Microbiology (Cultivation)

The LABSOLUTE® baffled flasks are made of high-quality borosilicate glass 3.3 and are therefore especially temperature and chemical resistant. The four baffles on the bottom cause a turbulent flow increasing the gas exchange surface and producing a higher oxygen transfer. The autoclavable flasks can be used with all common laboratory shakers.

Erlenmeyer shape. Including PP screw cap.

The LABSOLUTE® baffled flasks are made of high-quality borosilicate glass 3.3 and are therefore especially temperature and chemical resistant. The four baffles on the bottom cause a turbulent flow increasing the gas exchange surface and producing a higher oxygen transfer. The autoclavable flasks can be used with all common laboratory shakers.

Erlenmeyer shape.

The LABSOLUTE® disposable inoculation loops have a flexible loop at one end to inoculate soft agar culture media and a needle for picking individual colonies at the other end. The disposable inoculation loops are packed sterile in a paper/plastic blister of 20 pieces.

• Length: 195 mm

The LABSOLUTE® petrifilm rack is suitable for the storage of 3M petrifilms. Using the rack the films can be stored space-saving and economic in every common incubator. The rack is made of white powder-coated metal wire.

• Capacity for up to 100 petrifilms
• Heat-resistant
• Dimensions (W x H x D) 82 x 97 x 93 mm

The LABSOLUTE® petri dishes are made of crystal-clear PS and offer high optical clarity. The dishes with three vents support gas exchange and are ideal for short incubation times. Dishes without vents are best suited for anaerobic and long-term work. The Petri dishes are prepared and packed under aseptic conditions. Sterilisation for the sterile dishes is done by beta rays.

• Perfectly stackable
• Heat-resistant from -40 - 70 °C

The L- and T-shaped spatulas from LABSOLUTE® are ideally suited for the uniform plating of large volumes.

• Packed individually or 5 pieces each
• Smooth, rounded surface
• Gamma sterile

The LABSOLUTE® rack for petri dishes is suitable for 30 dishes up to a diameter of 90 mm and designed to be used on the bench or mounted on the wall.

• Dimensions (W x H x D): 107 x 236 x 222 mm
• Fastening material included

Made of flexible polystyrene. With ultra-smooth loops for problem-free planting and streaking of cultures. Free of lubricants, oils and electrostatic charges, enabling consistent and complete liquid transfer. Ergonomic design of the loops for optimal grip and an easy orientation of the loop head. Needle end shape is perfect for picking off individual isolated colonies and suitable for making stab inoculations into agar slants or tubes of solid culture medium.
Package: 10 bags of 10 pieces

For easy plating.

Cellulose, 100 % biodegradable and fully compostable autoclavable. Steristoppers^® have been proved as an excellent sealing for microbiological samples and tissue cultures in test tubes and Erlenmeyer flasks. They are in constant use in many European microbiological laboratories.

• Autoclavable
• Filters bacteria
• Sterilizable
• Air permeable

Media for bacteria
Luria Bertani (Miller):

• Standard high salt content media for E. coli cultivation
• Ideal for plasmid preparation

• Standard medium with low salt content for the cultivation of E. coli
• Ideal for cultivating recombinant E.coli strains

• Rich content media for the high density cultivation of E. coli
• Ideal for protein expression and plasmid preparation

• Nutritive medium for the cultivation of recombinant E. coli strains and for the growth of filamentous bacteriophages

• High quality agar used for the preparation of culture media and other bacteriological applications

• Standard Agar based on Luria Bertani (Miller) media with high salt content
• Ideal for growth and maintaining especially of E. coli strains used in molecular microbiology procedures

• Standard Agar based on Luria Bertani (Lennox) media with low salt content
• Ideal for growth and maintaining especially of recombinant E. coli strains

• Agar for the cultivation of Aliclyciclobacillus spp. in fruit juices

• Agar for isolation, cultivation and enumeration of acid-tolerant microorganisms

• Non-selective agar for the cultivation and standardized enumeration of aerobic microorganisms

• Standard medium for the cultivation of Saccharomyces cerevisiae and other yeasts

• Concentrate of the water-soluble portion of autolyzed Saccharomyces cerevisiae cells
• Non-animal product, used widely for non-animal formulations of bacterial, fungal, mammalian and insect cell culture

• Pancreatic digest, tryptone from casein
• Excellent nutrient for use in culture media for producing antibiotics, toxins, enzymes and other biological products

• Pancreatic digest, peptone from casein
• Ideal for use in fermentation applications and microbiological culture media

Ideal for the uniform spreading of cell cultures and liquids.

