DNA-elektroforese

BIOSOLUTE® agaroses are characterized by easy dissolution and rapid gelation as well as very low DNA fixation. Our high quality BIOSOLUTE® Agarose products are ideally suited for analytical and preparative routine methods in electrophoresis, such as separation of DNA and RNA fragments as well as PCR products, but also for protein electrophoresis, screening and blotting techniques.

Agarose Standard
For rapid routine separation of DNA and RNA fragments, for PCR products, plasmid preparation and for detection, cloning and transfer techniques.

• Electroendosmosis (EEO) 0.14-0.16
• For efficient separation of nucleic acid fragments from 250 bp to 23 kbp
• Excellent solubility and fast gelling
• High transparency and low background for clear and defined bands

Agarose low electroendosmosis
For blotting applications

• Low EEO (0.05-0.13) ensures high electrophoretic mobility
• Extraordinary mechanical resistance for increased stability and easier handling
• Higher thermal stability due to higher difference between gelling and melting temperature
• Excellent transparency and low absorbance of staining reagents give high gel clearity

For the rapid and safe removal of ethidium bromide from gel-staining solutions. Contains an activated carbon matrix, which quickly and easily removes >99 % of ethidium bromide. Each device can decontaminate up to 10 litres of gel-staining solution. After filtration, the decontaminated solution can be safely poured down the laboratory drain. The extractor funnel device fits most standard laboratory flasks and bottles (33 to 45 mm necks), and the unit includes a cap for storage between uses. Pack of 2

LLG-Agarose is ideal for routine separation of DNA and RNA fragments, as well as PCR products with electrophoresis. High transparency and low background of the agarose gel ensures clear and sharply defined bands.

• Very good dissolution and rapid gel formation
• High gel strength even at low agarose concentrations
• Suitable for agarose concentrations from 0.75 up to 2 %
• Good separation efficiency for nucleic acid fragments between 250 bp and 23 kb
• Free of DNAse and RNAse

Appearance:	White powder
Clarity (NTU):	≤4 (1.5 % gel)
Gelling point:	36 ± 1.5 °C (1.5 % gel)
Melting point:	88 ± 1.5 °C (1.5 % gel)
Gel strength:	≥1000 g/cm2 (1 % gel)
Sulphate:	≤0.15 %

This agaroses are suitable for routine analysis of nucleic acids. They combine tensile strength with low electroendoosmotic effects to achieve high resolving power for a wide size range of nucleic acid fragments. AG agaroses are also very environmentally friendly, being manufactured by a process which excludes organic solvents. Also available as low melting point agarose for nucleic acid recovery and enzymatic applications (Cat. No. 4.658 455) and as High Resolution PCR grade agarose for separation of small DNA fragments of up to 800 bp (Cat. No. 6.270 615). Other packing sizes on request.

Specifications	AG | LMA | HRA
CAS: Appearance:	9012-36-6 | 39346-81-1 | 39346-81-1 White to off-white powder
EEO:	≤ 0,13 % | ≤ 0,1 % | ≤ 0,1%
Gelling Point:	36 °C ±1,5°C (1,5 % gel) | 26-30 °C (1,5 % gel) | ≤ 33 °C (1,5 % gel)
Melting Point:	88 °C ±1,5°C (1,5 % gel) | ≤ 65 °C (1,5 % gel) | ≤ 70 °C (1,5 % gel)
Solubility:	Clear, colourless solution at 1g in 100ml water
Moisture:	< 10 %
Gel Strength:	≥ 1200 g/cm2 (1% gel) | ≥ 200 g/cm² (1% gel) | ≥ 750 g/cm² (1,5% gel)
Sulphate:	< 0,20 % | ≤ 0,1 % | ≤ 0,1 %
DNase, RNase, Protease, Endonuclease:	None Detected

The ready-to-use DNA markers run precisely and produce a sharp, well defined ladder. They are available in six molecular weight ranges. The markers contain high intensity reference bands and may be used to perform size comparisons with DNA molecules ranging from the smallest of PCR fragments to large, linearised cosmid vectors.

