The new Prime Pro 48 real time PCR system from Techne (UK) is a high specification, economically priced real-time thermocycler. The Prime Pro 48 system has a unique high thermal block uniformity (± 0.1°C). This uniformity as well as validated sample volumes of down to 5µl enable an optimized reaction run time for 40 cycles down to only 15 minutes. The uniform temperature across the plate is achieved with a unique heating and cooling system that quickly enables a uniform temperature of ±0.1°C in every well of the block within a fraction of a second of reaching each well temperature. This high uniformity eliminates the need for triplicates which reduces reagent running costs. Prime Pro 48 accommodates a unique and economical 48-well PCR plate, the size of which is only 1/8 the size of a standard 96-well plate. The well format mirrors a 384-well plate therefore allowing the use of a 16-channel micropipette. The small plate cuts reagent costs in half whilst still producing a strong fluorescent signal. Applications include e.g. absolute and relative quantification, Allelic Discrimination and High Resolution Melt.

Features:

• Validated volume per well: 5 to 20 µl
• High temperature uniformity of ±0.1°C across the whole block instantly after every temperature change
• 40 cycles in 40 minutes before optimization, and down to only 15 minutes when optimized
• Genotype in 4 minutes with over 99% accuracy
• Patented "Adaptive LED Control" minimizes optical artifacts and prevents premature detector saturation
• Dual LED excitation (452nm to 486nm and 542nm to 582nm)
• 4 emission filters (505nm to 545nm, 562nm to 596nm, 604nm to 644nm, 665nm to 705nm)
• Data is always collected in all four filters for all wells
• Distinguishes 5000 and 10000 template copies with 99 percent confidence

Additional features:

• 48-well block
• MIQE compliant
• 400 analyte specific qPCR reagent kits available
• No calibration required
• Calibrated dyes: SYBR^®, FAMTM, HEXTM, ROXTM, Cy^®5.
• Additional dyes within the filter wavelength range are supported with no additional calibration required
• Unlimited software license
• 1 year warranty

Custom 48-well qPCR plates and corresponding seals have to be ordered separately.

Specifications
Temperature range:	30 to 100°C
Temperature stability:	±0.1°C
Temperature uniformity:	±0.1°C within 1sec
Average ramp rate:	5.5°C/sec
Dimensions (W x D x H):	345 x 310 x 320mm
Weight:	13.6kg

Alternative Real-time PCR-system Eco 48

Mid-size thermocyclers - find space on any laboratory bench

• Approved cycler with new design
• With and without gradient function
• Gradient upgrade from ³PRIMEX to ³PRIMEG
• 3.5" colour touch screen with graphical display
• Height-adjustable and heatable lid, temperature from 100°C to 115°C, with ON/OFF switch and automatic switch off against overheating
• Password protected
• Auto-Restart
• USB port
• 4 years warranty for the Prime Thermocycler

Specifications
Block temperature:	+4°C to +100°C
Temperature gradient (only 3PRIMEG or 3PRIMEX with upgrade):	+30°C to +80°C
Max. Gradient:	+14°C
Block uniformity at 55°C:	<±0.3°C
Max. Heating rate:	3.0°C/s
Dimensions (W x D x H):	210 x 350 x 180

Alternative Thermocycler Alpha Cycler

High specification, economically priced real-time thermocycler. The Eco 48 system has a unique high thermal block uniformity (± 0.1°C). This uniformity as well as validated sample volumes of down to 5 µl enable an optimized reaction run time for 40 cycles down to only 15 minutes. The uniform temperature across the plate is achieved with a unique heating and cooling system that quickly enables a uniform temperature of ± 0.1°C in every well of the block within a fraction of a second of reaching each well temperature. This high uniformity eliminates the need for triplicates which reduces reagent running costs. Eco 48 accommodates a unique and economical 48-well PCR plate, the size of which is only 1/8 the size of a standard 96-well plate. The well format mirrors a 384-well plate therefore allowing the use of a 16-channel micropipette. The small plate cuts reagent costs in half whilst still producing a strong fluorescent signal. Applications include e.g. absolute and relative quantification, Allelic Discrimination and High Resolution Melt.

Features:

• Validated volume per well: 5 to 20 µl
• High temperature uniformity of ± 0.1°C across the whole block instantly after every temperature change
• 40 cycles in 40 minutes before optimization, and down to only 15 minutes when optimized
• Genotype in 4 minutes with over 99% accuracy
• Patented "Adaptive LED Control" minimizes optical artifacts and prevents premature detector saturation
• Dual LED excitation (452 nm to 486 nm and 542 nm to 582 nm)
• 4 emission filters (505 nm to 545 nm, 562 nm to 596 nm, 604 nm to 644 nm, 665 nm to 705 nm)
• Data is always collected in all four filters for all wells
• Distinguishes 5000 and 10000 template copies with 99 percent confidence

Additional features:

• 48-well block
• MIQE compliant
• 400 analyte specific qPCR reagent kits available
• No calibration required
• Calibrated dyes: SYBR^®, FAMTM, HEXTM, ROXTM, Cy^®5.
• Additional dyes within the filter wavelength range are supported with no additional calibration required
• Unlimited software licence
• 2 years warranty

Scope of supply:
Real-time PCR-system Eco 48, EcoControl and EcoStudy software, USB stick, 50 plates, 50 plate seals, power and Ethernet cable

Specifications
Temperature range:	30 to 100°C
Temperature stability:	±0.1°C
Temperature uniformity:	±0.1°C within 1sec
Average ramp rate:	5.5°C/sec
Dimensions (W x D x H):	345 x 310 x 320mm
Weight:	13.6kg