• Smooth , rounded surfaces for uniform spreading without scratching the agar surface
• Ethylene oxide sterilized
• Sterile, 10 per bag or individually packed

Radiation-sterilised. Semi-quantitative standard for sample handling, e.g. for diseases of the urinary tract. Surface treated to increase drop adhesion. Reduced contamination risks as product is disposable and does not require flaming.

Platinum-iridium 90/10 wire available in different diameters.
Sold per cm.

DURAN^®. Erlenmeyer. Straight neck. Suitable for metal caps. Glass Type I / neutral glass as per USP, EP and JP. With retrace code (Batch Identification). Certificate available via the Internet. Autoclavable.

Clear glass. DIN version. Autoclavable.

Constructed in steel wire, as outlined below. Suitable for dish diameters of 100mm, Internal diameter approx. 102mm, Internal height approx. 230mm. For approx. 10 petri dishes.

DIN thread. Complete with red PBT screw cap with PTFE-faced sealing wad. Thus, it is suitable for the cultivation of microorganisms. Glass Type I / neutral glass as per USP, EP and JP. Autoclavable.

Ideal for sampling in laboratories

9.413 160: non-sterile, according to EN ISO 13485
9.413 161: sterile, according to EN ISO 13485 and conforming to 93/42/EEC, enclosure V for medical products, sterilised with ETO gas

For culture flasks and culture medium bottles.

Single or double cotton bud. Wooden, PP or paper applicator.
WA 1-l, WA 1 D: Calibrated wooden rod with minimum diameter tolerances.
WA 1s, WA 1s PL, WA 1s 2x, WA 1s 25x, WA 2 PLs: Single, double or 25 pieces packed in peel-pack, EO-sterilized.
WA 7, WA 7 PL: Double cotton bud.
WA P: Flexible, bendable

From Soda-lime glass. With DIN thread and PP screw cap

With GL 45 thread

• Oxygen introduction is often a limiting factor for cell growth.
• Baffled flasks cause a turbulent flow, increase the gas exchange surface and produce a higher oxygen transfer.
• Automated one-step production enables a reproducible and comparable result.
• Complete with membrane screw cap (gas exchange).

• Ideal for culture work
• Manufactured from Pyrex^® borosilicate glass
• High resistance to attack from water reduces leaching of contaminants which can cause pH

• Phenolic screwcaps with inert PTFE lined rubber disc
• Cap can withstand repeated dry heat and steam sterilisation cycles (121°C for 2 hours)

Available in three lengths and two needle sizes to suit a wide range of application needs. The Mini fits neatly into a 1.5 mL microfuge tube allowing the lid to be closed for full sample transfer/recovery.

• Naturally biodegradable White Birch wood
• Manufactured in an FDA-registered factory and complies with FDA standards for food contact
• Autoclavable and available in semi or needle point styles for delicate operations

Sterile, dehydrated culture media pads in petri dishes. Once they are moistened with 3.0 ml to 3.5 ml of sterile and demineralised (or distilled) water they are ready-to-use immediately. All Nutrient Pad sets are supplied with the appropriate membrane filters, which are also individually sterile packed. Shelf life of the NPS 18 to 24 months at room temperature. Alternative nutrient media are available on request.

• Ready to use
• 90 % less material volume
• Validated methods
• Time and energy saving
• Increased productivity and reliability
• Low operating costs
• Fast and reliable evaluation

Inoculation loops made from stainless steel, special stainless steel wire or platinum-iridium wire (90/10). Can be heated to red heat.
Length: 60mm. Wire diameter: 0.5mm.

Inoculation loop holder according to Kolle for 0.3 to 0.7mm wire diameter. Stainless steel with handle. Fixed by union nut.

Inoculation loop stand robust and sturdy made of plastic (autoclavable). Accomodate up to 6 inoculation loop holders at any one time.

SILICOSEN^® and BIO-SILICO^® are specially processed culture plugs in sterile rubber with continuous bubbles for preparation, filling and sterilisation of culture media. Their uniform pore structure and good air permeability make them excellent for the cultivation of aerobic microorganisms.

