Protein blotting

Supported Nitrocellulose transfer membranes combine the binding characteristics of nitrocellulose membranes with the strength of nylon membranes. These membranes are used for DNA/RNA transfers requiring low background or for multiple hybridizations. For use with chemiluminescence and radiographic detection systems. BSA binding capacity up to 100 µg/cm².

The safety data sheets of these products are available under www.llg-labware.com.

Nitrocellulose membranes with a 0.2 µm pore size, offering excellent sensitivity, resolution and low background for Western blot protocols.

Features & Benefits:

• Low background and excellent signal-to-noise ratios, without the need for stringent washing conditions
• Compatible with a variety of Western blot labeling and detection systems
• Risk of ”blow-through“ reduced by high binding capacity of small proteins (< Mr 20 000)
• Optimized for chemiluminescent and fluorescent detection
• Long shelf life of bound proteins to the transfer membrane

Nitrocellulose membranes with a 0.45 µm pore size for use in Western blot protocols. Excellent sensitivity, resolution, and low background. for a wide range of laboratory applications.

Features & Benefits:

• Low background and excellent signal-to-noise ratios
• Compatible with a variety of labelling and detection systems
• High binding capacity
• Suitable for proteins with a wide range of molecular weights
• Ideal tool for your antibody purification and analysis workflow
• Long shelf life of bound proteins

PVDF blotting membrane with 0.45 µm pore size, hydrophobic and of high physical strength, for use with standard colorimetric and chemiluminescent detection methods for proteins of < Mr 20 000.

Features & Benefits:

• Protein binding capacity over 200 μg/cm² for easy signal detection.
• High protein retention even after harsh washing steps.
• High protein capture during transfer minimizes sample loss.
• Suitable for stripping and reprobing

Double-coated, high sensitivity film optimized for use in all blue and green light chemiluminescence systems for Western blot analysis and validated for use with Amersham ECL™ and CDP-Star.

Features & Benefits:

• High sensitivity Western blot membrane to detect low concentrations of nucleic acid and protein targets
• Clear background for excellent contrast and improved band resolution
• Produces publication-quality images
• Easy to read and simple-to-use
• To be processed in automatic processors or manually in tanks and trays using most common X-ray film developers and fixers

Nitrocellulose blotting membrane with 0.45 µm pore size, for excellent sensitivity, resolution and low background.

Features & Benefits:

• High binding capacity (162 - 180 µg IgG/cm²)
• Suitable for proteins of a wide range of molecular weights
• 100% pure nitrocellulose membrane for use with all ECL Western blotting systems and protein blotting applications.
• Available in popular precut sizes for laboratory application

GB 003, GB 005. High purity. Very absorbent. Easy to use. Suitable for all protein and nucleic acid blotting techniques.

Low fluorescent, hydrophobic blotting membrane for protein transfer. Optimized for fluorescence detection in Western blotting applications.

• Protein binding capacity over 200 µg/cm²
• Higher sensitivity due to low background fluorescence
• Compatible with many blocking agents, transfer buffers and total protein blot stains
• Available in the standard precut sizes for laboratory applications

PVDF blotting membrane with 0.2 µm pore size, hydrophobic with high physical strength, for use with standard colorimetric and chemiluminescent detection methods. The small pore size of the membrane minimizes "blow-through" and increases protein binding over a wide range of molecular weights.

Features & Benefits:

• High protein binding capacity for easy signal detection
• High protein retention even after harsh washing steps
• Maximum capture of proteins during transfers minimizes sample loss
• 0.2 μm pore size for higher surface area, resulting in better binding of low molecular weight proteins
• Highly suitable for stripping and reprobing

Hydrophobic Western blotting membrane specifically designed for protein sequencing applications and solid-phase amino acid analysis.

• High protein binding capacity
• Chemical resistance needed for N-terminal sequencing
• High protein retention even after harsh washing steps
• Minimizes "blow-through" and increases protein binding
• Available in popular sizes for laboratory application

Pure nitrocellulose with extreme mechanical stability - gives excellent useability. For protein transfer and DNA/RNA transfer techniques. Optimum signal/background ratio.

Nitrocellulose blotting membrane, with 0.2 µm pore size, for excellent sensitivity, resolution, and low background.

