Analysis (Microbiology)

Media for bacteria
Luria Bertani (Miller):

• Standard high salt content media for E. coli cultivation
• Ideal for plasmid preparation

Luria Bertani (Lennox):

• Standard medium with low salt content for the cultivation of E. coli
• Ideal for cultivating recombinant E.coli strains

Terrific Broth:

• Rich content media for the high density cultivation of E. coli
• Ideal for protein expression and plasmid preparation

2xYT Broth:

• Nutritive medium for the cultivation of recombinant E. coli strains and for the growth of filamentous bacteriophages

Agar
Bact. Agar:

• High quality agar used for the preparation of culture media and other bacteriological applications

Luria Bertani Agar (Miller):

• Standard Agar based on Luria Bertani (Miller) media with high salt content
• Ideal for growth and maintaining especially of E. coli strains used in molecular microbiology procedures

Luria Bertani Agar (Lennox):

• Standard Agar based on Luria Bertani (Lennox) media with low salt content
• Ideal for growth and maintaining especially of recombinant E. coli strains

Alicyclobacillus Detection Agar:

• Agar for the cultivation of Aliclyciclobacillus spp. in fruit juices

Orange Serum Agar:

• Agar for isolation, cultivation and enumeration of acid-tolerant microorganisms

PCA Agar:

• Non-selective agar for the cultivation and standardized enumeration of aerobic microorganisms

Media for yeasts
YPD-Broth:

• Standard medium for the cultivation of Saccharomyces cerevisiae and other yeasts

Media components
Yeast Extract:

• Concentrate of the water-soluble portion of autolyzed Saccharomyces cerevisiae cells
• Non-animal product, used widely for non-animal formulations of bacterial, fungal, mammalian and insect cell culture

Tryptone:

• Pancreatic digest, tryptone from casein
• Excellent nutrient for use in culture media for producing antibiotics, toxins, enzymes and other biological products

Casein Peptone:

• Pancreatic digest, peptone from casein
• Ideal for use in fermentation applications and microbiological culture media

For numerous micro- and molecular biological applications.
The safety data sheets of these products are available under www.llg-labware.com.

The Lovibond® Dipslides are designed to test liquids as well as surfaces. They are ideal for use in many applications including evaporative cooling towers, closed water circuits, cutting fluids, food testing, and many more.
These dual-sided Dipslides are available in a wide range of different media types.

Scope of supply Sharpie® Set: Dual-tip pen set (fine and extra fine), 8 colours (black, berry, lime, navy, orange, purple, sky blue, and turquoise).

Suitable for colony counter ecount Cat. No. 9.521 783.

Multi-function electronic counter with a Sharpie^® pen for marking Petri dishes to prevent missing or double-counting colonies. Light, ergonomic body is balanced for easy manipulation. For easy counting of bacteria and mould fungus colonies.
Counting contol via membrane keys.

• Incl. thumb button which allows you to use the eCount as a digital tally counter
• Count up, then count down verification
• Display the total and keep in the memory up to 32 separate counts
• Disable the counter so you can write totals and notes
• Verify each count with either a bleep or LED Flash
• 5 years warranty (excludes battery)

Accessories Permanent marker Sharpie®

Effective and practical counter with a sturdy, chrome finished housing. Ideal for counting blood cells, bacterial colonies, drops of liquid or any other repetitive step with a tally level up to 9999. TALLY COUNTER - "handy" Features a quick-reset knob, lens window for easy reading, and a finger ring for a secure grip.

BTX Cuvettes Plus are designed for use in electroporation and electrofusion of bacteria, yeast, insect, plant and mammalian cells. Each sterilized Cuvettes Plus package includes a disposable cuvette and a transfer pipette, which is used for removal of the sample after electroporation. The cuvettes are molded with embedded polished aluminum electrodes, and gamma irradiated for guaranteed sterility.
Three electrode gap sizes are available:

• 1mm for bacteria and yeast
• 2mm for all cell types
• 4mm for mammalian cells

BTX cuvette Plus can obtain high field strengths up to 25.0 kV/cm. Round caps are leak resistant and allow for quick and easy removal. For research use only. Can be used with electroporation systems from different manufacturers (not suitable for units from Eppendorf).