• Up to 19 bands
• Isolated from restrictive plasmids
• 6 months stable at room temperature
• 12 months shelf life at 4 °C
• 24 months storable at -20 °C
• Vials of 500 µl

A complete electrophoresis system for fast and easy analysis. For 6 x 6 cm to 12.5 x 12 cm gels.

• Up to 112 samples at once
• Casting set and combs included
• Set voltage, amperage and time
• Timer with end of run audible alert
• Direct connection eliminates leads
• Backlit LCD

Set includes: Enduro™ Gel XL, 1 main casting stand, 4 Micro, 6 x 6 cm gel trays, 1 tray divider, 2 double sided combs (5/8 well)

Specifications:
Output voltage/current	10 to 150V / 10 to 400mA
Timer	0 to 99 hours or continuous
Dimensions (W x D x H)	245 x 170 x 62 mm
Gel Capacity	1 large (12.5 x 12 cm), 2 small (12.5 x 6 cm), 4 micro (6 x 6 cm)
Program memory	Last programmed parameters saved

Dependable performance, intelligent control, for the connection of different chamber systems. Ideal for DNA, RNA and protein electrophoresis as well as blotting.

• Simple to program
• Select constant voltage or constant current mode
• Compact, lightweight and stackable
• Extremely quiet operation

Specifications	Model 300 V
Output voltage range/increments:	2 ... 300 V/1 V 4 ... 500 mA/1 mA 90 W max/na
Timer:	1 min to 99 h 59 min or continuous
Programmable:	No
Dimensions:	190 x 250 x 80 mm
Weight:	2.2 kg
Input voltage:	120-230 V
	Model 250 V
Output voltage range/increments:	5 ... 250 V/1 V 10 ... 3,000 mA/10 mA 1 ... 300 W/1 W
Timer:	1 min to 99 h 59 min or continuous
Programmable:	10 steps/program, 20 program memory
Dimensions:	190 x 250 x 80 mm
Weight:	2.5 kg
Input voltage:	120 ... 230 V

The MultiSUB series of horizontal gel units offers the most versatile solution for DNA and RNA agarose gel electrophoresis currently available. The tanks are injection molded and therefore leak free. They are equipped with high quality platinum electrodes. Each unit comes with a variety of gel tray sizes and colour coded combs providing high flexibility for the user. The casting dams supplied with the units offer a simple and leak free solution for casting the gels.
Gel size: 15 x 15 cm (W x L). This unit is ideal for restriction fragment analysis, sample prep or checking of high number of samples. The MultiSUB Choice offers a high degree of versatility. Two additional tray options are available: 15 x 7 cm and 15 x 10 cm.

• Colour coded combs 0.75 / 1.0 / 1.5 / 2.0 mm
• 3, 4 or 6 comb positions for maximum 210 samples
• Low buffer volume: 500 ml
• Simple gel casting by using dams

Supplied with:
MultiSUB Choice 7: 15 x 7 cm UV tray, 2 x 20 sample combs.
MultiSUB Choice 10: 15 x 10 cm UV tray, 2 x 20 sample combs.
MultiSUB Choice 15: 15 x 15 cm UV tray, 2 x 20 sample combs.
MultiSUB Choice Trio: 15 x 7, 10 and 15 cm UV tray, 2 x 20 sample combs.
MultiSUB Choice Trio 15: 15 x 15 cm UV tray, 2 x 20 sample combs.
MultiSUB Choice Stretch 20: 15 x 20 cm UV tray, 4 x 28 sample combs.
MultiSUB Choice Stretch 25: 15 x 25 cm UV tray, 4 x 28 sample combs.
Each model has an additional loading guides and dams.