Features & Benefits:

• Low auto-fluorescence across a wide range of excitation/emission wavelengths makes the membrane suitable for fluorescence-based immunodetection and multiplexing.
• High binding capacity (173 - 203 µg IgG/cm²) of small proteins (< Mr 20 000) reduces risk of "blow-through"

Reinforced 0.45 µm protein blotting membrane for multiple reprobings.

Features & Benefits:

• High binding capacity
• Versatile, robust and excellent signal-to-noise ratios
• Optimal for applications when stripping and reprobing is required
• Suitable for proteins of a wide range of molecular weights

Recommended for blotting techniques with gels, like Southern, Northern and Western Blots, even Semidry-Blots of proteins

• Made from ultrapure cotton linters and cellulose, entirely without additives to ensure that no contamination will occur during the transfer steps
• Tested specifically for chromatographic and blotting techniques to ensure high and uniform capillary actions
• High wet strength for safe handling

These Semi Dry Blotters offer rapid transfer times for DNA, RNA and protein blotting; typically 15 to 30 minutes. All units can be used for all types of blotting: western, southern and northern via uncomplicated buffer and set up procedures and are compatible with gel thicknesses from 0.25 up to 10mm without the need for additional equipment. Each unit is compatible with their respective omniPAGE vertical gel system. Semi Dry Blotting has the added benefit of economic transfers due to very low buffer volumes - typically only a few millilitres of buffer are required per transfer. These Semi-Dry Blotters utilise a screw down lid, which secures the blot sandwich and allows complete control of pressure ensuring even transfer. The electrodes, comprising platinum coated anode and stainless steel cathode, will exhibit practically no corrosion and so provide many years of trouble free use. Uniform heat dispersion across the blot sandwich ensures stable transfer times and no heat induced sample loss or transfer distortions. Being translucent, it allows viewing of the blot sandwich to ensure correct positioning and transfer is occurring correctly. Electrode plates are fully separated to prevent arching or damage.

The TurboBlotter is very easy to set up, and works without power or vacuum source. Offers high resolution transfer of DNA and RNA with unique downward capillary transfer in one hour. Reusable blotting device requires less buffer and blotting paper. Each TurboBlotter Kit includes: transfer device, 5 sheets of membrane, 40 sheets of GB002, 100 sheets of GB004, and 5 wicks of GB002. Convenient refill packs also available.

In contrast to nitrocellulose, Immobilon membranes have a number of advantages. An optimized coloring capacity, a broad solvent compatibility and a lower background make the membranes your solution. Immobilon membranes do not rupture or curl and can be cut without breaking. Reprobing is also possible many times. The Immobilon FL membranes are ideal for fluorescence applications thanks to their optimized transfer membrane.

Features & Benefits

• Extremely weak background improves the sensitivity of all fluorescence detection protocols
• Compatible with all commonly used fluorescence probes, at all excitation and emission wavelengths
• Ideal for multiplexing and chemifluorescence applications

An all-in-one blocking solution for Western blot analysis. In one step, blocking, primary and secondary antibodies hybridization as well as enhancing the signal.

• Enhance antibody signal
• Universal antibody diluent
• Effective with any ECL substrates
• Compatible with PVDF and NC membrane
• Improve protein detection

Extremely pure, very absorbent, smooth paper for use in all protein and nucleic acid blotting techniques.

In contrast to nitrocellulose, Immobilon membranes have a number of advantages. An optimized coloring capacity, a broad solvent compatibility and a lower background make the membrane their solution. Immobilon membranes do not rupture or curl and can be cut without breaking. Reprobing is also possible many times. The Immobilon P membrane is particularly suitable for proteins with a molecular weight greater than 20 kDa.

Features & Advantages

• 0.45 µm Pore size
• Suitable for Western Blotting
• Millipore Rapid Immunodetection Protocol reduces detection time by up to 2 hours

In contrast to nitrocellulose, Immobilon membranes have a number of advantages. An optimized coloring capacity, a broad solvent compatibility and a lower background make the membranes your solution. Immobilon membranes do not rupture or curl and can be cut without breaking. Reprobing is also possible many times. The Immobilon PSQ membranes are particularly suitable for blotting proteins with a molecular weight below 20 kDa. They also offer higher protein adsorption and therefore better results in protein sequencing than with other membranes.

Features & Benefits

• 0.2 µm pore size, textured surface
• Prevent the migration of proteins with lower molecular weight through the membrane