The choice between SILICOSEN^® and BIO-SILICO^® culture stoppers depends on the requirements of the application. SILICOSEN^® culture stoppers have low water evaporation which makes them particularly suitable for long term cultures. BIO-SILICO^® culture stoppers have high air permeability, similar to cotton plugs. Both types are chemically resistant, temperature resistant, liquid repellent and reusable.
Superb permeability and fits tight onto the media container due to sealing lip, where importance is placed on mounting quality. Suitable for shaking cultures.

Erlenmeyer. With 3 side baffles. Neck straight or wiht beaded rim.

SILICOSEN^® and BIO-SILICO^® are specially processed culture plugs in sterile rubber with continuous bubbles for preparation, filling and sterilisation of culture media. Their uniform pore structure and good air permeability make them excellent for the cultivation of aerobic microorganisms.

The choice between SILICOSEN^® and BIO-SILICO^® culture stoppers depends on the requirements of the application. SILICOSEN^® culture stoppers have low water evaporation which makes them particularly suitable for long term cultures. BIO-SILICO^® culture stoppers have high air permeability, similar to cotton plugs. Both types are chemically resistant, temperature resistant, liquid repellent and reusable.
Moisture evaporation is minimal, thus there is less drying of the culture medium. Colour: natural.

Rimless. For use with Kapsenberg caps. Glass Type I / neutral glass as per USP, EP and JP. Autoclavable.

Beaded rim. Glass Type I / neutral glass as per USP, EP and JP. Autoclavable.

Anodised in a choice of colours.
Special caps for sterile, but not hermetically sealing, closure of culture flasks and media bottles with straight, rimmed or rimless necks as indicated. With nichrome steel springs that clamp onto the outer wall of the container.
Cleaning advice available on request.

For test tubes, culture tubes and culture bottles.

• Aluminium screw cap (autoclavable / sterilisable)
• Plastic screw cap (steam-autoclavable with 121 °C / sterilisable)
• Complete with rubber gasket

Rimless. For glass caps. Glass Type I / neutral glass as per USP, EP and JP. Autoclavable.

Cell culture multiwell plates are available in the hydrophilic surface (TC surface treatment) for improved cell adhesion and hydrophobic surface for suspension cultures and hybridoma cells. All panels can be stacked together, both with and without cover. The special design of the panels is also in stacked plates a faster heat exchange over the entire bottom surface of each plate allows . All plates have an imprinted lot number and are both pyrogens and DNase / RNase - free .

Properties:

• High clarity and low autofluorescence
• Lid enables optimal gas exchange with the lowest possible evaporation
• Single position lids to prevent cross-contamination
• Alphanumeric well coding
• Compatible with common instruments and automated systems
• Easy-to-open packaging

SILICOSEN^® and BIO-SILICO^® are specially processed culture plugs in sterile rubber with continuous bubbles for preparation, filling and sterilisation of culture media. Their uniform pore structure and good air permeability make them excellent for the cultivation of aerobic microorganisms.

The choice between SILICOSEN^® and BIO-SILICO^® culture stoppers depends on the requirements of the application. SILICOSEN^® culture stoppers have low water evaporation which makes them particularly suitable for long term cultures. BIO-SILICO^® culture stoppers have high air permeability, similar to cotton plugs. Both types are chemically resistant, temperature resistant, liquid repellent and reusable.
Cell size is uniform, which provides stable and good permeability. Optimum product for shaking cultures-durability and operability are excellent.

SILICOSEN^® and BIO-SILICO^® are specially processed culture plugs in sterile rubber with continuous bubbles for preparation, filling and sterilisation of culture media. Their uniform pore structure and good air permeability make them excellent for the cultivation of aerobic microorganisms.

The choice between SILICOSEN^® and BIO-SILICO^® culture stoppers depends on the requirements of the application. SILICOSEN^® culture stoppers have low water evaporation which makes them particularly suitable for long term cultures. BIO-SILICO^® culture stoppers have high air permeability, similar to cotton plugs. Both types are chemically resistant, temperature resistant, liquid repellent and reusable.
Superb permeability. Suitable for aerobic bacterium cultures and shaking cultures.

Rimless. Glass Type I / neutral glass as per USP, EP and JP. Autoclavable.

SILICOSEN^® and BIO-SILICO^® are specially processed culture plugs in sterile rubber with continuous bubbles for preparation, filling and sterilisation of culture media. Their uniform pore structure and good air permeability make them excellent for the cultivation of aerobic microorganisms.