Accessories Colony Counter CC-200, manual

Accessories schuett count Colony Counter

Scan^® 300 is an automatic colony counter that includes the essential features for Petri dish counting. It is specifically adapted to the usual media in food microbiology (PCA, MRS...). With a CCD camera with 1 Megapixel - zoom x 28. It counts colonies on a Petri dish in less than half a second, with a minimum colony size of 0.1 mm. For the detection of colonies on round pour, surface, spiral and circle mode plated dishes Ø 55 - 90 mm.

Scan^® 500 is a high-tech automatic colony counter. It counts colonies on a Petri dish in less than half a second and gives you quick, accurate, complete and traceable reading of the results. With its CCD camera and a powerful software, it connects to a PC via a Firewire connection automatically export results to Excel.

Scan^® 1200 is a high resolution automatic colony counter that offer quick, and traceable counting results. Widely used in food microbiology, it counts colonies and automatically saves all data to Excel. Equiped with a digital camera and a powerful software, it connects to a PC via a Firewire connection. Scan^® 1200 counts all colonies, even on smallest poured, spread and Spiral^® Petri dishes; on PetriFilm™ and RIDA™ Count/Sanita-kun™, on filtration membrane and Compact Dry™.

• Pressure sensitive counting
• Pressure can be adjusted
• Audible confirmation
• Choice of light or dark background
• Bright white energy saving LED lighting
• Average count facility
• With BioCote^® antimicrobial protection
• Connectivity to printer or computer

Scope of supply: Device with two Wolffhuegel graticules and dish centering adapters to facilitate use with 50 to 90 mm dishes.

Please order magnifiers and printers separately.

Specification
Digital display:	3 digit LED
Lighting:	White LED
Magnifier glass:	1.7x and 3x (option)
Counter:	0 to 999
Petri dishes:	diam. 50 to 90 mm
Dimensions (W x D x H):	310 x 300 x 140 mm
Weight:	1.5 kg
Power supply:	120-230 V, 50/60 Hz, 70 W
Warranty:	3 years

Accessories Accessories for Colony Counter CC-200 Accessories

Scan^® 4000 is an ultra HD automatic colony counter and inhibition zone reader for high resolution color reading of colonies and inhibition zones. Adapted to all sizes of Petri dishes and all media, its lighting system guarantees a great user comfort, high accuracy and excellent reproducibility. Automatic lighting system with 6 combinations, top and/or bottom light, white or black background. Automatic counting and inhibition zone measurement with manual control

• All stainless steel body
• Counting: 1000 colonies per second
• Minimum size of colony: 0.05 mm
• Color detection: 7 colors on the same dish
• Long-life white LEDs / Dark Field
• Counting on e.g. Petri dishes 55 to 150 mm, PetriFilm™ and other plates
• Inhibition zone reading on 90 to 100 mm round dishes and 120 mm square dishes
• Maximum number of antibiotics: up to 16 antibiotics on a square 120 mm dish
• Reading time: measurement of 16 inhibition zones between 2 and 4 seconds maximum
• Automatic separation of confluent colonies
• Results: Scan^® file, PDF report, jpeg, png, bmp, Excel™
• LIMS and USB connection
• Software available in English, French, Spanish, Japanese, Chinese, Russian

Specifications
Camera:	Color HD CCD camera and digital zoom x64
Camera Resolution:	5 mega-pixels
Lighting:	LED/Dark field
Min. colony size:	0.05 mm
Counter:	1000 colonies per second
Culture dishes:	55 to 150 mm, 90 to 100 mm round dishes, 120 mm square dishes
Dimensions (W x D x H):	470 x 470 x 640 mm
Weight:	24.4 kg
Power supply:	100-240 V, 50/60 Hz

Easy-to-use, respirometric measuring system for determining the biological oxygen demand (BOD). The method is based on changes of pressure in a closed system using measuring heads attached to the sample bottles. The BOD can be determined simultaneously in up to 6 or 12 samples.