Specifications
Unit dimensions (w x l x h):	26.5 x 17.5 x 9 cm 41 x 17.5 x 9 cm (Stretch models)
Max. sample:	15 x 7 cm Tray - 70 Samples 15 x 10 cm Tray - 140 Samples 15 x 15 cm Tray - 210 Samples 15 x 20 cm Tray - 280 Samples 15 x 25 cm Tray - 350 Samples
Buffer volume:	500 ml 1000 ml (Stretch models)

Accessories Power supplies POWERpro for gel electrophoresis tanks Accessories Power supply nanoPAC-300 Mini for gel electrophoresis tanks Accessories Accessories for Gel Electrophoresis Tank MultiSUB Choice Alternative Agarose gel electrophoresis workflow package choicePACK Alternative Electrophoresis package MSCHOICETRIO-PP300

The MultiSUB series of horizontal gel units offers the most versatile solution for DNA and RNA agarose gel electrophoresis currently available. The tanks are injection molded and therefore leak free. They are equipped with high quality platinum electrodes. Each unit comes with a variety of gel tray sizes and colour coded combs providing high flexibility for the user. The casting dams supplied with the units offer a simple and leak free solution for casting the gels.
The MultiSUB Maxi is primarily designed for resolution of high numbers of samples such as from Cloning or PCR. The MultiSUB Maxi allows ultra high-resolution separations over extended runs. Tray sizes correspond to standard blotter sizes. It also allows easy sample transfer onto a membrane for further analysis. Four gel tray sizes are available: 20 x 10 cm, 20 x 15 cm, 20 x 20 cm and 20 x 25 cm. Multichannel pipette compatible, 40 combs facilitates speed loading of up to 440 samples per gel. 50 sample combs allow maximum sample capacity of 550 samples per gel.

Supplied with:
MultiSUB Maxi 15: 20 x 15 cm UV Tray, 2 x 20 sample, 1 mm thick combs.
MultiSUB Maxi 20: 20 x 20 cm UV Tray, 2 x 20 sample, 1 mm thick combs.
MultiSUB Maxi DUO: 20 x 10 + 20 x 20 cm UV Tray, 2 x 20 sample, 1 mm thick combs.
MultiSUB Maxi 25: 20 x 25 cm UV Tray, 2 x 20 sample, 1 mm thick combs.
Each model has an additional casting dams and loading guides.

Specifications
Gel dimensions (w x l):	10 x 7 cm and 10 x 10 cm
Unit dimensions (w x l x h):	22 x 12.5 x 9 cm
Max. sample capacity:	20 x 10 cm tray, 200 samples 20 x 15 cm tray, 350 samples 20 x 20 cm tray, 450 samples 20 x 25 cm tray, 550 samples
Buffer volume:	1500 ml

Accessories Power supplies POWERpro for gel electrophoresis tanks Accessories Power supply nanoPAC-300 Mini for gel electrophoresis tanks Accessories Accessories for Gel Electrophoresis Tank MultiSUB Maxi Alternative Electrophoresis package MSMAXIDUO-PP300

The MultiSUB series of horizontal gel units offers the most versatile solution for DNA and RNA agarose gel electrophoresis currently available. The tanks are injection molded and therefore leak free. They are equipped with high quality platinum electrodes. Each unit comes with a variety of gel tray sizes and colour coded combs providing high flexibility for the user. The casting dams supplied with the units offer a simple and leak free solution for casting the gels.
Extending only the width of this unit allows more samples to be resolved per gel than the MultiSUB Mini without a significant increase in buffer or gel volumes. A maximum of 100 samples per gel can be resolved making this unit ideal for those routinely checking medium numbers of samples over short to medium gel run lengths.

Supplied with:
MultiSUB Midi 7: 10 x 7 cm UV tray, 2 x 16 sample combs.
MultiSUB Midi 10: 10 x 10 cm UV tray, 2 x 16 sample combs.
MultiSUB Midi DUO: 10 x 7 cm + 10 x 10 cm UV tray, 2 x 16 sample combs.
Each model has additional loading guides and dams.

