Protein purification

Membrane: polyethersulphone. Vivaspin 20ml centrifugal concentrators have been developed to offer increased volume flexibility and performance. Vivaspin 20 handles up to 20ml in swing bucket centrifuges and 14ml in 25° fixed angle rotors accepting 50ml centrifuge tubes. Featuring twin vertical membranes for unparalleled filtration speeds the Vivaspin 20 can achieve 100x plus concentrations. Remaining volume is easy to read off the printed scale on the side of the concentrator and the modified dead stop pocket further simplifies direct pipette recovery of the final concentrate.

Membrane: polyethersulphone. Compatible with swing-out and fixed-angle rotors. The vertical, built-in membrane guarantees minimal membrane fouling and provides rapid sample concentration. Low protein adsorption gives minimal sample loss.

Membrane: polyethersulphone. Vivaspin 6ml concentrators have been developed to offer increased volume flexibility and performance. Vivaspin 6 can process an impressive 6ml in either swing-out buckets or fixed angle rotors accepting standard 15 ml conical bottom test tubes. The Vivaspin 6 features twin vertical membranes for unparalleled filtration speeds and 100x plus concentrations.

Membrane: polyethersulphone. This 2 ml volume unit combines the rapidity of the classic Vivaspin products with reduced internal surface and membrane areas for superior recoveries from very dilute solutions. With a choice of suitable membranes (PES, CTA, Hydrosart) Vivaspin 2 provides the highest flexibility for process optimisation. Vivaspin 2 guarantees maximum sample recovery either by reverse centrifuging into the concentrate recovery cap or by directly pipetting the concentrate from the deadstop pocket built into the bottom of the concentrator.

Vivaspin^® Filtrate is a ready to use unit for small volume centrifugal ultrafiltration to separate proteins from low molecular weight substances in biological samples.

Typical applications include:

• Drug binding studies
• Determination of metabolites in serum
• Protein removal from blood samples
• Cleaning of liposomes (virus removal)

Vivaspin™ ultrafiltration spin columns for minimal handling and reduced sample loss. Specially designed for rapid, non-denaturing concentration and desalting of proteins and other biological samples. Vertical polyethersulfone (PES) membrane minimizes membrane clogging to ensure high flow rates.

Features & Benefits:

• Dead-stop technology: samples are not concentrated to dryness and allows direct concentrate recovery
• pH compatibility from pH 1 to 9
• Polycarbonate tube design for minimal protein adsorption
• Suitable for sample volumes from 100 µl to 20 ml with a molecular weight cutoff value of Mr 3000 to 100 000

Membrane: cellulose triacetate

Solutions of any macromolecules, simple and efficient, desalt and concentrate. Suitable for any centrifuge holding 1.5 ml tubes.

Features & Benefits:

• Even with diluted solutions the recovery is more than 95%.
• Reverse spin to maximize recovery even in the smallest sample
• Storage of filtrate or concentrated sample, very easy in standard microcentrifuge tubes
• concentration factor up to 100 x

Thin channel, crossflow filter units with 50cm² / 200cm² filter areas for capacities between 100ml / 500ml and 5L. This system provides high crossflow rates at minimum pumping pressure and does not require additional fittings for scale-up. VivaFlow 50 is easy to operate with a flow restrictor and, optionally, a pressure indicator. Modular construction with luer connections allows interlocking of units in series for easy scale-up. Recovery rates are maximised due to optimised recirculation volume and by use of low adsorption materials. Disposable.
For greater volumes, VivaFlow 200 is available with a filter area of 200cm² and includes pressure indicator, flow restrictor, filtrate tubing and connectors.
Membrane: polyethersulphone

Standard and Weighted Spectra/Por Closures provide a leak-proof seal for dialysis tubing while aiding in sample buoyancy and vertical orientation. They are available in 23, 35, 55, 75 and 90 mm sealing widths and a variety of colors for easy sample identification. Closures are autoclavable.

Vivaspin^® 100 is a disposable ultrafiltration unit for the concentration of samples up to 98 ml that can be used either in a centrifuge or for pressure filtration.

• Vertical PES membrane for fast concentration
• Compatible with swing-out rotors

Membrane: Hydrosart^®. Suitable for the concentration of DNA and proteins. The horizontal membrane design was specially developed for the concentration of linear molecules. With Vivacon^® 500, 100 to 500 µl sample volume can be processed in a fixed-angle rotor. Complete sample recovery by reverse spin possible.

Vivacon^® 500 PCR is additionally sterilised with ethylene oxide and free of human DNA.

Ready-to-use crossflow filter units for protein sample concentration, concentration of culture supernatants, virus and water sample concentration. Single units concentrates 250 ml by a factor of 20 in less than 30 min. For speeding up the concentration, two cassettes can be run simultaneously, therfore the concentration of 1000 ml is achieved in about 45 min.

• 50 cm² filter areas
• For volumes 100 - 1000 ml
• Reusable
• High flow rate and recovery

Membrane: Hydrosart^®. With Vivaspin 15R, 2 to 15 ml volumes can be processed in either a swing-out or fixed-angle rotor. The concentrator features very high sample recovery and low adsorption.

The ultrafiltration unit is designed for volumes from 15 ml … 100 µl. The vertical twin membranes enable fast and high sample concentration and almost complete sample recovery. The pipette-friendly dead-stop reservoir eliminates the risk of centrifuging the concentrate to dryness and allows easy recovery of the highly concentrated sample. Samples up to 15 ml can be processed in swing-out rotors or 11 ml in fixed-angle rotors, which are suitable for 50 ml centrifuge tubes with a conical bottom.

• Up to 100 times sample concentration
• High flow rates due to PES membranes
• Volume graduation for easy reading

The ultrafiltration unit is designed for volumes from 4 ml to 40 µl. Its vertical twin membranes enable fast and high sample concentration and almost complete sample recovery. The integrated dead-stop reservoir prevents the concentrate from being centrifuged until dry. Thanks to the pipette-friendly access, the highly concentrated sample can be easily recovered.

• Up to 100 times sample concentration
• High flow rates due to PES membranes
• Compatible with swing-out and fixed-angle rotors
• Volume graduation for easy reading