• No dilution of the sample required
• Direct reading of the BOD, the time course and the intermediate values on the graphical display of the measuring heads
• Input of the used sample volume directly at the measuring head
• Monitoring time between 1 and 7 days
• Permanent display of the current status at each measuring head
• Coloured measuring heads with adjustable, numerical sample identification

Scope of supply: OxiTop^®-i IS 6: 6 x measuring heads (3 x blue, 3 x grey), 6 x sample bottles PF600, 6 x rubber quivers, CO₂ absorber, nitrification inhibitor, overflow measurement flasks, 6 x stirring rods, stirring rod remover, stirring platform SI 6
OxiTop^®-i IS 12: 12 x measuring heads (6 x blue, 6 x grey), 12 x sample bottles PF600, 12 x rubber quivers, CO₂ absorber, nitrification inhibitor, overflow measurement flasks, 12 x stirring rods, stirring rod remover, stirring platform SI 12

An incubator is required to operate the OxiTop^®-i IS. Please order separately.

Specifications
Pressure range:	500 ... 1250 hPa
BOD range:	0 ... 4000 mg/l
Display:	Graphical LCD, background-illuminated
Power supply of measuring heads: Power supply of stirring platform:	1 x CR 2450 100 ... 240 V, 50/60 Hz

Accessories Measuring heads OxiTop®-i Accessories Stirring platforms OxiTop® for BOD measurement Alternative BOD measuring system OxiTop®-IDS for determination of aerobic degradation Alternative BOD Measuring system OxiTop®-IDS for determination of soil respiration Alternative

BOD measurement system for all types of respirometric measurements, e.g. soil respiration, microbiological activity measurement, biogas determination as well as extended BOD measurement for the industrial waste water plants and further degradation tests in 3 selectable measuring types, BOD standard, BOD special and pressure.
With the proven Bluetooth^® connectivity and the convenient operation via MultiLine^® multi parameter instrument Multi 3620 IDS or Multi 3630 IDS. Using a Multi 3620 IDS or Multi 3630 IDS in BOD special mode the BOD measuring range extends to 400000 mg/l. For an already existing Multi 3620 or 3630 IDS, a firmware update is sufficient. Herewith beneath the OxiTop^® applications also measurement of pH, conductivity, solved oxygen and turbidity are available. All heads can be used independently for routine BOD measurements from 1 to 7 days.

• Bluetooth^®connectivity
• Direct calculation of all results in the meter
• Representation of curves for monitoring
• Parallel measurement of different examinations and methods
• Export to data acquisition programs

These tests quantify the biological degradability of chemicals. The OxiTop^®-IDS A12 is also suited for high dilutions.

• Respirometric degradability tests according to OECD 301F/DIN EN ISO 9408
• Versatile measurement by using the BOD special mode

Scope of supply:
OxiTop^®-IDS Set 6: 6 x OxiTop^®-IDS measuring heads, 6 x bottles PF600 (510 ml), 6 x rubber sleeves, nitrification inhibitor, overflow measuring flask , 6 x magnetic stirring bars, stirring bar retriever, stirring platform IS 6
OxiTop^®-IDS Set 12: 12 x OxiTop^®-IDS measuring heads, 12 x bottles PF600 (510 ml), 12 x rubber sleeves, nitrification inhibitor, overflow measuring flask, 12 x magnetic stirring bars, stirring bar retriever, stirring platform IS 12
Erweiterungssatz für OxiTop^®-IDS 6/12: 6 resp.12 OxiTop^®-IDS measuring heads, 6 resp.12 bottles, universal power adapter, 6 resp.12 rubber sleeves, 6 resp.12 magnetic stirring bars, stirring platform IS 6 resp. IS 12
OxiTop^®-IDS A6: 6 x OxiTop^®-IDS measuring heads, 6 x bottles PF45/1000 (1000 ml), 6 x adapter OxiTop^® AD/SK, nitrification inhibitor, 6 x magnetic stirring bars, stirring bar retriever, stirring platform IS 6-Var
OxiTop^®-IDS A12: 12 x OxiTop^®-IDS measuring heads, 12 x bottles PF45/250 (250 ml), 12 x adapter OxiTop^® AD/SK, nitrification inhibitor, 12 x magnetic stirring bars, stirring bar retriever, stirring platform IS 12

Please order CO₂ absorber NHP 600 (9.303 403) separately.
The IDS multi parameter measuring instruments Multi 3620 IDS Set WL or Multi 3630 IDS Set WL are required. Please order separately.