Specifications
Gel dimensions (W x L):	10 x 7 cm and 10 x 10 cm
Unit dimensions (W x L x H):	22 x 12.5 x 9 cm
Max. sample capacity:	10 x 7 cm tray, 50 samples 10 x 10 cm tray, 100 samples
Buffer volume:	300 ml

Accessories Power supplies POWERpro for gel electrophoresis tanks Accessories Power supply nanoPAC-300 Mini for gel electrophoresis tanks Accessories Accessories for Gel Electrophoresis Tank MultiSUB Midi Alternative Agarose gel electrophoresis workflow package miniPACK Alternative Electrophoresis package MSMIDIDUO-NANO300

The MultiSUB series of horizontal gel units offers the most versatile solution for DNA and RNA agarose gel electrophoresis currently available. The tanks are injection molded and therefore leak free. They are equipped with high quality platinum electrodes. Each unit comes with a variety of gel tray sizes and colour coded combs providing high flexibility for the user. The casting dams supplied with the units offer a simple and leak free solution for casting the gels.
The MultiSUB Midi-96 Gel System is ideal for high throughput electrophoresis of PCR products or samples containing a small number of DNA bands. Its 10 x 12 cm (W x L) gel dimensions and 96-well comb block format correspond to the standard micro plate configuration, with additional lanes for markers. Two different types of comb are available: one which has 8 wells and one additional lane for DNA markers and one which has 8 wells and 2 additional lanes for markers. The offset layout of the wells allows a maximum run length of 1.8 cm per well, resulting in improved separation. MultiSUB Midi 96 can accommodate samples from a 96-well plate; samples can be loaded directly using 8-channel pipettes. As with all MultiSUB units, leak-proof casting is simplified using the supplied casting dams while their ultra-compact size minimises buffer usage and bench space requirements. MultiSUB Midi-96ST (Stretch) Systems are also available for those users requiring an extended run length per well of up to 3.6 cm.

Supplied with:
MultiSUB Midi-96: UV tray, Comb block with 12 x 8 sample, 1 mm thick combs, casting dams.
MultiSUB Midi-96/2M: UV tray, Comb block with 12 x 8 sample, 1 mm thick combs, casting dams. Combs have two marker lanes.
MultiSUB Midi-96ST: STRETCH, UV tray, Comb block with 12 x 8 sample, 1 mm thick combs, casting dams.
MultiSUB Midi-96ST/2M: STRETCH, UV tray, Comb block with 12 x 8 sample, 1 mm thick combs, casting dams. Combs have two marker lanes.

Specifications	MSMIDI96 / MSMIDI96ST
Gel dimensions (W x L):	10 x 12 cm / 10 x 24 cm
Unit dimensions (W x D x H):	22 x 12.5 x 9 cm / 46.5 x 12.5 x 8 cm
Max. sample capacity:	96 samples plus 1 or 2 marker lanes
Buffer volume:	300 ml / 700 ml

Accessories Power supplies POWERpro for gel electrophoresis tanks Accessories Power supply nanoPAC-300 Mini for gel electrophoresis tanks Accessories Accessories for Gel Electrophoresis Tank MultiSUB Midi-96 Alternative Electrophoresis package MSMIDI96-PP300

The MultiSUB series of horizontal gel units offers the most versatile solution for DNA and RNA agarose gel electrophoresis currently available. The tanks are injection molded and therefore leak free. They are equipped with high quality platinum electrodes. Each unit comes with a variety of gel tray sizes and colour coded combs providing high flexibility for the user. The casting dams supplied with the units offer a simple and leak free solution for casting the gels.
The MultiSUB Mini is the smallest unit in the range, designed for low to medium numbers of samples. The cost-effective small gel size does not compromise versatility as two tray options are available: 7 x 7 cm and 7 x 10 cm. Easy preparation up to 16 samples. Simply by altering the gel tray or comb, this compact unit is capable of resolving up to 64 different samples, prepping 1ml of sample or separating sample bands over a distance of 9 cm.

Supplied with:
MultiSUB Mini 7: 7 x 7 cm UV Tray, 2 x 8 sample combs.
MultiSUB Mini 10: 7 x 10 cm UV Tray, 2 x 8 sample combs.
MultiSUB Mini DUO: 7 x 7 cm and 7 x 10 cm UV Tray, 2 x 8 sample combs.
Set: MultiSUB Mini DUO + Power supply OmniPAC, MINi 300 V 400mA 60 W, 110/230 V.
Each model has an additional loading guides and dams.

