Cell disruption

Polished stainless steel, micropestles. Precisely fitted for Eppendorf Safe-Lock Tubes™, manufactured in one-piece, smooth handle sections, can be disinfected easily. Minimum homogenate displacement due to tapered piston.

For Reaction tubes:
For homogenisation of cells, tissues or foodstuffs / feed samples.

• For tubes with 0.5 ml, 1.5 ml, and 2.0 ml
• Can be cooled down to -196 °C
• Sterilisable in an autoclave or dry heat at 200 °C
• Suitable for PCR applications
• Alternative to single-use plastic pestles
• Perfectly suitable for Eppendorf Safe-Lock Tubes

96 Well Polystyrene Microplates are manufactured under DIN ISO 9001 guidelines and can be traced all the way back to production through a defined LOT number system. Furthermore they are footprint compatible with automated systems. Polystyrene is a highly clear polymer with excellent optical properties which makes it ideal for precise optical measurements. An it is suitable for work with cell cultures.

Properties:

• Are manufactured without the use of siliconbased mould release
• U-Bottom: ideally suited for agglutination tests, no sharp corners
• V-Bottom: entire sample volume must be pipetted off
• F-Bottom / Standard (ST): for precise optical measurements, microscopic applications (bottom reading)
• Are analysed for detectable DNase, RNase and human DNA
• Are free of biocides and antistatics

For safety grinding of any cells by minimal temperature rising. Closed System (CS) for minimizing the risk of personal contact with aerosols and sample overflow while grinding. Optimal contact of grinder and conical sample tube (15 or 50ml) to grind each cell in the best way.
Each unit is gamma sterilized and comes with a fully assembled pestle , conical sample tube (PP), solid top cap (HDPE) and label.
Type CS1: Tip from glass-filled PP (30%/70%) for standard grinding
Type CS2: Vitrified tip (cast silica) for harder grinding

For resuspending pellets and homogenising within tubes (1.5, 2.0ml, exact fit). Autoclavable. Suitable for homogeniser/motor drive.

Accessories PCR tubes strips, with attached individual caps, PP Accessories Micro pestles, accessory drive motor Alternative Homogenisers with PTFE or glass pestles

Autoclavable polypropylene. Suitable for battery-operated drive motor (9.314 496). Pestle length 7cm. Supplied with reaction tube, except Cat. No. 9.314 492 and 9.314 493.

Accessories Micro pestles, accessory drive motor Alternative  Alternative Micro pestles, CTFE / stainless steel

Glass. Suitable if the nuclei are to be maintained after homogenising.
Two pestles are supplied:

• Pestle "A" for pre-grinding
• Pestle "B" for fine grinding

Spare components are available and are completely interchangeable.

Accessories Spare components for dounce homogenizers Alternative Homogenisers DUALL®, with glass pestle Alternative Homogenisers DUALL®, with PTFE pestle

• Precision Bore Borosilicate glass mortars with a range from 5.0 to 55ml capacity
• Borosilicate glass pestle
• All parts are fully autoclavable

Grinding Clearance Pestle and Mortar: 0.152 to 0.254mm

Potter Elvehjem & Dounce. Safe homogenisation without much effort, for gentle digestion of biological samples and tough, difficult to homogenize material, so that a high proportion of intact cell nuclei can be isolated.

• Large stability, vibration-free due to robust 3-fold stand construction
• 4-digit display for reproducible homogenisation
• Easy handling
• Steplessly adjustable, electronic speed control from 40 - 3000 min-1
• High torque drive, powerful even at low speeds
• Flexible use
• Large selection of conical and cylindrical homogenizers and pestle (PTFE, glass and also glass fibre reinforced)
• Tubes with volumes from 1.5 to 40 ml and outer diameters from 10 to 40 mm can be used
• Use of Eppendorf^® microreaction vessels with 1.5 ml possible
• Chuck with hollow shaft for quick and easy pestle change and the use of different shaft lengths and diameters
• For optimum cooling, the cooling vessel can be filled with ice or ice water

Supplied with: schuett homgen^plus homogeniser drive unit with universal keyless chuck, incl. cooling vessel, 1000 ml and universal chuck set (consisting of 6 sizes).

