Culture cellulaire Boîtes de Pétri

Toutes les boîtes de culture cellulaire LABSOLUTE® sont fabriquées en PS transparent de haute qualité, ce qui leur permet d’être utilisées en microscopie. Le traitement de surface permet aux cellules d’adhérer de manière uniforme et régulière.

• Échange gazeux efficace
• Facilement empilable
• Boîtes de 60 mm et 100 mm avec anneau de préhension
• Traitement de surface par plasma de gaz sous vide
• Stérilisé aux rayons gamma (SAL 10^-6)

The standard round design is supplemented by a square-profile dish and the OneWell Plate™. To enable parallel testing of a single sample using different agars, divided dishes with two or three compartments are available. The product range is completed by contact dishes and graduated germ count dishes.

Proporties:

• Available in different nominal sizes
• Easy stacking
• Vented for improved gas exchange
• non-vented for long incubation period
• Sterilised dishes available on request
• Manufactured from crystal clear polystyrene
• Heat resistance for use with hot agar

Traitement de surface pour tous les domaines de la culture cellulaire dans différentes tailles et versions avec une excellente qualité optique et une planéité pour une bonne aptitude à la microscopie. La modification de surface Nunclon™Δ est certifiée apyrogène et testée avec des lignées cellulaires primaires et immortalisées pour la formation de monocouches ainsi que l'efficacité du clonage. Avec couvercle.

Cell culture multiwell plates are available in the hydrophilic surface (TC surface treatment) for improved cell adhesion and hydrophobic surface for suspension cultures and hybridoma cells. Cell culture microplates from the CELLSTAR® range are specifically treated. This treatment leads to polar groups such as carboxyl and hydroxyl groups , added to the plastic surface. These allow the growth of adherent cell lines. They are also suitable for automated systems and contain an alphanumeric well coding. They are also suitable for footprint systems and contain an alphanumeric well coding.

Properties:

• High clarity enables optimal microscopic examinations
• Lid enables optimal gas exchange with the lowest possible evaporation
• Stackable
• Alphanumeric well coding
• Individually wrapped peel-off bags
• Consecutive lot numbering
• 384 and 1536 well foramt available

CELLSTAR® Cell Culture Dishes are made of high-grade polystyrene. Improved cell adhesion through physical surface treatment. The special design of our cell culture dishes makes it possible to efficiently reach the cell lawn with a cell scraper. The dishes are available in different sizes and suitable surface.

Properties:

• Improved cell adhesion through physical surface treatment
• Vents ensure optimal gas exchange
• Sterile and user-friendly packaging
• Available in the nominal sizes 35, 60, 100 and 145 mm ø
• 8.7 to 143 cm² growth areas
• Easy stacking
• Maximal transparency for excellent microscopic analysis
• 35 ø dish also available with 4 internal wells

Cell culture multiwell plates are available in the hydrophilic surface (TC surface treatment) for improved cell adhesion and hydrophobic surface for suspension cultures and hybridoma cells. Cell culture microplates from the CELLSTAR® range are specifically treated. This treatment leads to polar groups such as carboxyl and hydroxyl groups , added to the plastic surface. These allow the growth of adherent cell lines. They are also suitable for automated systems and contain an alphanumeric well coding. They are also suitable for footprint systems and contain an alphanumeric well coding.

Properties:

• High clarity enables optimal microscopic examinations
• Lid enables optimal gas exchange with the lowest possible evaporation
• Stackable
• Alphanumeric well coding
• Individually wrapped peel-off bags
• Consecutive lot numbering
• 384 and 1536 well foramt available

The CELLCOAT® product line comprises cell culture vessels which are coated with proteins of the extracellular matrix or synthetic proteins. Beside an improved adhesion and proliferation of primary cells and various cell lines. CELLCOAT® plates are highly suitable for serum-free and serum-reduced cell cultivation. Experiments which include additional washing steps or stressful procedures, e.g. transfection. Moreover, the differentiation of individual cell types can be enhanced through the protein-coating.
Collagen Type I is a protein of the extracellular matrix, an intercellular substance which in vivo influences adhesion, migration and proliferation among other processes. Collagen Type I represents one of the most important ECM proteins for in-vitro cell cultures.