Specifications
Gel dimensions (W x L):	7 x 7cm and 7 x 10 cm
Unit dimensions (W x L x H):	21 x 9 x 9 cm
Max. sample capacity:	7 x 7 cm tray, 32 samples 7 x 10 cm tray, 64 samples
Buffer volume:	225 ml

Accessories Power supplies POWERpro for gel electrophoresis tanks Accessories Power supply nanoPAC-300 Mini for gel electrophoresis tanks Accessories Accessories for Gel Electrophoresis Tank MultiSUB Mini Alternative Electrophoresis package MSMINIDUO-NANO300

The MultiSUB series of horizontal gel units offers the most versatile solution for DNA and RNA agarose gel electrophoresis currently available. The tanks are injection molded and therefore leak free. They are equipped with high quality platinum electrodes. Each unit comes with a variety of gel tray sizes and colour coded combs providing high flexibility for the user. The casting dams supplied with the units offer a simple and leak free solution for casting the gels.
The MiniRapide is a completely self contained system designed for quick checks of samples. Gel casting, running and analysis are all performed in the same ultra compact unit. Buffer and gel volumes have been kept to a minimum and the parallel electrode arrangement allows ultra efficient current transfer, enabling resolution to be completed within 30 minutes. The UV transparent base allows direct viewing on a UV Transilluminator with no need for time consuming transfer and potential gel damage. Dual comb slots allow the loading of up to 40 samples per gel while multichannel pipette compatible combs further enhance the speed and convenience.

Scope of supply: Gel electrophoresis tank with lid, 2 x 8 sample combs 1.5 mm, electrodes, casting dams

Specifications
Gel dimensions (W x L):	10 x 8 cm
Unit dimensions (W x L x H):	15 x 15 x 4 cm
Max. sample capacity:	40 samples
Buffer volume:	50 ml

Accessories Accessories for Gel Electrophoresis Tank MultiSUB MiniRapide

The Mini-Blue light Transilluminator uses harmless blue light to illuminate both traditional Ethidium bromide stained gels as well as safe stains. Slotting under any gel tank, but perfect for our MINI and MIDI sized multiSUB gel tanks. With an orange filter window to visualize DNA in real time within gel tanks, it is perfect for quickly checking PCR fidelity and restriction digest results. The device can also be used as a stand-alone transilluminator with the gel placed directly on the illuminator.

• High intensity blue LED illuminator allows visualization of a wide range of fluorescent gel stains
• Compatible with runSAFE, commercial safe stains and ethidium bromide

Scope of supply: Transilluminator, filter (orange)

Specifications
Wavelength:	470 nm
Viewing size:	112 x 75 mm
Dimensions (W x D x H):	86 x 170 x 25 mm
Weight:	338 g
Power supply:	DC 12 V, 2 A

Alternative Blue- and white light LED-Transilluminators

The nanoPAC-300 is capable of running all Cleaver Scientific horizontal multiSUB™ systems and vertical omniPAGE mini gel systems.

• Maximum constant current output of 400mA
• Simple two step feature to set a programmable change in voltage, current and time during the run
• Constant voltage up to 300 V
• Adjustable in 1 V and 1 mA increments
• Two parallel outputs
• Continuous run or timed setting up to 999 minutes
• Automatic safety shutdown
• Alarm function
• Easy-to-clean polycarbonate housing
• 3-digit LED display

4 output terminals to allow up to 4 simultaneous runs. Powerful microprocessor control allows complex programming, while manual mode permits the setting of voltage, current, power and time for routine electrophoretic runs. The parameters may also be changed temporarily without interrupting the run.