The choice between SILICOSEN^® and BIO-SILICO^® culture stoppers depends on the requirements of the application. SILICOSEN^® culture stoppers have low water evaporation which makes them particularly suitable for long term cultures. BIO-SILICO^® culture stoppers have high air permeability, similar to cotton plugs. Both types are chemically resistant, temperature resistant, liquid repellent and reusable.
Moisture evaporation is minimal, thus there is less drying of the culture medium. Colour: natural.

Durham test tubes are mainly used in microbiology to detect gas produced by micro organisms. These special tubes are placed upside down in bigger test tubes.

• Made of soda lime glass
• With straight rim and with round bottom
• Ungraduated

Wironit. CrNi 18/12.

Aluminium. For the closure of culture tubes, bottles and flasks.

Steriplan^®. Hydrolytical class 3.

• Without graduation
• PP screw cap, with white TPE elastomer seal
• Autoclavable at 121 °C (2 bar), according to DIN EN 285

Glass Type I / neutral glass as per USP, EP and JP. Autoclavable.

Manually operated turntable guarantees smooth and easy rotation in both directions. Also enables easy and fast allocation of bacteria across petri dish surface. Double-sided construction accomodates petri dishes from 60 to 150 mm diameter by inverting the turntable. Ball bearings provide smooth and effortless spinning, ensuring rapid inoculation steps and extended operation life. Light weight but robust body manufactured from aluminum with non-skid footprint. The silicone pad holds the petri dishes firmly and always keeps the dish centered during inoculation steps. The non-skid pad can easily be removed for fast and efficient cleaning.

Perfect tool to protect petri dish interior contents from dust, moisture and other environmental effects. Waterproof. Chemically inert and resistant to most organic solvents and caustic agents. Remains flexible in moderate freezing temperatures. Can resist up to 100 °C. Stretches and widens-up 50 % of original length. Clings around the petri dish body and seals irregular perimeters of petri dishes with leak-proof tightness. Also can be used to seal bottle necks and caps.

DURAN^®. Roux type. With fire-polished, offset neck. Glass Type I / neutral glass as per USP, EP and JP. Autoclavable.

Transparent. With baffles indented into the base which increases mixing when trypsinizing and aerating in processes such as cell culture and fermentation. Autoclavable. 500 ml and 1000 ml flasks have a straight neck, suitable for 38 mm standard caps of stainless steel, aluminium or plastic.

Anodised in a choice of colours. For sterile, but not hermetically sealing, closure of containers. With nichrome steel springs that clamp onto the outer wall of the container. Autoclavable.
Cleaning advice available on request.

The STERI second sterilisers permit instant disinfection and sterilization of all small instruments directly at the workplace. Especially suitable for laminar flow cabinets. Biological investigations and tests prove that the 250 °C temperature maintained in the glass bead container guarantees complete destruction of all micro-organisms and spores within a few seconds. The one part of the instrument which is inserted into the STERI glass beads is sterilized. The STERI 250 and 350 are two versions of the same quality apparatus, with a stainless steel container, high-performance heating element, reduced heating time, high precision thermostat, temperature limiter and built-in thermometer.

• For perfect hygiene directly at the workplace
• A guaranteed Swiss quality product

For the cultivation of anaerobic and microaerophilic microorganisms in a defined and rapidly generated gas atmosphere.The requested atmosphere may be reached under ideal conditions by two methods. Either by using chemical gas packs (anaerobe systems) or by manually evacuating the jars with a vacuum pump and flushing with gas afterwards (e.g. with nitrogen), in this case no chemical accessories are needed (except anaerobic jar eco and crystal eco). The jars are made of robust stainless steel or transparent PC.The lids are made of UV-resistant plastic or transparent polycarbonate with two corner valves incl. tube clips for vacuum hoses (5 mm i.d.) and with manometer for exact control of the vacuum or overpressure from -1 to 0.2 bar (except anaerobic jar eco and crystal eco). The optional racks are made of stainless steel providing holders for comfortable operation of the anaerobe systems.

Methods for reaching anaerobic conditions: 3 x times evacuating and filling gas, flushing with gas for 5 minutes and chemical gas production (GasPacks) for Anaerobic jars "small", "standard", "large" and "crystal". For Anaerobic jar "eco" flushing with gas for 5 minutes (Using GasPack-Kits).

Customized: Other equipment available upon request. Examples: customized lids, e.g. made of special or heat-resistant materials e.g. stainless steel, heat-resistant pressure gauges (up to 100 °C), also without cocks / manometer available.