We also offer a sterile alternative for use in a glove box and with infectious material. Details on request.

With conical and cylindrical area for effective tissue for grinding and also for homogenising. Especially suitable for cellular tissue such as muscle, heart and lungs. The pestle is sufficiently stable to be motor driven. The space between the pestle and mortar is between 0.1mm to 0.15mm. Spare components are available and are completely interchangeable.

Alternative Dounce homogenizers Alternative Homogenisers DUALL®, with PTFE pestle

With conical and cylindrical area for effective tissue for homogenising. The PTFE pestle is ideal for soft tissue such as brain or liver. The pestle is suitable for motor drive. The space between the pestle and mortar is between 0.1mm to 0.15mm. Spare components are available and are completely interchangeable.

Alternative Dounce homogenizers Alternative Homogenisers DUALL®, with glass pestle Alternative Homogenisers Potter-Elvehjem, with glass pestle Alternative Homogenisers Potter-Elvehjem, with PTFE pestle

All-glass. Pestle is suitable for motor drive. The space between the pestle and mortar is between 0.1mm to 0.15mm. Spare components are available and are completely interchangeable.

Alternative Homogenisers Potter-Elvehjem, with PTFE pestle Alternative Homogenisers DUALL®, with PTFE pestle

With PTFE pestle and glass mortar. Sizes 21 and 24 have notches on the bottom edge of the pestle so that particles can be transported up to the cylindrical part of the mortar for extraction. The space between the pestle and mortar is between 0.1mm to 0.15mm. Spare components are available and are completely interchangeable.

Alternative Homogenisers Potter-Elvehjem, with glass pestle Alternative Homogenisers DUALL®, with PTFE pestle

For use with all homogeniser drive-units or for schuett homgen^plus.
Homogenisers and pestles provide a precise shape and are consequently separately exchangeable. Clearance between pestle and homogeniser is between 0.18 and 0.23 mm.

Homogeniser with PTFE pestle
Tapered or cylindrical. Shaft made of stainless steel. Inert. Elastic. With smooth, wax-like, moisture-rejecting surface. Resistant against high temperatures. Also available as glass fiber reinforced model. Highly efficient with especially tough tissue.

Homogeniser with glass pestle
Tapered or cylindrical. With robust glass shaft. Ground-glass surface in cylindrical/tapered part. Highly efficient with tough tissue.

Homogenizers and pestles also available individually as spare parts.

Glass mortar Type 1:	With flared overflow neck and spout, conical
Glass mortar Type 2:	Without flared overflow neck, cylindrical
Glass mortar Type 3:	With flared overflow neck and spout, cylindrical
Glass mortar Type 4:	Round bottom, with flared overflow neck, without spout, cylindrical

Accessories  Accessories Ground Joint GL Adapters, PTFE

For fine particle size reductions without damage to cell nuclei. Supplied with interchangeable pestles ground and polished to large" and small" clearances for use in the same tube. Includes 2 larges pestle used for sample preparation and 2 tight pestles for final homogenate.

The pestles are conicaly shaped at the tip and cylindrically shaped in the upper part. This combination gives two separate areas, one for initial grinding and a second for final homogenisation.

For grinding bacterial emulsions and tissues. Rounded pestle and mortar ends, ensure perfect grinding. Autoclavable. Large Griffiths tube is ideal for coarse material.

Stainless steel handle for Potter-elvehjem homogenisers

Manufactured from high quality borosilicate glass this range of mini homogenisers is ideal for micro tissue work. Bodies are clear precision glass. Pestles have ground glass working length. With or without handles. Fully autoclavable.
Grinding clearance pestle and mortar 0.152mm to 0.254mm.

The all-glass Uni-Form range of round body tissue grinders is made from sterilisable heat-resisting glass in capacities 5ml to 55ml. Models are available with rod ends for motor drive, or hollow pestles designed for use with coolants/dry ice. Bodies are available in clear precision glass or ground glass for more effective homogenisation of tougher tissues.
Grinding clearance pestle and mortar 0.152mm to 0.254mm.