• LCD display
• Data logging
• Real time clock

Programs:	9 programs, 9 steps each
Resolution: Interface:	1 V, 1 mA, 1 W USB
Max. voltage:	300 V
Max. current:	2000 mA
Max. power:	300 W
Timer:	0 ... 99:59 h
Dimensions (W x D x H):	310 x 260 x 130 mm
Weight:	10 kg

• Versatile, self-contained system on the market for real-time horizontal gel electrophoresis
• System includes everything except chemicals and reagents to run and visualize horizontal SYBR- and EtBr-stained gels in real-time
• Combines the flexibility and high resolution capability of the MSCHOICE system with the time- and space saving convenience of having the power supply and gel illuminator integrated within one highly compact bench top unit
• Optional dark room with 24.1 megapixel digital camera fits directly over the base unit and gel tank to allow gels to be imaged either as bands migrate in real-time or at the end of the electrophoresis run
• Includes accessories for standard gel electrophoresis and for size fractionation and simplified real-time gel free DNA band extraction and purification
• runVIEW Gel System with bluVIEW Lid Design: Orange spectral emission filter with condensation-free viewing panel.
• Extractor fan in the bluVIEW lid eliminates condensation to optimise DNA visualisation and resolution during electrophoresis.

Included Double-sided Combs: 2x 1-sample / 2-sample preparatory; 2x 4-sample preparatory / 16-sample combs; 4x 20- /28- sample multichannel compatible screening. Comb Thickness: 1mm. Extra-thick 2x 4- and 2x 6-sample 3mm preparatory combs are also included.

Specifications
runVIEW Viewing Dock
Blue Light Wavelength:	470 nm
Voltage / Resolution:	25-150 V / 1 V
Current / Resolution:	300 mA / 1 mA
Power:	30 W
Timer:	1 to 999 min (with alarm)
Dimensions (W x D x H):	29.3 x 22.0 x 8.0 cm
Power supply:	100-240 V, 50/60 Hz
runVIEW Gel System
Gel Dimensions (W X L):	15 x 7, 15 x 10 and 15 x 15 cm
Unit dimensions (W X D X H):	26.5 x 17.5 x 9.0 cm
Buffer volume:	500 ml

The Alpha Cycler family form PCRmax provides a range of features which are specifically designed to meet the needs of modern researchers when amplifying DNA. Given the flexibility in format and block options (96 or 384 well) scaleability is core in the Alpha Cycler range. Alpha Cycler software has features such as recently used programmes, allowing users to quickly access their most commonly used protocols without the need to navigate through folders to find it, individual user logins, with protected protocols. The Alpha Cycler systems also contain a novel programme wizard which allows users to define a protocol based specifically on their primer sequence and template source. Primer sequences or T_M's are entered, amplicon length and source defined and the on board algorithm generates an optimised protocol for your assay. The Alpha Cyclers range of block options allow for use of the most commonly used consumables from different manufacturers and includes 96 well plates (0.2 ml), tubes (0.2 ml), strip tubes (0.2 ml) and 384 well plates.

• Single, dual or four block options available with either 96 or 384 well gradient blocks
• Multi-block units have totally independent blocks for maximum flexibility
• HD Android driven touchscreen interface for simplified programming
• Generate and send instrument reports to diagnose the health of your system and identify any issues before they impact users work
• Lock programs with user defined password
• Alpha Track mobile app allows remote monitoring of run progress

Alpha Track App (free of charge): Track the progress of runs and health of your system remotely via the mobile app
The Alpha Track Application is used in conjunction with your Alpha Cycler and allows users to:

• Remotely track the progress of runs
• Send a health report for diagnosis by the technical support team
• Be notified of any issues and prevent system failures before occurring

Specifications
Block temperature:	10 to 100 °C
Block uniformity at 55 °C:	±0.3 °C
Temperature accuracy at 55 °C:	±0.25 °C
Max. Heating rate:	3.4 °C/s

Complete package for routine applications of agarose gel electrophoresis in the laboratory.

• Electrophoresis tank multiSUB Choice offers a selection of 3 gel tray sizes (15 x 7 cm, 15 x 10 cm and 15 x 15 cm) and can thus be used flexibly
• Power supply PowerPro can be operated in constant mode or programmed and has 5 output pairs

Scope of supply: Electrophoresis tank multiSUB Choice TRIO, power supply PowerPro 300, UV fluorescent ruler, UV gel scoop (15 cm), agarose (100 g), DNA ladder (100 bp to 10 Kb), DNA staining reagent runSAFE (50 bp to 20 Kb), TAE running buffer (50 x 1 l)