DNA-elektroforese

BIOSOLUTE® agaroses are characterized by easy dissolution and rapid gelation as well as very low DNA fixation. Our high quality BIOSOLUTE® Agarose products are ideally suited for analytical and preparative routine methods in electrophoresis, such as separation of DNA and RNA fragments as well as PCR products, but also for protein electrophoresis, screening and blotting techniques.

Agarose Standard
For rapid routine separation of DNA and RNA fragments, for PCR products, plasmid preparation and for detection, cloning and transfer techniques.

• Electroendosmosis (EEO) 0.14-0.16
• For efficient separation of nucleic acid fragments from 250 bp to 23 kbp
• Excellent solubility and fast gelling
• High transparency and low background for clear and defined bands

Agarose low electroendosmosis
For blotting applications

• Low EEO (0.05-0.13) ensures high electrophoretic mobility
• Extraordinary mechanical resistance for increased stability and easier handling
• Higher thermal stability due to higher difference between gelling and melting temperature
• Excellent transparency and low absorbance of staining reagents give high gel clearity

For the rapid and safe removal of ethidium bromide from gel-staining solutions. Contains an activated carbon matrix, which quickly and easily removes >99 % of ethidium bromide. Each device can decontaminate up to 10 litres of gel-staining solution. After filtration, the decontaminated solution can be safely poured down the laboratory drain. The extractor funnel device fits most standard laboratory flasks and bottles (33 to 45 mm necks), and the unit includes a cap for storage between uses. Pack of 2

LLG-Agarose is ideal for routine separation of DNA and RNA fragments, as well as PCR products with electrophoresis. High transparency and low background of the agarose gel ensures clear and sharply defined bands.

• Very good dissolution and rapid gel formation
• High gel strength even at low agarose concentrations
• Suitable for agarose concentrations from 0.75 up to 2 %
• Good separation efficiency for nucleic acid fragments between 250 bp and 23 kb
• Free of DNAse and RNAse

Appearance:	White powder
Clarity (NTU):	≤4 (1.5 % gel)
Gelling point:	36 ± 1.5 °C (1.5 % gel)
Melting point:	88 ± 1.5 °C (1.5 % gel)
Gel strength:	≥1000 g/cm2 (1 % gel)
Sulphate:	≤0.15 %

This agaroses are suitable for routine analysis of nucleic acids. They combine tensile strength with low electroendoosmotic effects to achieve high resolving power for a wide size range of nucleic acid fragments. AG agaroses are also very environmentally friendly, being manufactured by a process which excludes organic solvents. Also available as low melting point agarose for nucleic acid recovery and enzymatic applications (Cat. No. 4.658 455) and as High Resolution PCR grade agarose for separation of small DNA fragments of up to 800 bp (Cat. No. 6.270 615). Other packing sizes on request.

Specifications	AG | LMA | HRA
CAS: Appearance:	9012-36-6 | 39346-81-1 | 39346-81-1 White to off-white powder
EEO:	≤ 0,13 % | ≤ 0,1 % | ≤ 0,1%
Gelling Point:	36 °C ±1,5°C (1,5 % gel) | 26-30 °C (1,5 % gel) | ≤ 33 °C (1,5 % gel)
Melting Point:	88 °C ±1,5°C (1,5 % gel) | ≤ 65 °C (1,5 % gel) | ≤ 70 °C (1,5 % gel)
Solubility:	Clear, colourless solution at 1g in 100ml water
Moisture:	< 10 %
Gel Strength:	≥ 1200 g/cm2 (1% gel) | ≥ 200 g/cm² (1% gel) | ≥ 750 g/cm² (1,5% gel)
Sulphate:	< 0,20 % | ≤ 0,1 % | ≤ 0,1 %
DNase, RNase, Protease, Endonuclease:	None Detected

The ready-to-use DNA markers run precisely and produce a sharp, well defined ladder. They are available in six molecular weight ranges. The markers contain high intensity reference bands and may be used to perform size comparisons with DNA molecules ranging from the smallest of PCR fragments to large, linearised cosmid vectors.

