HPLC columns

Base material NUCLEOSIL^® silica, particle size 5µm, pore size 110 Å; polymeric coating - USP L1; eluent in column acetonitrile / water 70:30; allows efficient gradient separation of the 16 PAH in accordance with EPA, detection of the separated PAH by UV (250 to 280nm), with diode array or with fluorescence detection at different wavelegths for excitation and emission (acenaphthylene cannot be analysed with fluorescence detection).
Other HPLC columns on request.
Octadecyl phase PAH, particle size 5µm.
Guard columns for EC columns NUCLEOSIL^® 100-5 C₁₈ PAH, 5µm available on request.

NUCLEOCEL DELTA enantiomer separation based on cellulose derivatives

base material silica,
chiral selector Cellulosetris-(3,5-dimethylphenylcarbamate) - USP L40
similar phases: Chiralcel^® OD, Kromasil^®, CelluCoat™, Eurocel^® 01
standard particle size 10µm

S-Type for high resolution, allows use of shorter columns (150 mm) for faster separations, pressure stability up to ~ 150 bar (2000 psi)

NUCLEOCEL DELTA for normal phase applications:
eluent in column n-heptane - propanol-2 (90:10, v/v)
typical eluents are heptane - propanol mixtures

NUCLEOCEL DELTA-RP for reversed phase applications:
eluent in column acetonitrile - water (40:60), v/v)
designed for use either in polar organic mode or with eluents containing high concentrations of chaotropic salts such as perchlorate

recommended applications: pharmaceutically active compounds, chiral pollutants (e. g. herbicides, PCB), chiral compounds in food (dyes, preservatives), chiral catalysts and bioorganic compounds.
Eluent in column acetonitrile - water, particle size 5µm.

NUCLEOCEL DELTA enantiomer separation based on cellulose derivatives

base material silica,
chiral selector Cellulosetris-(3,5-dimethylphenylcarbamate) - USP L40
similar phases: Chiralcel^® OD, Kromasil^®, CelluCoat™, Eurocel^® 01
standard particle size 10µm

S-Type for high resolution, allows use of shorter columns (150 mm) for faster separations, pressure stability up to ~ 150 bar (2000 psi)

NUCLEOCEL DELTA for normal phase applications:
eluent in column n-heptane - propanol-2 (90:10, v/v)
typical eluents are heptane - propanol mixtures

NUCLEOCEL DELTA-RP for reversed phase applications:
eluent in column acetonitrile - water (40:60), v/v)
designed for use either in polar organic mode or with eluents containing high concentrations of chaotropic salts such as perchlorate

recommended applications: pharmaceutically active compounds, chiral pollutants (e. g. herbicides, PCB), chiral compounds in food (dyes, preservatives), chiral catalysts and bioorganic compounds.
eluent in column n-heptane - propanol-2, particle size 5µm

totally spherical high purity silica - USP L3 pore size 110 Å, pore volume 0,9ml/g, surface (BET) 340m²/g, density 0.47g/ml, pressure stability 800 bar, pH stability 2 - 8; available particle sizes 3µm and 5µm; larger particles (10, 12, 16, 20 , 30 and 50µm) for preparative applications are available as bulk materials.
Particle size 3µm.

pore size 110 Å, particle sizes 3 and 5µm; 2,5%C; not endcapped - USP L8
multi-mode columns (RP and NP)
normal phase chromatography (NP) with hexan, dichloromethane or 2-propanol as mobile phase for polar compounds such as substituted anilines, esters, chlorinated pesticides
reversed phase chromatography (RP) of polar compounds like sugars in aqueous-organic eluent systems
ion exchange chromatography of anions and organic acids using common buffers and organic modifiers
stable against hydrolysis at low pH, working range pH 2-8, 100% stable in water, suitable for LC-MS
Ideal for:
polar compounds under RP conditions (sugars, DNA bases), hydrocarbons under NP conditions
Eluent in column is n-heptane for the NP mode - RP columns are delivered in acetonitrile - water. For changing the solvent system a rinsing step with THF may be necessary.
eluent in column acetonitrile, particle size 3µm.

totally spherical high purity silica - USP L3 pore size 110 Å, pore volume 0,9ml/g, surface (BET) 340m²/g, density 0.47g/ml, pressure stability 800 bar, pH stability 2 - 8; available particle sizes 3µm and 5µm; larger particles (10, 12, 16, 20 , 30 and 50µm) for preparative applications are available as bulk materials.
particle size 5µm.

NUCLEODUR^® C18 - C 8 Gravity nonpolar high density phases

available as octadecyl (C18 -USP L!) and octyl (C8 - USP L7) modifications
Pore size 110 Å; particle sizes 1,8µm, 3µm and 5µm for C18, 1,8 and 5µm for C8
7, 10, 12 and 16µm particles for preparative separations on request
carbon content 18 %C for C18 , 11%C for C8
ideal for method development
allows HPLC at pH extremes (pH 1 - 11)
suitable for LC/MS due to low bleeding characteristics
recommended for overall sophisticated analytical separations
optimal for: pharmaceuticals, e.g. analgesics, antiinflammatory drugs, antidepressants; herbicides; phytopharmaceuticals; immunosppressants
particle size 1.8µm, 18% C

stabel in 100 % aqueous eluent systems- USP L1
pore size 110 Å, particle sizes 1,8µm, 3µm and 5µm; 14 % C
7 and 10µm particles for preparative separations on request
interesting polar selectivity features
excellent base deactivation; suitable for LC/MS due low bleeding characteristics
pH stability 1 - 9
Ideal for: analgesics, penicillin antibiotics, nucleic acid bases, water-soluble vitamins, complexing agetns, organic acids
particle size 1.8µm, 14% C.

NUCLEODUR^® C18 - C 8 Gravity nonpolar high density phases

available as octadecyl (C18 -USP L!) and octyl (C8 - USP L7) modifications
Pore size 110 Å; particle sizes 1,8µm, 3µm and 5µm for C18, 1,8 and 5µm for C8
7, 10, 12 and 16µm particles for preparative separations on request
carbon content 18 %C for C18 , 11%C for C8
ideal for method development
allows HPLC at pH extremes (pH 1 - 11)
suitable for LC/MS due to low bleeding characteristics
recommended for overall sophisticated analytical separations
optimal for: pharmaceuticals, e.g. analgesics, antiinflammatory drugs, antidepressants; herbicides; phytopharmaceuticals; immunosppressants
particle size 1.8µm, 11% C

distinct selectivity based on bifunctional surface coverage - USP L1 and USP L11
pore size 110 Å, particle sizes 1,8µm, 3µm and 5µm; 14 %C
high density of covalently bonded silanes for tailing-free peaks widens the scope for method development
pH stability 1 - 10
suitable for LC/MS due low bleeding characteristics
high reproducibility and consistent quality due to tight QC procedures
range of application: quinolone antibiotics, sulfonamides, xanthines, substituted aromatics
particle size 1.8µm, 14% C