Supplied complete with a PTFE pestle, stainless steel handle and borosilicate glass mortar which is stoppered in PTFE to protect against splash back during the grinding process. Autoclavable.

Accessories  Accessories Disposable Pellet pestles, PP, with reaction tube Accessories Micro pestles, CTFE / stainless steel

Reusable, for 500µl and 1.5ml reaction tubes. Autoclavable CTFE / stainless steel. Suitable for battery-operated drive motor (9.314 496).

Alternative  Alternative Disposable Pellet pestles, PP, with reaction tube Alternative Micro pestles, accessory drive motor

Autoclavable micro-pestle for re-suspending pellets in 1.5 to 2.0 ml containers.

• Potter-elvehjem homogenisers are designed to work with soft tissue
• Precision Bore Borosilicate glass mortars with a range from 5.0 to 55ml capacity
• PTFE Pestle
• Standard stainless steel rod for use with an electric motor
• Optional stainless steel handle for manual grinding
• All parts are fully autoclavable

Grinding Clearance Pestle and Mortar: 0.076 to 0.126mm

Accessories Homogenisers, Handle for Potter-elvehjem

Vivaspin™ ultrafiltration spin columns for minimal handling and reduced sample loss. Specially designed for rapid, non-denaturing concentration and desalting of proteins and other biological samples. Vertical polyethersulfone (PES) membrane minimizes membrane clogging to ensure high flow rates.

Features & Benefits:

• Dead-stop technology: samples are not concentrated to dryness and allows direct concentrate recovery
• pH compatibility from pH 1 to 9
• Polycarbonate tube design for minimal protein adsorption
• Suitable for sample volumes from 100 µl to 20 ml with a molecular weight cutoff value of Mr 3000 to 100 000

Amicon® ultra centrifugal filters promote high sample recovery, even in diluted samples, through ultrafiltration and also provide fast sample processing. Particularly suitable for sensitive downstream applications as the centrifugal filters provide the fastest and most efficient concentration. The Ultracel-50 centrifugal filters have a NMWL (Nominal Molecular Weight Limit) of 50 kDa.

Features & Benefits:

• Prevents turning to dryness
• Provides a predictable concentration factor
• No calibration is necessary to run multiple samples in parallel
• Vertical membrane aligned with filtrate instead of vertical for loss constipation, less waste and faster filtration
• 25 to 80 times the concentration in one step
• Wide chemical compatibility, heat-sealed membrane and compatible with pH 1-9
• Maximum sample recovery as Ultracel® low-bund membrane > 90 % recovery
• Centrifuge your valuable sample with security in a robust, slim unit that prevents leakage

The Millex® Syringe Filter has been specially manufactured for sterilization or clarification of biological solutions. To increase throughput and flow rate, the filter has a larger filtration surface. This also reduces the pressure required to emtpy the syringe. The filters are provided with a quality certification because they are manufactured under a controlled environment and an automated process.

If you want to have free chlorine, large particles and organic compounds removed from your tap water, use the Progard® pre-treatment packs. The reverse osmosis membrane (RO) is protected from chlorine oxidation, mineral scaling and organic pollution.

Features & Benefits:

• Ensures optimization of system life and performance

Silver-impregnated activated carbon for free chlorine reduction and bacteriostatic protection

• Depth filtration for particle removal

Amicon® ultra centrifugal filters promote high sample recovery, even in diluted samples, through ultrafiltration and also provide fast sample processing. Particularly suitable for sensitive downstream applications as the centrifugal filters provide the fastest and most efficient concentration. The Ultracel-3 centrifugal filters have a NMWL (Nominal Molecular Weight Limit) of 3 kDa.

Features & Benefits:

• Prevents turning to dryness
• Provides a predictable concentration factor
• No calibration is necessary to run multiple samples in parallel
• Vertical membrane aligned with filtrate instead of vertical for loss constipation, less waste and faster filtration
• 25 to 80 times the concentration in one step
• Wide chemical compatibility, heat-sealed membrane and compatible with pH 1-9
• Maximum sample recovery as Ultracel® low-bund membrane > 90 % recovery
• Centrifuge your valuable sample with security in a robust, slim unit that prevents leakage

Without any filtrate transfer, you can filter into a 50 ml centrifuge tube with the Steriflip® filter unit. Simply screw the filter unit onto your centrifuge tube, tilt it 180 degrees and apply vacuum. The second tube, which is attached to the filter unit, now contains your sterile filtrate.

