Manufactured by a proprietary heat-shock fractionation process, using caprylic acid as an albumin stabilizer. Standard quality for many applications: protein standard, growth promoter in serum-free media for the cultivation of animal cells, supplement in microbiological nutrient media, diluent/stabilizer in diagnostic systems and of isolated enzymes, peptides or antibodies as well as blocking agent to prevent non-specific absorption in immunoassays like Western Blots, ELISA systems.

Ready-to-use colloidal Coomassie® solution that allows protein staining within 15 minutes. Contains no organic solvents and no phosphoric acid. Very high sensitivity (> 5 ng /band) with low background for good quantification and high band sharpness. Suitable for native page and SDS page, MS compatible.

Features & Benefits:

• Staining in only 15 minutes
• Very high sensitivity (> 5 ng /band)
• No use of hazardous chemicals
• Re-usable up to 3 times
• Shelf life: 1 year at room temperature

Assay based on the bichinchoninic acid method. Proteins reduce alkaline Cu(II) to Cu(I). Bichinchoninic acid forms a purple complex with Cu(I) with an absorbance maximum at 562 nm. The absorbance is directly proportional to protein concentration.

• Fast and sensitive assay: linear detection range from 25 - 1000 µg protein/ml
• Easy to use: contains ready-to-use reagents and protein standard
• Compatible with many detergents
• Less binding variation between different proteins than Bradford assay

The assay bases on the bichinchoninic acid method (1). Proteins reduce alkaline Cu(II) to Cu(I). Bichinchoninic acid forms a purple complex with Cu(I) with an absorbance maximum at 562 nm. The absorbance is directly proportional to protein concentration.

• Fast and sensitive assay: linear detection range from 0.5 - 20 µg protein/ml
• Easy to use: contains ready-to-use reagents and protein standard
• Compatible with many detergents
• Less binding variation between different proteins than Bradford assay

Protein-free, polymer-based blocking reagent for fast blocking without loss of sensitivity. With BlueBlock, the specific binding sites remain accessible while nonspecific reactions are suppressed, thus leading to an increase in signal intensity.

• Short incubation times and increased signal intensity
• Complete procedure in only 1 - 2 h with All-in-One protocol
• For colorimetric, chemiluminescence and fluorescence detection systems

For fast and gentle electrotransfer of proteins in Western Blots. The BlueBlot semi-dry blotters form a homogeneous electrical field that guarantees fast and efficient transfer of proteins from gel to membrane. As associated with semi-dry blotting compared to tank blotting less heat is generated for gentle protein transfer. It is fast and requires less buffer. Moreover, all common continuous and discontinuous buffer systems can be applied without any limitations. The electrodes are built into a stable acrylic housing that is resistant to 10 % ethanol and easy to clean. The long-lasting electrodes can be dismounted and cleaned separately.

Features & Benefits:

• Platinum-covered steel net as anode, stainless steel cathode
• Spring-mounted anode allows blotting of thicker gels and gel stacks
• Blotting areas SD11: 11 cm x 11 cm, SD17: 17 cm x 17 cm, SD26: 24 cm x 26 cm
• SD11 expandable to 17 cm x 17 cm with 11835802, SD17 usable for minigels 11 cm x 11 cm with11835801
• Weight: 3 kg / 4 kg (SD26)
• Made in Germany

The BlueVertical™ PRiME™ is a dual minitank system for one or two pre-cast gels and is perfectly suited for protein separations by SDS-PAGE, native PAGE, IEF as well as nucleic acid separations by denaturing or native PAGE.
The instrument consists of an outer buffer tank made of robust, transparent acrylic and the inner running unit. A safety lid closes the top and results in a very compact and robust design.
The outer buffer tank works as a heat sink (passive buffer cooling) and is sufficient for most applications mentioned above.

• Unique clamp system, complete leak-free
• Installation of the gels in seconds
• Made in Germany

Fluorobind PVDF membranes show an excellent protein binding capacity. Suitable for all standard applications in protein analysis, and as well for special applications as fluorescence detection and protein sequencing.

• Ideal for blotting of proteins of lower molecular weight and of peptides
• As well suitable for larger proteins
• High sensitivity with low background in all common detection systems
• High mechanical stability facilitates handling
• Allows multiple stripping of the membrane and harsh washing conditions

For the staining of blotted proteins on nitrocellulose, PVDF membranes and cellulose acetate membranes The reversible staining is ideally suited for subsequent immunoblots (Western blots) or other staining procedures, as Ponceau S can be completely removed.