Specifications
Pressure range:	500 ... 1250 hPa
BOD range:	0 ... 4000 mg/l
Measurement period:	0,5 h ... 180 d
Memory:	360 values
Display:	LCD

Accessories Multi parameter measuring instruments Multi 3620/3630 IDS SET WL for BSB measuring system OxiTop® IDS Accessories Wireless measuring heads OxiTop® IDS Accessories Wireless module for WTW® measuring instruments Accessories Stirring platforms OxiTop® for BOD measurement Accessories Sample bottles for BOD measurement system OxiTop® Alternative BOD Measuring system OxiTop®-IDS for determination of soil respiration Alternative  Alternative BOD measurement system OxiTop®-i IS 6 / 12

BOD measurement system for all types of respirometric measurements, e.g. soil respiration, microbiological activity measurement, biogas determination as well as extended BOD measurement for the industrial waste water plants and further degradation tests in 3 selectable measuring types, BOD standard, BOD special and pressure.
With the proven Bluetooth^® connectivity and the convenient operation via MultiLine^® multi parameter instrument Multi 3620 IDS or Multi 3630 IDS. Using a Multi 3620 IDS or Multi 3630 IDS in BOD special mode the BOD measuring range extends to 400000 mg/l. For an already existing Multi 3620 or 3630 IDS, a firmware update is sufficient. Herewith beneath the OxiTop^® applications also measurement of pH, conductivity, solved oxygen and turbidity are available. All heads can be used independently for routine BOD measurements from 1 to 7 days.

• Bluetooth^®connectivity
• Direct calculation of all results in the meter
• Representation of curves for monitoring
• Parallel measurement of different examinations and methods
• Export to data acquisition programs

Respirometric measuring system for 6 measuring heads for determination of aerobic soil respiration, OxiTop^®-IDS B6M-2.5 acc. to AT4 guideline. These sets are suitable for forecast, survey and monitoring of clean-up operations as well as measurement of bio-degradation of pesticides, fungicides, insecticides and fertilizers for example.

Scope of supply:
OxiTop^®-IDS B6: 6 measuring heads, 6 sample bottles PF45/500 (500 ml), 6 adapters OxiTop^® AD/SK
OxiTop^®-IDS B6M: as OxiTop^®-IDS B6, but with 6 vessels MG 1,0 (1000 ml)
OxiTop^®-IDS B6M-2.5: as OxiTop^®-IDS B6M, but with 6 vessels MG 2,5 (2500 ml) and Soda-Lime CO₂ absorbent

Please order CO₂ absorber NHP 600 (9.303 403) separately.
The IDS multi parameter measuring instruments Multi 3620 IDS Set WL or Multi 3630 IDS Set WL are required. Please order separately.

Specifications
Pressure range:	500 ... 1250 hPa
BOD range:	0 ... 4000 mg/l
Measurement period:	0,5 h ... 180 d
Memory:	360 values
Display:	LCD

Accessories Multi parameter measuring instruments Multi 3620/3630 IDS SET WL for BSB measuring system OxiTop® IDS Accessories Wireless measuring heads OxiTop® IDS Accessories Wireless module for WTW® measuring instruments Accessories Stirring platforms OxiTop® for BOD measurement Accessories Sample bottles for BOD measurement system OxiTop® Accessories Adapter AD/SK for BOD measuring system OxiTop® Accessories Measuring vessels for BOD measuring system OxiTop® Accessories  Alternative BOD measuring system OxiTop®-IDS for determination of aerobic degradation Alternative  Alternative BOD measurement system OxiTop®-i IS 6 / 12

Designed for reliable and efficient counting of bacterial colonies and bacteriophage plaques growing on nutrient agar or nutrient discs/filter disks in Petri dishes. Provides ergonomic and relaxed working.