High sample recovery by ultrafiltration, even in diluted samples, and fast sample processing are offered by Amicon® ultra-centrifugal filters. The centrifugal filters, for fastest and most efficient concentrations, are specially designed for sensitive downstream applications. The Ultracel-10 centrifugal filters have a NMWL (Nominal Molecular Weight Limit) of 10 kDa.

Features & Benefits:

• Prevents rotation to dryness
• Providing a predictable concentration factor
• No calibration necessary if several samples are to be run in parallel
• Vertical membrane, aligned with filtrate
• Less clogging, faster filtration and less waste

Wide chemical compatibility, heat-sealed membrane and compatible with pH 1-9

• Robust, slim unit that prevents leakage

The Millex® Syringe Filter has been specially manufactured for sterilization or clarification of biological solutions. To increase throughput and flow rate, the filter has a larger filtration surface. This also reduces the pressure required to emtpy the syringe. The filters are provided with a quality certification because they are manufactured under a controlled environment and an automated process.

The nylon-membrane filters are compatible with a wide range of solvents. Its apllication includes particel removal and clarification, solvent filtration and particel analysis.

Features & Benefits

• Autoclavable with filter for sterile filtration
• Hydrophilic

Tubes of 2 ml.

The patented Disruptor Genie^® provides a multi-directional action, which simultaneously agitates and vortexes at high speed, dramatically increasing cell disruption or sample resuspension efficiency.

• Dramatically increased sample disruption efficiency. Ideal for difficult glass bead procedures (Glass beads - please order separately), cell disruption/homogenisation of yeast, bacteria, plant and animal tissues and pellet resuspensions or anything that requires extremely violent, high-speed agitation.
• Patented multi-directional action simultaneously agitates and vortexes at high-speed producing greater yields in less time
• Hands-Free disruption of up to twelve 1.5ml or 2.0ml microtubes with timer from 0 to 15 minutes (Disruptor Genie^® analog), 0 to 99 minutes (Disruptor Genie^® digital) or continuous operation
• Performance comparable to expensive ultrasonic cell disruptors/homogenisers.
• Suitable for use in cold rooms or incubators

Supplied with: Disruptor Genie, tube holder for 12 tubes, pop-off cup

Specifications
Speed Disruptor Genie® analog: Disruptor Genie® digital:	2850rpm 1000 to 2850rpm
Dimensions (W x D x H):	122 x 165 x 190mm
Weight:	4.3kg
Supply requirements:	220V 50Hz, 250mA

Accessories Disruptor beads for Disruptor Genie® / Bead GenieTM Accessories  Accessories

Amicon® ultra centrifugal filters promote high sample recovery, even in diluted samples, through ultrafiltration and also provide fast sample processing. Particularly suitable for sensitive downstream applications as the centrifugal filters provide the fastest and most efficient concentration. The Ultracel-30 centrifugal filters have a NMWL (Nominal Molecular Weight Limit) of 30 kDa.

Features & Benefits:

• Prevents turning to dryness
• Provides a predictable concentration factor
• No calibration is necessary to run multiple samples in parallel
• Vertical membrane aligned with filtrate instead of vertical for loss constipation, less waste and faster filtration
• 25 to 80 times the concentration in one step
• Wide chemical compatibility, heat-sealed membrane and compatible with pH 1-9
• Maximum sample recovery as Ultracel® low-bund membrane > 90 % recovery
• Centrifuge your valuable sample with security in a robust, slim unit that prevents leakage

The easy-to-use, small capacity Thermo Scientific Savant DNA 130 is the ideal choice for nucleic acid applications. The high-performance DNA SpeedVac Concentrator offers rapid and efficient concentration, and drying of small-volume DNA/RNA samples.