Features & Benefits:

• Protein staining on membranes
• Completely reversible
• In 3 % Trichloroacetic acid

SERVA Fluo-610 Standard I is a ready-to-use fluorescent labelled protein marker for direct detection in a SDS-PAGE gel or on a membrane in Western Blotting. The marker proteins in the range from 14.4 kDa to 9.4 kDa are labelled with the highly sensitive and stable fluorescent dye Lightning Red. SERVA Lightning Red (cat. no. 43402) is a fluorescent dye for rapid labelling in minutes of proteins prior to SDS PAGE and/or Western Blotting, making any staining and washing steps after electrophoresis unnecessary.

Lightning Red is a fluorescent dye for rapid labeling of proteins prior to SDS PAGE and/or Western blotting, eliminating the need for any staining and washing step after electrophoresis. In Western blotting, the labeled protein samples can be easily used for sample normalization using the total protein signal as a loading control. The dye is fully compatible with mass spectrometry and other subsequent methods.

• Easy and fast labeling prior to SDS PAGE and Western blotting: qualitative analysis in 2-5 min, quantitative analysis 30 min
• Wide protein concentration range: for complex and for purified samples from 1 µg/ml to 20 mg/ml
• No protein concentration determination, purification or concentration steps necessary before or after labeling
• No staining or washing steps after electrophoresis
• Very high sensitivity, very low background
• Wide dynamic and linear range
• Detection by fluorescence imaging device

Highly sensitive fluorescent dye for detection of proteins in e. g. SDS PAGE, native PAGE or 2D gels. The dye does not interfere with immunodetection. Therefore you can stain your gel with SERVA ProteinStain Fluo-R and then proceed with Western Blotting receiving a copy of your gel on the membrane.
The dye can as well be used for pre-staining, just use it in the loading buffer instead of bromophenol blue or other dyes.
It is as sensitive as silver staining, but has superior staining properties, which makes it the first choice for proteomic research. The dye has a good linearity, high contrast and is compatible with MS/MS analysis. The staining can be as well combined with silver staining and DIGE.
Easily soluble in water. The dye is best excited with UV light of wave length 473/488 nm. Excitation with laser light of wave length 532 nm is as well possible, but less sensitive.

Total protein stain for non-denaturing and denaturing 1D and 2D gel electrophoresis and blotting. The dye reversibly binds to lysine, arginine, and histidine residues in proteins and peptides to yield an intensely red-fluorescent product (ʎ?Ex 518 nm, ʎ?Em 610 nm). SERVA Purple HiSens shows highest sensitivity compared to most other protein stains. Even difficult to stain proteins as glycoproteins and lipoproteins can be accurately detected.

• Environmentally friendly, easy to use
• Sensitivity to as low as <20 pg/band
• Linear quantification over 4 orders of magnitude
• Compatible with MS, DIGE-labelling
• After imaging gel can be further processed by Western Blotting

Assay based on the eco-friendly fluorescent dye SERVA Purple. The dye reversibly binds to lysine, arginine, and histidine residues in proteins and peptides to yield an intensely red-fluorescent product. The assay exhibits very low protein binding variation, leading to more accurate protein concentration values.

• Fast and simple - no heating and reduction steps, completed in 1 h
• Compatible with many detergents and reducing agents
• Accurate staining of glyco-, phospho-, hydrophobic proteins and peptides
• Single tube and 96- or 384-well-format for high-thoughput
• Detection limit of 100 ng/ml for peptides and 40 ng/ml for proteins
• Linear quantification over 3 orders of magnitude
• Compatible with downstream applications like 1D- and 2D-PAGE, MS, DIGE-labelling, HPLC

Silver staining kit for easy and rapid staining of proteins after SDS PAGE:
Contains everything needed for fixation and staining.

• Fast staining procedure (45 - 60 min.)
• Very low background
• High sensitivity
• With detailed staining manual
• For 25 applications

To determine the molecular weight of proteins from SDS-PAGE on a
polyacrylamide gel, SERVA offers a range of different types of
pre-stained and unstained protein markers from natural and recombinant origin.
The VisiBlot Standard I has three pre-stained protein bands and seven bands
with IgG binding sites for visualization via antibody detection.