• Up to 19 bands
• Isolated from restrictive plasmids
• 6 months stable at room temperature
• 12 months shelf life at 4 °C
• 24 months storable at -20 °C
• Vials of 500 µl

A complete electrophoresis system for fast and easy analysis. For 6 x 6 cm to 12.5 x 12 cm gels.

• Up to 112 samples at once
• Casting set and combs included
• Set voltage, amperage and time
• Timer with end of run audible alert
• Direct connection eliminates leads
• Backlit LCD

Set includes: Enduro™ Gel XL, 1 main casting stand, 4 Micro, 6 x 6 cm gel trays, 1 tray divider, 2 double sided combs (5/8 well)

Specifications:
Output voltage/current	10 to 150V / 10 to 400mA
Timer	0 to 99 hours or continuous
Dimensions (W x D x H)	245 x 170 x 62 mm
Gel Capacity	1 large (12.5 x 12 cm), 2 small (12.5 x 6 cm), 4 micro (6 x 6 cm)
Program memory	Last programmed parameters saved

Dependable performance, intelligent control, for the connection of different chamber systems. Ideal for DNA, RNA and protein electrophoresis as well as blotting.

• Simple to program
• Select constant voltage or constant current mode
• Compact, lightweight and stackable
• Extremely quiet operation

Specifications	Model 300 V
Output voltage range/increments:	2 ... 300 V/1 V 4 ... 500 mA/1 mA 90 W max/na
Timer:	1 min to 99 h 59 min or continuous
Programmable:	No
Dimensions:	190 x 250 x 80 mm
Weight:	2.2 kg
Input voltage:	120-230 V
	Model 250 V
Output voltage range/increments:	5 ... 250 V/1 V 10 ... 3,000 mA/10 mA 1 ... 300 W/1 W
Timer:	1 min to 99 h 59 min or continuous
Programmable:	10 steps/program, 20 program memory
Dimensions:	190 x 250 x 80 mm
Weight:	2.5 kg
Input voltage:	120 ... 230 V

The MultiSUB series of horizontal gel units offers the most versatile solution for DNA and RNA agarose gel electrophoresis currently available. The tanks are injection molded and therefore leak free. They are equipped with high quality platinum electrodes. Each unit comes with a variety of gel tray sizes and colour coded combs providing high flexibility for the user. The casting dams supplied with the units offer a simple and leak free solution for casting the gels.
Gel size: 15 x 15 cm (W x L). This unit is ideal for restriction fragment analysis, sample prep or checking of high number of samples. The MultiSUB Choice offers a high degree of versatility. Two additional tray options are available: 15 x 7 cm and 15 x 10 cm.

• Colour coded combs 0.75 / 1.0 / 1.5 / 2.0 mm
• 3, 4 or 6 comb positions for maximum 210 samples
• Low buffer volume: 500 ml
• Simple gel casting by using dams

Supplied with:
MultiSUB Choice 7: 15 x 7 cm UV tray, 2 x 20 sample combs.
MultiSUB Choice 10: 15 x 10 cm UV tray, 2 x 20 sample combs.
MultiSUB Choice 15: 15 x 15 cm UV tray, 2 x 20 sample combs.
MultiSUB Choice Trio: 15 x 7, 10 and 15 cm UV tray, 2 x 20 sample combs.
MultiSUB Choice Trio 15: 15 x 15 cm UV tray, 2 x 20 sample combs.
MultiSUB Choice Stretch 20: 15 x 20 cm UV tray, 4 x 28 sample combs.
MultiSUB Choice Stretch 25: 15 x 25 cm UV tray, 4 x 28 sample combs.
Each model has an additional loading guides and dams.

Specifications
Unit dimensions (w x l x h):	26.5 x 17.5 x 9 cm 41 x 17.5 x 9 cm (Stretch models)
Max. sample:	15 x 7 cm Tray - 70 Samples 15 x 10 cm Tray - 140 Samples 15 x 15 cm Tray - 210 Samples 15 x 20 cm Tray - 280 Samples 15 x 25 cm Tray - 350 Samples
Buffer volume:	500 ml 1000 ml (Stretch models)

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