• Corrosion resistant, PTFE coated chamber provides years of lasting service
• Built-in, oil free vacuum pump provides maintenance-free operation
• Integrated and compact design
• Extremely quiet operation
• Choice of three drying rates.
• Dual digital timers for independent control of heat and run times
• Solvents and Solvent Combinations - DNA 130: PCR buffers (aqueous), Water, Ethanol and water
• DNA concentrator with integrated pump, plexiglass lid and RD36 rotor for 36 microtubes (1.5 & 2.0 ml)

Specifications
Max. Vacuum:	<10 Torr (13 mbar)
Displacement volume:	30 l/min at 50 Hz
Chamber temperature:	35 ... 65 °C
Ambient temperature:	17 ... 32 °C
Dimension (W x D x H):	64 x 29 x 31 cm
Weight:	39 kg
Power supply:	230 V / 50 Hz

Durapore® PVDF membranes are characterized by high throughput and high flow rate. To maximize recovery and minimize interaction with your sample, the membrane has very low protein binding.

Features & Benefits:

• Can be sterilized by ethylene oxide and gamma irradiation
• Available in different pore sizes

The glass fiber filters are available with and without binder resins and can be sterilized by ethylene oxid, gamma irridation or in the autoclave.

Glass fiber filter with binding resin:

• Superior wet strength
• Qualitative analysis and prefiltration, especially for highly contaminated solutions
• To clarify aqueous solutions
• Type AP15/20/25

S-Pak filters are manufactured from mixed cellulose esters and have been specially optimized for MF methods. Microbiological analysis of water or other liquids.

The Millex® Syringe Filter has been specially manufactured for sterilization or clarification of biological solutions. To increase throughput and flow rate, the filter has a larger filtration surface. This also reduces the pressure required to emtpy the syringe. The filters are provided with a quality certification because they are manufactured under a controlled environment and an automated process.

The Millex® Syringe Filter has been specially manufactured for sterilization or clarification of biological solutions. To increase throughput and flow rate, the filter has a larger filtration surface. This also reduces the pressure required to emtpy the syringe. The filters are provided with a quality certification because they are manufactured under a controlled environment and an automated process.

The Millex® Syringe Filter has been specially manufactured for sterilization or clarification of biological solutions. To increase throughput and flow rate, the filter has a larger filtration surface. This also reduces the pressure required to emtpy the syringe. The filters are provided with a quality certification because they are manufactured under a controlled environment and an automated process.

The Millex® Syringe Filter has been specially manufactured for sterilization or clarification of biological solutions. To increase throughput and flow rate, the filter has a larger filtration surface. This also reduces the pressure required to emtpy the syringe. The filters are provided with a quality certification because they are manufactured under a controlled environment and an automated process.

The Milli-Q Kits: QPAK® contain a combination of purification media (including ion exchange resins, activated carbon and Organex Q™ mixture) carefully selected to eliminate impurities from a wide variety of feed water types.

The stacked-disk format of Millipak® filter units eliminates the risk of fiber release from downstream support material.

Features & Benefits:

• No risk of membrane cracking as the 0.22 µm membrane is supported by a rigid plastic disc with channels
• As the membranes are welded onto the support disc, the risk of contamination is considerably reduced
• Minimum dead volume thanks to the large filter surface

The disposable plastic Petri dishes with inserted sterile cardboard disc are ideally suited for the cultivation of microorganisms. The risk of contamination is minimized, which saves costs and time.

The Milliflex® Quantum high purification columns were developed to eliminate ions and traces of organic substances in ultrapure water.
To prevent operating errors and to ensure full traceability, there is the RFID identification (Radio Frequency Identification).

The SmartPak® for Direct-Q® systems is perfect for extracting ultrapure water from tap water. It combines all cleaning steps in one.

Features & Benefits:

• Activated carbon depth filter
• Reverse Osmosis Cartridge
• Ion exchange resins

The Stericup® vacuum filtration system combines a filtrate collection bottle with a Steritop™ filter unit. The stability of the unit during filtration is improved by the conical shape of the collection bottle. This makes handling much easier. Even for use under laminar flow conditions the slim, compact shape is qualified. Stacking the closed bottles on top of each other is feasible, thanks to the slightly curved bottom.

Features & Advantages:

• Filtration and storage from 150 ml to 1000 ml possible