These ready-to-use standards are perfectly suitable as protein markers for your SDS-PAGE. Pre-stained markers are convenient for controlling the separation during the electrophoresis run or the efficiency of protein transfer to a membrane. Since the molecular weight of the proteins differs from that of the unstained markers due to the staining, unstained protein standards are used for accurate molecular weight determination.

SERVA unstained protein standard IV:

• Precise molecular weight determination in SDS-PAGE gel
• Testing of transfer efficiency in Western Blotting

• Tracking of protein separation in the gel
• Estimation of protein sizes

VisiBlot Standard I is a mixture of 10 recombinant proteins of a molecular weight range from 25 kDa to 150 kDa. Protein bands of 25 kDa, 45 kDa and 85 kDa are prestained allowing monitoring of protein separation during SDS PAGE. The remaining five proteins contain several IgG binding sites. Hence marker proteins bind to primary or secondary antibodies used in Western Blotting facilitating easy marker visualization on the transfer membrane. Because the proteins have no chromophore attached, the marker enables accurate molecular weight estimation.
Recommended loading volume for a mini gel is 5 µl/lane.

• Ready-to-use, no reconstitution, further dilution or heating required
• Prestained bands for monitoring electrophoresis and membrane transfer
• Visualization of marker proteins on Western Blots by horseradish peroxidase or alkaline phosphatase-based immune-detection methods
• Molecular weight determination of proteins detected on transfer membrane

Ready-to-use, non-toxic, highly sensitive substrate solution for detection of alkaline phosphatase (AP) in membrane assays. Forms dark purple precipitates at the sites of AP activity on membranes.

• Rapid precipitate formation due to high activity
• High contrast due to very low background
• Long term stability at room temperature
• No significant fading after reaction stop

Ready-to-use, non-toxic, highly sensitive substrate solution for detection of horseradish peroxidase (HRP) in membrane assays. Forms dark blue precipitates at the sites of HRP activity on membranes.

• Rapid precipitate formation due to high activity
• High contrast due to very low background
• Long term stability at room temperature
• No significant fading after reaction stop

The SERVAGel™ TG PRiME™ pre-cast gels for vertical SDS have a modified Tris/Glycine buffer system (Laemmli buffer system), which not only offers a clearly extended shelf life compared to classical tris-glycine gels according to Laemmli, but also allows shorter electrophoresis times. The gels allow a separation of 3 kDa up to 200 kDa.

Features & Benefits:

• Fast separation, high resolution
• Separation range down to 3 kDa and up to over 200 kDa
• Separation pattern comparable to standard Laemmli gels
• Long shelf life (> 1 year), ideal suited for storing of gels in the lab
• Available as homogeneous and gradient gels
• All gels are also available with 10, 15 or 2D sample wells

Single tube format assay kit for protein quantification. The assay bases on the precipitation of proteins as insoluble dye complexes with acidic, ethanolic amido black 10B solution (1,2). After precipitation the protein-dye complexes are spinned down. The pellet is washed and resolubilized. The thereby released dye amount is measured at 624 nm.

• Precise, reproducible, reliable assay data
• Completed in only 45 min.
• No interference with detergents or reducing agents
• Detection range starts as low as 2 µg protein

Skim milk powder is used as a blocking reagent in immunological assays like Western Blotting or ELISA. It is as well suitable for blocking of nitrocellulose filters in cDNA cloning.
It is not suitable for biotin/streptavidin detection systems, because milk contains biotin.

The Xpress Blotting Buffer is a ready-to-use buffer reagent for the fast and efficient semi-dry transfer of high and low molecular weight proteins in only 15 min. The buffer system is compatible with nitrocellulose and PVDF membranes. Sufficient for at least 40 vertical mini SDS PAGE gels.

Kit for fast Semi-Dry Western Blotting of 10 vertical mini SDS PAGE gels.
SERVA Xpress Blotting Buffer is a ready-to-use buffer reagent for the fast and efficient semi-dry transfer of high and low molecular weight proteins in only 15 min.
The use of SERVA's newly developed Blotting Fleece instead of blotting paper allows an efficient, undisturbed transfer in a short time. The buffer system is compatible with nitrocellulose and PVDF membranes.

Content:
250 ml 10x SERVA Xpress Blotting Buffer
20x Blotting Fleece sheets (size 80 x 85 mm)
10x Connection Paper (size 80 x 85 mm)