Cell culture media and reagents

BME ist der Vorgänger von Eagle Minimum Essential Medium (MEM) und Dulbecco Modified Eagle Medium (D-MEM). Es ist das einfachste der Basismedien mit allen wesentlichen Komponenten für das Zellwachstum. Basalmedium Eagle (BME) begünstigt idealerweise Zelllinien wie HeLa, L-Zellen und primäre Säugetierfibroblasten.

Mc Coy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy’s 5A media support the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.

CMRL-Medien sind besonders für das klonen von Affen Nierenzellen und für das Wachstum vieler anderer Säugetierzelllinien geeignet, wenn sie mit Pferde- oder Kälberserum ergänzt werden.

Glascow Minimum Essential Medium was originally developed by Ian MacPherson and Michael Stoker as a modification of Eagle’s medium (BME). The modifications included adding 10% tryptose phosphate and twice the normal concentration of amino acids and vitamins. This medium was used to study the genetic factors affecting cell competence.

Ham's F10 ist ein beliebtes Medium für das Wachstum von anspruchsvollen Zelllinien.

Ham’s F14 medium was developed from the Ham’s F12, which was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. Ham’s F14 contains a double concentration of amino acids compared to the Ham’s F12. This product is also supplemented with Calcium Chloride and Ascorbic Acid.

The Leibovitz L-15 media were formulated to promote the cell growth in
medium not balanced in CO2. The formulations were developed with the sodium
bicarbonate buffer. The Leibovitz L-15 media are buffered by a complement of salts,
free base amino acids and galactose, so they can be used under conditions of free
gaseous exchange with the atmosphere. When properly supplemented, L-15 Medium supports established cell lines, such as HEp-2 and LLC-MK2, as well as primary explants of embryonic and adult human.

These media were formulated as a reduced serum supplemented medium for the cultivation of human microvascular endothelials cells. For other microvascular cells, it is recommended to be used with growth factors, like EGF or hormones like hydorcortisone.

MEM á is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, thioctic acid, vitamin B12, biotin, and ascorbic acid. MEM á - Modification can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells.