qPCR plates and corresponding seals have to be ordered separately.

Specifications
Temperature range:	30 to 100°C
Temperature stability:	±0.1°C
Temperature uniformity:	±0.1°C within 1sec
Average ramp rate:	5.5°C/sec
Dimensions (W x D x H):	345 x 310 x 320mm
Weight:	13.6kg