Proporties:

• difficult-to-cultivate cells adhere show a positive growth behaviour
• positive influence on differentiation and morphology
• Cell culture flasks with filter caps
• Shelf life: 24 months at room temperature
• Further cell culture vessels coated with Collagen Type I available on request

The CELLCOAT® product line comprises cell culture vessels which are coated with proteins of the extracellular matrix or synthetic proteins. Beside an improved adhesion and proliferation of primary cells and various cell lines. CELLCOAT® plates are highly suitable for serum-free and serum-reduced cell cultivation. Experiments which include additional washing steps or stressful procedures, e.g. transfection. Moreover, the differentiation of individual cell types can be enhanced through the protein-coating.
Collagen Type I is a protein of the extracellular matrix, an intercellular substance which in vivo influences adhesion, migration and proliferation among other processes. Collagen Type I represents one of the most important ECM proteins for in-vitro cell cultures.

Proporties:

• difficult-to-cultivate cells adhere show a positive growth behaviour
• positive influence on differentiation and morphology
• Cell culture flasks with filter caps
• Shelf life: 24 months at room temperature
• Further cell culture vessels coated with Collagen Type I available on request

Nunc™ EasYDisch™ améliore la manipulation, l'empilement et le transport des coupelles de culture. Un anneau de préhension en biais facilite la manipulation même avec des gants et le design breveté de l'encoche garantit une aération maximale avec une stérilité inchangée. Un bord extérieur réhaussé sur le couvercle de la coupelle permet un empilement très stable et plus de sécurité dans le transport. Des marques d'orientation sur le fond de la coupelle facilitent la localisation de cellules sous le microscope.

• Traitement de surface standard Nunclon™Δ, pour un maximum d'adhésion pour la plupart des types de cellules
• Sans RNase/DNase, sans ADN humain
• Non cytotoxique, non pyrogène
• Irradiation gamma (SAL de 10^-6)
• Conservation 5 années à partir de la date de fabrication
• Avec certificat qualité

Le support UpCell™ permet la dissociation rapide des cellules par simple réduction de la température de 37 °C à en dessous de 32 °C.

• Sans trypsinisation vous obtiendrez des cellules avec des protéines de surface intactes pour la culture, l'analyse et la transplantation de cellules
• Pas de grattage des cellules pour conserver leur viabilité
• Les couches de cellules récupérées peuvent être empilées afin de créer des modèles de tissu tridimensionnels et des co-cultures
• Couvercles avec ergots de ventilation
• Certifié fonctionnel, stérile, apyrogène et non-toxique

Le support UpCell™ permet la dissociation rapide des cellules par simple réduction de la température de 37 °C à en dessous de 32 °C.

• Sans trypsinisation vous obtiendrez des cellules avec des protéines de surface intactes pour la culture, l'analyse et la transplantation de cellules
• Pas de grattage des cellules pour conserver leur viabilité
• Les couches de cellules récupérées peuvent être empilées afin de créer des modèles de tissu tridimensionnels et des co-cultures
• Couvercles avec ergots de ventilation
• Certifié fonctionnel, stérile, apyrogène et non-toxique

Plaque à surface traitée pour culture cellulaire dans de nombreuses tailles et formats optiquement clairs et appropriés à la microscopie. La surface de modification de Nunclon est certifiée non-pyrogène et testée pour la formation en monocouche et le clonage en utilisant les cellules primaires aussi bien que des lignes cellulaires répétées. Avec couvercle.

The CELLCOAT® product line comprises cell culture vessels which are coated with proteins of the extracellular matrix or synthetic proteins. Beside an improved adhesion and proliferation of primary cells and various cell lines. CELLCOAT® plates are highly suitable for serum-free and serum-reduced cell cultivation. Experiments which include additional washing steps or stressful procedures, e.g. transfection. Moreover, the differentiation of individual cell types can be enhanced through the protein-coating.
Poly-D-Lysine (PDL) are synthetic molecules that are used to improve adhesion of different cell types to polystyrene surfaces. Especially when serumfree or serum-reduced medium is used or when experiments such as transfections are performed, the cultivation efficiency of individual cell lines can be improved. As synthetic molecule Poly-D-Lysine is free of impurities carried by other proteins.

Proporties:

• Shelf life: 24 months at room temperature
• Cell differentiation and neuron growth
• Automated cell culture

ThinCert™ Cell Culture Inserts for advanced cell and tissue culture applications. They are compatible with standard CELLSTAR® multiwell plates. The coupling between the housing and the membrane is achieved using an automated process which produces an extremely strong and robust seal without compromising or weakening the membrane in any way. The membranes undergo a physical surface treatment to optimise cellular adherence and growth characteristics. Due to a high chemical resistance to solvents a broad spectrum of cell fixation protocols can be performed. The specific hanging geometry design of the ThinCertTM cell culture inserts ensures that there is always a gap between the membrane support and the bottom of the well.

