Cell culture media and reagents
Dimethyl sulfoxide, ACS, suitable for cell culture (min. 99.9 %)
BIOSOLUTE®
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RPMI 1640 MEDIUM
RPMI are general purpose enriched media with extensive applications for a broad spectrum of mammalian and hybridoma cells, including human myeloma, mouse hybridoma, human leukocytes, and B and T lymphocytes. It was originally formulated for suspension cultures and monolayer culture of human leukemia cells....
DULBECCO’S MODIFIED EAGLE MEDIUM - DMEM
The DMEM medium is a modification of the BME which contains a higher concentration of amino acids, vitamins and other additives. DMEM is for supporting and maintaining a vast range of mammalian cell types. There are two types of DMEM: one with a high glucose content (4.5g/L), and the other with a low glucose content (1.0g/L). The DMEM low glucose has been developed for the culture of mouse embryonic cells....
MEM W/ EARLE’S SALTS
Minimum Essential Medium (MEM) with Earle’s Balanced Salts is a modification of Eagle’s earlier Basal Medium (BME) which contains a higher concentration of essential nutrients. These media promote the growth of a variety of normal and transformed cells. Since they contain Earle’s Balanced Salts, they are suitable for use in atmospheres charged with CO2 gas....
MEDIUM 199
This complex medium was developed specifically for nutritional research of chicken
fibroblasts. Today, 199 media are widely used for the maintenance of non-transformed cells, vaccine and virus production and primary explants of epithelial cells. The media can be formulated either with Earle salts or Hanks Salts. The media formulated with the Earle salts are buffered with a bicarbonate / carbonic acid solution and retain their pH in a CO2 incubator. The use of the Earle salts under ambient conditions results in a rapid rise in the pH of the culture medium....
Antibiotica
Protect your cell lines from contamination by foreign organisms such as bacteria, fungi and viruses and/or use the antibiotics for selection after transfection.
Most antibiotics suppress the growth of micro-organisms by blocking an anabolic pathway.
Nanomycopulitine actively kills bacteria, including mycoplasma, in all three stages of development without interfering with the eukaryotic metabolism....
ISCOVE’S MODIFIED DULBECCO’S MEDIUM - IMDM
Iscoves media are enriched modifications of DMEM containing sodium selenite. They are excellent for rapidly proliferating high-density cell cultures. The addition of BSA, purified human transferring and soybean lecithin creates a serum free condition ideal for supporting B and T lymphocytes. IMDM was the first media utilizing HEPES buffer. Other cell types can be cultured using this medium under serum free or reduced serum conditions....
Basal medium eagle - BME
BME ist der Vorgänger von Eagle Minimum Essential Medium (MEM) und Dulbecco Modified Eagle Medium (D-MEM). Es ist das einfachste der Basismedien mit allen wesentlichen Komponenten für das Zellwachstum. Basalmedium Eagle (BME) begünstigt idealerweise Zelllinien wie HeLa, L-Zellen und primäre Säugetierfibroblasten....
HAM’S F12 / F-12 NUTRIENT MEDIUM
Ham's F12 was originally developed for serum-free clonal growth of Chinese hamster ovary (CHO) cells, lung cells and mouse L-cells. It is the medium of choice for supporting cells from rodents (especially rabbit and rat) and has proven to be an excellent
cloning medium for the culture of myeloma and hybrid cells (hybridomas)....
GLASGOW MEM BHK 21
Glascow Minimum Essential Medium was originally developed by Ian MacPherson and Michael Stoker as a modification of Eagle’s medium (BME). The modifications included adding 10% tryptose phosphate and twice the normal concentration of amino acids and vitamins. This medium was used to study the genetic factors affecting cell competence....
HAM’S F14 / F-14 NUTRIENT MEDIUM
Ham’s F14 medium was developed from the Ham’s F12, which was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. Ham’s F14 contains a double concentration of amino acids compared to the Ham’s F12. This product is also supplemented with Calcium Chloride and Ascorbic Acid....
LEIBOVITZ L-15 MEDIUM
The Leibovitz L-15 media were formulated to promote the cell growth in
medium not balanced in CO2. The formulations were developed with the sodium
bicarbonate buffer. The Leibovitz L-15 media are buffered by a complement of salts,
free base amino acids and galactose, so they can be used under conditions of free...
MC COY’S 5A MEDIUM
Mc Coy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy’s 5A media support the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines....
SERUM REDUCED MEDIA - MCDB
These media were formulated as a reduced serum supplemented medium for the cultivation of human microvascular endothelials cells. For other microvascular cells, it is recommended to be used with growth factors, like EGF or hormones like hydorcortisone....
MEM ALPHA MODIFICATION
MEM á is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, thioctic acid, vitamin B12, biotin, and ascorbic acid. MEM á - Modification can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells....
Cell Culture Water, Pyrogen free
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Dulbecco's Phosphate Buffered Saline (DPBS) w/o Calcium w/o Magnesium. Sterile
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Dulbecco's Phosphate Buffered Saline (DPBS) 10X w/o Calcium w/o Magnesium
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Dulbecco's Phosphate Buffered Saline (DPBS) w/o Calcium w/o Magnesium, Powder
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Hanks' Balanced Salts Solution (HBSS) w/o Calcium w/o Magnesium w/ Sodium Bicarbonate w/ Phenol Red
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Hanks' Balanced Salts Solution (HBSS) w/ Calcium w/ Magnesium w/ Sodium Bicarbonate w/o Phenol Red
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