For microbiological quality control. Quick connectors at both sides of the manifolds enable fast connections of either tubing, another manifold or end caps. Various funnel and filtration units are available.

• Full autoclavability without disassembly
• Sterile venting
• Reduced risk of secondary contamination
• Low working height
• Rubber feets providing better stability at the workplace

Cell culture multiwell plates are available in the hydrophilic surface (TC surface treatment) for improved cell adhesion and hydrophobic surface for suspension cultures and hybridoma cells. Cell culture microplates from the CELLSTAR® range are specifically treated. This treatment leads to polar groups such as carboxyl and hydroxyl groups , added to the plastic surface. These allow the growth of adherent cell lines. They are also suitable for automated systems and contain an alphanumeric well coding. They are also suitable for footprint systems and contain an alphanumeric well coding.

Properties:

• High clarity enables optimal microscopic examinations
• Lid enables optimal gas exchange with the lowest possible evaporation
• Stackable
• Alphanumeric well coding
• Individually wrapped peel-off bags
• Consecutive lot numbering
• 384 and 1536 well foramt available

This agaroses are suitable for routine analysis of nucleic acids. They combine tensile strength with low electroendoosmotic effects to achieve high resolving power for a wide size range of nucleic acid fragments. AG agaroses are also very environmentally friendly, being manufactured by a process which excludes organic solvents. Also available as low melting point agarose for nucleic acid recovery and enzymatic applications (Cat. No. 4.658 455) and as High Resolution PCR grade agarose for separation of small DNA fragments of up to 800 bp (Cat. No. 6.270 615). Other packing sizes on request.

Matt finish. Without internal clips. Loose-fit caps for sterile, but not hermetically sealing, closure of culture flasks and other containers. Can also be used as open specimen holders. Autoclavable.

Accelerated bacterial enumeration. Fast spiral plating of Petri dishes replacing up to 4 dilutions / platings. A set volume of the sample is spread in a spiral pattern with logarithmically decreasing volume on the surface of the petri dish : the dish is divided into defined sectors where the volume given is precisely known. Sensitivity from 30 to 10⁷ CFU/ml.
100% new platform with revolutionary rotating arm design allows simplified mechanics and hight speed movements: full cycle in 25 seconds.
100% new disinfection system:

• The stylus is cleaned inside and outside by over flow.
• The cleaning liquid bottles have sterile connectors, and are fully autoclavable .
• More than 600 platings can be made without changing the cleaning reservoir)
• Security detectors for lack of cleaning liquid

• Manufactured from Pyrex^® borosilicate glass
• Exhibits 4 baffle indents to provide optimal and consistant agitation

For cultivating plant material or for cell cultures. Rolled rim dishes with slightly tapering sides and drop-on cover. Stable, even when empty and stackable to save space. An accessory clamp can be used to fasten the lids down tightly.

• Manufactured from Pyrex^® borosilicate glass
• Erlenmeyer
• Straight neck
• Suitable for metal caps

To DIN 38411. Rimless. Suitable for use with Kapsenberg caps. Glass Type I / neutral glass as per USP, EP and JP. Autoclavable. Please order cap separately.

From Soda-lime-glass. Straight rim.

Needles ideal for picking single colonies. Flexible loops for easier collection and inoculation.

Proporties:

• Sterilised by irradiation
• Colour coding for volume differentiation
• Single and bulk packaging options

For use in preparation of cultures.

• Large surface area to volume ratio
• Three laterally integrated baffles, to provide optimal and consistant agitation
• Available in 2 versions: with Delong neck, for use with culture flask caps or with PP screw cap, GL45

Platinum-iridium wire 90/10.
0.5mm thick.

For uniform inoculation of petri dishes. Easy-to-use. Made in Germany.

schütt petriturn-M
With manually operated, heavy turntable for smooth and long-lasting rotation. Double-sided construction accommodates two sizes of petri dish (90mm or 150mm diameter), by inverting the turntable.

schütt petriturn-E
Electrically driven (100 - 240 V, 50/60 Hz), constant speed rotation. Easy operation of the rotary table contact-free start via IR sensor (adjustable rotation time from 3 to 120 sec.), in continuous operation or optionally in foot switch mode. Infinitely adjustable speed from 10 to 120 rpm. For Petri dishes up to Ø 100 mm ( Ø 150 mm optional). Compact housing made of stainless steel.