Proporties:

• Stable clear polystyrene housing, USP class VI certified and cell culture compatible
• Hanging geometry
• Sealed PET capillary pore membrane
• Single, sterile blister packing
• Improved cell adhesion through physical surface treatment
• Simplified pipetting due to self-lift geometry
• Enhanced pipetting access and gas exchange

Cell culture multiwell plates are available in the hydrophilic surface (TC surface treatment) for improved cell adhesion and hydrophobic surface for suspension cultures and hybridoma cells. All panels can be stacked together, both with and without cover. The special design of the panels is also in stacked plates a faster heat exchange over the entire bottom surface of each plate allows . All plates have an imprinted lot number and are both pyrogens and DNase / RNase - free .

Properties:

• High clarity and low autofluorescence
• Lid enables optimal gas exchange with the lowest possible evaporation
• Single position lids to prevent cross-contamination
• Alphanumeric well coding
• Compatible with common instruments and automated systems
• Easy-to-open packaging

Coupelles avec surface traitée, adaptées aux cellules adhérentes.

• Faciles à empiler, en sécurité
• Stérilisées par irradiation gamma
• Apyrogènes

Non-binding surfaces from Greiner Bio-One are achieved through a stable chemical modification to covalently link functional groups with the base polystyrene polymer. Under aqueous assay conditions a hydrate layer forms, preventing dissolved biomolecules from binding to the microplate surface. As the non-binding surface is stable under common assay conditions, there is no potential for degradation or leaching and resultant assay interference.

Properties:

• Äußerst geringe, nicht-spezifische Biomolekülbindung (Proteine, DNA, RNA)
• Langfristige Oberflächenbeständigkeit ohne Degeneration oder Auswaschen
• Hohe Testsensitivität mit reduziertem Hintergrund

For the propagation of fastidious cells like primary or sensitive cells as well as cells cultivated under restricted growth conditions. Furthermore, the Advanced TC™ surface facilitates consistent and homogenous cell attachment increasing the overall cell yield and reducing cell loss, for example during automated washing steps. Moreover, cells cultivated on the Advanced TC™ surface exhibit higher transgene activity after gene transfer / insertion. Due to the production process, the modification of the polymer assures consistant and reproducible product quality. Transport and storage can be carried out at room temperature.

Proporties:

• For particularly demanding cells
• Consistent cell attachment
• Homogenous cell growth, for example during automated washing steps
• non-cytotoxic and free of detectable DNase, RNase and human DNA and endotoxins

Stériles, UC=500.

Produits spéciaux pour utilisation en fertilisation in vitro.

The cell culture dishes of CELLSTAR® convince by their improved cell adhesion thanks to a physical surface treatment. The aeration cams ensure an optimal gas exchange. The good stackability and the maximum transparency, for a very good microscopy work, simplify the work enormously. Triple-packed to meet strict cleanroom conditions.

Features & Benefits:

• Free of detectable DNase, RNase and human DNA
• Free of detectable endotoxins, free of cytotoxic substances

The CELLview™ cell culture dish combines the convenience of a standard size 35 mm disposable plastic cell culture dish with the optical quality of glass. Moreover, the dish bottom configuration facilitates optimal thermal conductivity and avoids thermal variations in heated platforms used for live cell imaging. The subdivided version of the CELLview™ Dish enables simultaneous multiplex analyses. Quartering the cell culture dish provides four individual compartments with a growth area of approximately 1.9 cm². In addition to the untreated glass surface, Greiner Bio-One provides a tissue culture surface treatment as well as the innovative Advanced TC™ surface modification to enhance the attachment of adherent cells, thus eliminating the need for protein coating in many cases.

Proporties:

• Subdivided version enables simultaneous multiplex analysis
• Embedded glass bottom for maximal planarity
• Additional TC surface treatment and Advanced TC™ surface modification available
• High transparent achromatic borosilicate glass
• Glass thickness 175 μm +/- 15 μm
• Maximal spectral transmission; no autofluorescence

Coupelles avec surface non traitée, adaptées à la multiplication de cellules en suspension et de tissus.

• Faciles à empiler en sécurité
• Stérilisées par irradiation gamma
• Apyrogènes

Dishes with non-treated surface for a variety of experimental requirements in the cell culture routine.

• Stacking rings on top and bottom for easy and safe stacking
• With lid and air vents
• Sterile
• Pyrogen-free

Plaque à surface traitée pour culture cellulaire dans de nombreuses tailles et formats optiquement clairs et appropriés à la microscopie. La surface de modification de Nunclon est certifiée non-pyrogène et testée pour la formation en monocouche et le clonage en utilisant les cellules primaires aussi bien que des lignes cellulaires répétées. Avec couvercle.