• For Petri dishes with Ø 90 mm (Ø 60 or 150 mm optional)
• Individually adjustable lighting, pressure sensitivity of the counter plate and volume
• LED lighting technology, absolutely glarefree and gentle on the eyes
• Three types of illumination: from below, from the side and incident light (optional)
• High colony transparency and differentiation (agar or nutrient discs/filter), even with transparent colonies
• No heating of the samples
• Natural colour reproduction
• Easy counting by marking the colonies (with any felt pen) on the Petri dish
• Acoustic counter control (with volume control)
• 4-digit LED-display (0 - 9999), clearly arranged foil keypad
• Average count calculation, data transfer via USB to PC. Imported in Excel, for further processing, storage and printing

Scope of supply: For 90 mm dia. Petri dishes: adapter (transparent) for illumination from below, adapter (black/white) for illumination from side, contrast disc (black), USB-cable, marker-pen with holder.

For enumeration of more than 50 Petri dishes per day, we recommend: schuett colonyQuant automatic colony counter (sample chamber, PC / laptop, software-license): The schuett colonyQuant is equipped with a high-resolution auto focus / auto-zoom colour camera. Evaluation of Petri dishes with agar Petri film, filters or nutrient pads, spiral plating or Hemmhof analysis. Storage of counting methods, documentation of results.

Suitable for automatic counting and documentation of Petri dishes or nutrient pads, covered with smallest colonies (min. 0.05 mm diameter), mixed cultures. Connection to existing LIM system possible. Test evaluation of customer-specific plates in the customer"s own laboratory possible before purchase.

Specifications
Dimension (W x D x H):	260 x 250 x 130 mm
Height with integral support stand:	300 mm
Weight:	ca. 1.5 kg

Accessories Accessories for schuett count Colony counter

With double ruling. Calibratable. Cell depth 0.1 mm. Same ruling as Improved Neubauer, but the large square in the centre is divided into 16 squares each with a side length of 0.25 mm. Unlike the more modern Improved Neubauer counting chambers, the counting area of each group square is limited by the outermost of the triple border lines.

Alternative Counting chamber, Improved Neubauer, bright line Alternative Counting chamber, Improved Neubauer Alternative Counting chamber, Fuchs-Rosenthal

BLAUBRAND^®. Double ruling. With or without snap-on clips. Cell depth 0.1mm. Combination of the Bürker and Thoma systems. The chamber depth is 0.1 mm. The ruling shows 9 large squares of 1 mm² each. The large squares are subdivided into 16 group squares with 0.2 mm sides. In the central large square, each group square is subdivided into 16 mini squares with 0.05 mm sides (= 0.0025 mm²). For research and development only.

These test kits are specially developed for testing boiler, cooling and industrial process water. They make use of both colorimetric and titrimetric techniques. Each test kit contains all the necessary chemicals and reagents in liquid
or powder form to conduct the tests. The detailed instructions contain a step-by-step explanation of the test procedure. The kits are supplied in a sturdy, compact plastic case.

• Fast quantitative determination
• For testing boiler, cooling and industrial process water
• Suitable for field and laboratory testing
• Cost-effective use due to keenly priced refill packs

Transparent polystyrene microtiter plates for almost all microvolume applications, such as high-throughput screenings, research applications, storage, sample or reagent transfer, serial dilution, hemagglutination, blood typing and complement fixation.

• Transparent plates for colourimetric applications
• Standard size 128 × 86 mm
• Different bottom shapes available

Accessories Lids for 96-well and 384-well Plates Accessories Sealing Tapes for MultiWell Plates Alternative V96 MicroWell™ Plates, PS

The ultrafiltration unit is designed for volumes from 15 ml … 100 µl. The vertical twin membranes enable fast and high sample concentration and almost complete sample recovery. The pipette-friendly dead-stop reservoir eliminates the risk of centrifuging the concentrate to dryness and allows easy recovery of the highly concentrated sample. Samples up to 15 ml can be processed in swing-out rotors or 11 ml in fixed-angle rotors, which are suitable for 50 ml centrifuge tubes with a conical bottom.

• Up to 100 times sample concentration
• High flow rates due to PES membranes
• Volume graduation for easy reading

Membrane surface:	7,1 cm²
Max. rcf.:	4000 x g
Hold-up volume:	10 µl

Alternative Centrifugal concentrators, Vivaspin 20