Ready-to-use colloidal Coomassie® solution that allows protein staining within 15 minutes. Contains no organic solvents and no phosphoric acid. Very high sensitivity (> 5 ng /band) with low background for good quantification and high band sharpness. Suitable for native page and SDS page, MS compatible.

Features & Benefits:

• Staining in only 15 minutes
• Very high sensitivity (> 5 ng /band)
• No use of hazardous chemicals
• Re-usable up to 3 times
• Shelf life: 1 year at room temperature

Manufactured by a proprietary heat-shock fractionation process, using caprylic acid as an albumin stabilizer. Standard quality for many applications: protein standard, growth promoter in serum-free media for the cultivation of animal cells, supplement in microbiological nutrient media, diluent/stabilizer in diagnostic systems and of isolated enzymes, peptides or antibodies as well as blocking agent to prevent non-specific absorption in immunoassays like Western Blots, ELISA systems.

Assay based on the bichinchoninic acid method. Proteins reduce alkaline Cu(II) to Cu(I). Bichinchoninic acid forms a purple complex with Cu(I) with an absorbance maximum at 562 nm. The absorbance is directly proportional to protein concentration.

• Fast and sensitive assay: linear detection range from 25 - 1000 µg protein/ml
• Easy to use: contains ready-to-use reagents and protein standard
• Compatible with many detergents
• Less binding variation between different proteins than Bradford assay

The assay bases on the bichinchoninic acid method (1). Proteins reduce alkaline Cu(II) to Cu(I). Bichinchoninic acid forms a purple complex with Cu(I) with an absorbance maximum at 562 nm. The absorbance is directly proportional to protein concentration.

• Fast and sensitive assay: linear detection range from 0.5 - 20 µg protein/ml
• Easy to use: contains ready-to-use reagents and protein standard
• Compatible with many detergents
• Less binding variation between different proteins than Bradford assay

Protein-free, polymer-based blocking reagent for fast blocking without loss of sensitivity. With BlueBlock, the specific binding sites remain accessible while nonspecific reactions are suppressed, thus leading to an increase in signal intensity.

• Short incubation times and increased signal intensity
• Complete procedure in only 1 - 2 h with All-in-One protocol
• For colorimetric, chemiluminescence and fluorescence detection systems

For fast and gentle electrotransfer of proteins in Western Blots. The BlueBlot semi-dry blotters form a homogeneous electrical field that guarantees fast and efficient transfer of proteins from gel to membrane. As associated with semi-dry blotting compared to tank blotting less heat is generated for gentle protein transfer. It is fast and requires less buffer. Moreover, all common continuous and discontinuous buffer systems can be applied without any limitations. The electrodes are built into a stable acrylic housing that is resistant to 10 % ethanol and easy to clean. The long-lasting electrodes can be dismounted and cleaned separately.

Features & Benefits:

• Platinum-covered steel net as anode, stainless steel cathode
• Spring-mounted anode allows blotting of thicker gels and gel stacks
• Blotting areas SD11: 11 cm x 11 cm, SD17: 17 cm x 17 cm, SD26: 24 cm x 26 cm
• SD11 expandable to 17 cm x 17 cm with 11835802, SD17 usable for minigels 11 cm x 11 cm with11835801
• Weight: 3 kg / 4 kg (SD26)
• Made in Germany

The BlueVertical™ PRiME™ is a dual minitank system for one or two pre-cast gels and is perfectly suited for protein separations by SDS-PAGE, native PAGE, IEF as well as nucleic acid separations by denaturing or native PAGE.
The instrument consists of an outer buffer tank made of robust, transparent acrylic and the inner running unit. A safety lid closes the top and results in a very compact and robust design.
The outer buffer tank works as a heat sink (passive buffer cooling) and is sufficient for most applications mentioned above.

• Unique clamp system, complete leak-free
• Installation of the gels in seconds
• Made in Germany

Fluorobind PVDF membranes show an excellent protein binding capacity. Suitable for all standard applications in protein analysis, and as well for special applications as fluorescence detection and protein sequencing.

• Ideal for blotting of proteins of lower molecular weight and of peptides
• As well suitable for larger proteins
• High sensitivity with low background in all common detection systems
• High mechanical stability facilitates handling
• Allows multiple stripping of the membrane and harsh washing conditions

For the staining of blotted proteins on nitrocellulose, PVDF membranes and cellulose acetate membranes The reversible staining is ideally suited for subsequent immunoblots (Western blots) or other staining procedures, as Ponceau S can be completely removed.

Features & Benefits:

• Protein staining on membranes
• Completely reversible
• In 3 % Trichloroacetic acid

SERVA Fluo-610 Standard I is a ready-to-use fluorescent labelled protein marker for direct detection in a SDS-PAGE gel or on a membrane in Western Blotting. The marker proteins in the range from 14.4 kDa to 9.4 kDa are labelled with the highly sensitive and stable fluorescent dye Lightning Red. SERVA Lightning Red (cat. no. 43402) is a fluorescent dye for rapid labelling in minutes of proteins prior to SDS PAGE and/or Western Blotting, making any staining and washing steps after electrophoresis unnecessary.